Mouse Cytochrome C Monoclonal Antibody | anti-CYCS antibody

Cytochrome C (Mitochondrial Marker) Mouse Monoclonal Antibody

Cat. Num. AAA62898

• Gene Names: CYCS; CYC; HCS; THC4
• Reactivity: Human, Mouse, Rat, Horse, Dog, Pigeon, Frog, Drosophila.; Others not known
• Applications: Immunohistochemistry, Western Blot, Immunofluorescence, Flow Cytometry, Functional Assay
• Synonyms: Cytochrome C, Antibody; Cytochrome C (Mitochondrial Marker) Mouse Monoclonal Antibody; CYC; CYCS; HCS; THC4; anti-CYCS antibody

• Host: Mouse
• Reactivity: Human, Mouse, Rat, Horse, Dog, Pigeon, Frog, Drosophila.; Others not known
• Clonality: Monoclonal
• Isotype: IgG2b, kappa
• Clone Number: SPM389
• Specificity: It recognizes an epitope within amino acids 93-104 of pigeon Cytochrome C, a well-characterized mobile electron transport protein that is essential to energy conversion in all aerobic organisms. In mammalian cells, this highly conserved protein is normally localized to the mitochondrial inter-membrane space. More recent studies have identified cytosolic cytochrome c as a factor necessary for activation of apoptosis. During apoptosis, cytochrome c is trans-located from the mitochondrial membrane to the cytosol, where it is required for activation of caspase-3 (CPP32). Overexpression of Bcl-2 has been shown to prevent the translocation of cytochrome c, thereby blocking the apoptotic process. Overexpression of Bax has been shown to induce the release of cytochrome c and to induce cell death. The release of cytochrome c from the mitochondria is thought to trigger an apoptotic cascade, whereby Apaf-1 binds to Apaf-3 (caspase-9) in a cytochrome c-dependent manner, leading to caspase-9 cleavage of caspase-3. This MAb recognizes total cytochrome C which includes both apocytochrome (i.e. cytochrome in the cytosol without heme attached) and holocytochrome (i.e cytochrome in the mitochondria with heme attached).
• Form/Format: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 1mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 105

• Applicable Applications for anti-CYCS antibody: IHC (Immunohistochemistry), WB (Western Blot), IF (Immunofluorescence), FCM/FACS (Flow Cytometry)
• Cellular Localization: Cytoplasmic
• Immunogen: Synthetic peptides corresponding to amino acid 1-80, 81-104 and 66-104 of pigeon cytochrome c
• Hu-Chromosome Location: 7p15.3
• Positive Control: K-562, HL-60, Jurkat, NIH3T3 or PC-3 cells. Liver or Cardiac muscle.
• Preparation and Storage: Antibody with azide - store at 2 to 8 degree C. Antibody without azide - store at -20 to -80 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

IHC (Immunohiostchemistry)

(Formalin-fixed, paraffin-embedded human Pancreas stained with Cytochrome C Monoclonal Antibody (SPM389).)

IHC (Immunohistochemistry)

(Formalin-fixed, paraffin-embedded human Salivary Tumor stained with Cytochrome C Monoclonal Antibody (SPM389).)

References

Goshorn SG, E Retzel, and R Jemmerson. Common Structural Features among Monoclonal Antibodies Binding the Same Antigenic Region of Cytochrome c. J Biol Chem 266:2134-2142 (1991).

NCBI and Uniprot Product Information

• NCBI GI #: 11128019
• NCBI GeneID: 54205
• NCBI Accession #: NP_061820.1
• NCBI GenBank Nucleotide #: NM_018947.5
• UniProt Accession #: P99999; P00001; Q6NUR2; Q6NX69; Q96BV4; A4D166; B2R4I1
• Molecular Weight: 15kDa
• NCBI Official Full Name: cytochrome c
• NCBI Official Synonym Full Names: cytochrome c, somatic
• NCBI Official Symbol: CYCS
• NCBI Official Synonym Symbols: CYC; HCS; THC4
• NCBI Protein Information: cytochrome c
• UniProt Protein Name: Cytochrome c
• UniProt Gene Name: CYCS
• UniProt Synonym Gene Names: CYC
• UniProt Entry Name: CYC_HUMAN