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Application Data
(Staining of human peripheral blood granulocytes with Mouse anti Human Cytochrome b-245 Light Chain. Permeabilised with Leucoperm)
Mouse CYTOCHROME B245 Monoclonal Antibody | anti-CYBA antibody
MOUSE ANTI HUMAN CYTOCHROME B245 LIGHT CHAIN
Gene Names
CYBA; p22-PHOX
Applications
Western Blot, Immunoprecipitation, Flow Cytometry, Functional Assay, ELISA, Immunohistochemistry
Synonyms
CYTOCHROME B245, Antibody; MOUSE ANTI HUMAN CYTOCHROME B245 LIGHT CHAIN; anti-CYBA antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG1
Clone Number
CS9
Form/Format
Purified
Purified IgG - liquid
Purified IgG - liquid
Concentration
IgG concentration 1.0mg/ml (varies by lot)
Sequence Length
195
Applicable Applications for anti-CYBA antibody
WB (Western Blot), IP (Immunoprecipitation), FCM/FACS (Flow Cytometry), ELISA, IHC (Immunohistochemistry)
Perservative Stabilisers
0.09% Sodium Azide (NaN3)
Preparation
Preparation
Immunogen
Cytochrome B245 solubilised in octyl-beta-gluycopyranoside
Fusion Partners
Fusion Partners
Target Species
Human
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Shelf Life: 18 months from date of despatch.
Application Data
(Staining of human peripheral blood granulocytes with Mouse anti Human Cytochrome b-245 Light Chain. Permeabilised with Leucoperm)
Application Data
(Staining of human peripheral blood granulocytes with Mouse anti Human Cytochrome b-245 Light Chain. Permeabilised with Leucoperm)
Application Data
(Staining of human peripheral blood granulocytes with Mouse anti Human Cytochrome b-245 Light Chain:Alexa Fluor ?647 (AAA49770A647). Permeabilised with Leucoperm)
Application Data
(Staining of human peripheral blood granulocytes with Mouse anti Human Cytochrome b-245 Light Chain:Alexa Fluor ?647 (AAA49770A647). Permeabilised with Leucoperm)
Application Data
(Staining of human peripheral blood granulocytes with Mouse anti Human Cytochrome b-245 Light Chain:FITC . Permeabilised with Leucoperm)
Application Data
(Staining of human peripheral blood granulocytes with Mouse anti Human Cytochrome b-245 Light Chain:FITC . Permeabilised with Leucoperm)
Application Data
(Staining of human peripheral blood granulocytes with Mouse anti Human Cytochrome b-245 Light Chain:Alexa Fluor ?488 (AAA49770A488). Permeabilised with Leucoperm)
Application Data
(Staining of human peripheral blood granulocytes with Mouse anti Human Cytochrome b-245 Light Chain:Alexa Fluor ?488 (AAA49770A488). Permeabilised with Leucoperm)
Related Product Information for anti-CYBA antibody
Mouse anti Human Cytochrome B245 Light Chain antibody, clone CS9 recognizes the light chain of cytochrome B245, also known as p22phox, a subunit of the nicotinamide adenine dinucleotide phosphate-oxidase (NADPH oxidase). NADPH oxidase is a membrane-bound enzyme complex in neutrophils that produces the superoxide anion (O2-) by transferring single electrons from NADPH inside the cell across the membrane and coupling them to molecular oxygen. Superoxide is used for the destruction of pathogens. Individuals with defects in NADPH oxidase suffer from chronic granulomatous disease, characterised by severe recurrent infections. The light chain (p22phox), along with the heavy chain (gp91phox) form cytochrome B245 (also known as flavocytochome b, or Cyt b), which is always membrane-resident. Upon the appropriate stimulus, other components of NADPH oxidase (p47phox, p40phox, p67phox and Rac2) rapidly translocate from the cytosol to the cell membrane to form the complete enzyme complex. Cytochrome B245 appears to serve as a scaffold for the assembly of the other cytosolic units of the oxidase and to provide a transmembrane pathway for electrons. The core region of the CS9 epitope is amino acids 165 - 169 (KKPSE), a cytosolic epitope on p22phox not accessible on intact neutrophils. Mouse anti Human Cytochrome B245 Light Chain antibody, clone CS9 also has an inhibitory effect on NADPH oxidase activation.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
21,013 Da
NCBI Official Full Name
cytochrome b-245 light chain
NCBI Official Synonym Full Names
cytochrome b-245, alpha polypeptide
NCBI Official Symbol
CYBA
NCBI Official Synonym Symbols
p22-PHOX
NCBI Protein Information
cytochrome b-245 light chain; cytochrome b light chain; cytochrome b(558) alpha chain; cytochrome b(558) alpha-subunit; cytochrome b, alpha polypeptide; cytochrome b558 subunit alpha; flavocytochrome b-558 alpha polypeptide; neutrophil cytochrome b 22 kDa polypeptide; p22 phagocyte B-cytochrome; p22phox; superoxide-generating NADPH oxidase light chain subunit
UniProt Protein Name
Cytochrome b-245 light chain
UniProt Gene Name
CYBA
UniProt Synonym Gene Names
p22phox
UniProt Entry Name
CY24A_HUMAN
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Product Notes
The CYBA cyba (Catalog #AAA49770) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's CYTOCHROME B245 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IP (Immunoprecipitation), FCM/FACS (Flow Cytometry), ELISA, IHC (Immunohistochemistry). Researchers should empirically determine the suitability of the CYBA cyba for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CYTOCHROME B245, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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