Mouse anti-Human Cystatin A Monoclonal Antibody | anti-CSTA antibody

Cystatin A

Cat. Num. AAA215441

• Gene Names: CSTA; AREI; PSS4; STF1; STFA
• Reactivity: Human
• Applications: Immunohistochemistry
• Purity: Purified Ab with BSA and Azide at 200ug/ml or Purified Ab with BSA and Azide at 200ug/ml or Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Synonyms: Cystatin A, Antibody; Cystatin A; STF1; STFA; AREI; cstA; Stefin A; anti-CSTA antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2b, kappa
• Clone Number: CSTA/3553
• Specificity: The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins, and kininogens. This gene encodes a stefin that functions as a cysteine protease inhibitor, forming tight complexes with papain and the cathepsins B, H, and L. The protein is one of the precursor proteins of cornified cell envelope in keratinocytes and plays a role in epidermal development and maintenance. Stefins have been proposed as prognostic and identification tools for cancer.
• Purity/Purification: Purified Ab with BSA and Azide at 200ug/ml or Purified Ab with BSA and Azide at 200ug/ml or Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Form/Format: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 98

• Applicable Applications for anti-CSTA antibody: IHC (Immunohistochemistry)
• Immunogen: Recombinant fragment of Human CSTA protein (around aa 1-98) (exact sequence is proprietary)
• Cellular Localization: Cell Surface, Cytoplasmic and Nuclear
• Hu-chromosome Location: 3Q21.1
• Positive Control: Human Tonsil or Prostate tissue (IHC).
• Preparation and Storage: Antibody with azide: store at 2 to 8 degree C.; Antibody without azide: store at -20 to -80 degree C.; Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Application Data

(Analysis of Protein Array containing more than 19,000 full-length human proteins using Cystatin A Mouse Monoclonal Antibody (CSTA/3553). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)

SDS-PAGE

(SDS-PAGE Analysis Purified Cystatin A Mouse Monoclonal Antibody (CSTA/3553). Confirmation of Purity and Integrity of Antibody)

IHC (Immunohiostchemistry)

(Formalin-fixed, paraffin-embedded human Prostate stained with Cystatin A Mouse Monoclonal Antibody (CSTA/3553).)

IHC (Immunohistochemistry)

(Formalin-fixed, paraffin-embedded human Tonsil stained with Cystatin A Mouse Monoclonal Antibody (CSTA/3553).)

References

Takahashi, H., et al. 2001. J. Biol. Chem. 276: 36632-36638.

NCBI and Uniprot Product Information

• NCBI GI #: 4885165
• NCBI GeneID: 1475
• NCBI Accession #: NP_005204.1
• NCBI GenBank Nucleotide #: NP_005204.1
• UniProt Accession #: P01040
• Molecular Weight: 11kDa
• NCBI Official Full Name: cystatin-A
• NCBI Official Synonym Full Names: cystatin A
• NCBI Official Symbol: CSTA
• NCBI Official Synonym Symbols: AREI; PSS4; STF1; STFA
• NCBI Protein Information: cystatin-A
• UniProt Protein Name: Cystatin-A
• UniProt Gene Name: CSTA
• UniProt Synonym Gene Names: STF1; STFA
• UniProt Entry Name: CYTA_HUMAN