Mouse Cyclophilin B Monoclonal Antibody | anti-PPIB antibody

Cyclophilin B Monoclonal Antibody (2B10)

Cat. Num. AAA30826

• Gene Names: PPIB; OI9; CYPB; SCYLP; CYP-S1
• Reactivity: Human, Rat, Mouse
• Applications: Immunofluorescence, Immunocytochemistry, Western Blot, Immunohistochemistry
• Purity: The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
• Synonyms: Cyclophilin B, Antibody; Cyclophilin B Monoclonal Antibody (2B10); Peptidyl-prolyl cis-trans isomerase B (PPIase B)(EC 5.2.1.8)(CYP-S1)(Cyclophilin B)(Rotamase B)(S-cyclophilin)(SCYLP); PPIB; CYPB; Peptidyl-prolyl cis-trans isomerase B; PPIase B; CYP-S1; Cyclophilin B; Rotamase B; S-cyclophilin; SCYLP; anti-PPIB antibody

• Host: Mouse
• Reactivity: Human, Rat, Mouse
• Clonality: Monoclonal
• Clone Number: 2B10
• Specificity: Cyclophilin B protein detects endogenous levels of Cyclophilin B
• Purity/Purification: The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
• Form/Format: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-PPIB antibody: Immunofluorescence (IF), Immunocytochemistry (ICC), Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P),
• Application Notes: IF: 1:50-200; WB: 1:1000-2000; IHC: 1:50-300
• Immunogen: Synthetic Peptide of Cyclophilin B
• Modification: Unmodified/Total
• Preparation and Storage: Store at -20 degree C/1 year

IF (Immunofluorescence)

(Immunofluorescence analysis of rat-spleen tissue. 1.Cyclophilin B Monoclonal Antibody(2B10)(red) was diluted at 1:200(4 degree C.overnight). 2. Cy3 labled Secondary antibody was diluted at 1:300(room temperature. 50min).3. Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B)

IF (Immunofluorescence)

(Immunofluorescence analysis of human-lung tissue. 1.Cyclophilin B Monoclonal Antibody(2B10)(red) was diluted at 1:200(4 degree C.overnight). 2. Cy3 labled Secondary antibody was diluted at 1:300(room temperature. 50min).3. Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B)

IF (Immunofluorescence)

(Immunofluorescence analysis of Hela cell. 1.Calmodulin Polyclonal Antibody(green) was diluted at 1:200(4 degree overnight). (red) was diluted at 1:200(4 degree overnight). 2. Goat Anti Rabbit Alexa Fluor 488 was diluted at 1:1000(room temperature. 50min). Goat Anti Mouse Alexa Fluor 594 was diluted at 1:1000(room temperature. 50min).)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded Rat-lung tissue. 1.Cyclophilin B Monoclonal Antibody(2B10) was diluted at 1:200(4 degree C.overnight). 2. Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C.20min). 3.Secondary antibody was diluted at 1:200(room temperature. 30min). Negative control was used by secondary antibody only.)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded Mouse-lung tissue. 1.Cyclophilin B Monoclonal Antibody(2B10) was diluted at 1:200(4 degree C.overnight). 2. Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C.20min). 3.Secondary antibody was diluted at 1:200(room temperature. 30min). Negative control was used by secondary antibody only.)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded Human-uterus tissue. 1.Cyclophilin B Monoclonal Antibody(2B10) was diluted at 1:200(4 degree C.overnight). 2. Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C.20min). 3.Secondary antibody was diluted at 1:200(room temperature. 30min). Negative control was used by secondary antibody only.)

WB (Western Blot)

(Western blot analysis of 1) Jurkat. 2)293T. 3)Rat Liver Tissue. 4)3T3 with Cyclophilin B Mouse mAb diluted at 1:2.000.)

Related Product Information for anti-PPIB antibody

The protein encoded by this gene is a cyclosporine-binding protein and is mainly located within the endoplasmic reticulum. It is associated with the secretory pathway and released in biological fluids. This protein can bind to cells derived from T- and B-lymphocytes, and may regulate cyclosporine A-mediated immunosuppression. Variants have been identified in this protein that give rise to recessive forms of osteogenesis imperfecta. [provided by RefSeq, Oct 2009]

Product Categories/Family for anti-PPIB antibody

Primary Antibodies

NCBI and Uniprot Product Information

• NCBI GI #: 181250
• NCBI GeneID: 5479
• UniProt Accession #: P23284; Q6IBH5; Q9BVK5; A8K534
• Molecular Weight: 23,743 Da
• NCBI Official Full Name: cyclophilin, partial
• NCBI Official Synonym Full Names: peptidylprolyl isomerase B (cyclophilin B)
• NCBI Official Symbol: PPIB
• NCBI Official Synonym Symbols: OI9; CYPB; SCYLP; CYP-S1
• NCBI Protein Information: peptidyl-prolyl cis-trans isomerase B; PPIase B; rotamase B; S-cyclophilin; cyclophilin-like protein
• UniProt Protein Name: Peptidyl-prolyl cis-trans isomerase B
• UniProt Gene Name: PPIB
• UniProt Synonym Gene Names: CYPB; PPIase B; SCYLP
• UniProt Entry Name: PPIB_HUMAN