Mouse anti-Human Cytokeratin 15 Monoclonal Antibody | anti-KRT15 antibody

Cytokeratin 15 (Esophageal Squamous Cell Carcinoma Marker)

Cat. Num. AAA23924

• Gene Names: KRT15; K15; CK15; K1CO
• Reactivity: Human
• Applications: Immunofluorescence, Western Blot, Immunohistochemistry
• Purity: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Synonyms: Cytokeratin 15, Antibody; Cytokeratin 15 (Esophageal Squamous Cell Carcinoma Marker); CK15; Cytokeratin 15; K1CO; Ka15; Keratin 15 basic; Keratin 15 beta; Keratin complex 1 acidic gene 15; Keratin type I cytoskeletal 15; KRT15; KRTB; KRTL15; Type I cytoskeletal 15; Type I keratin Ka15; anti-KRT15 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2c, kappa
• Clone Number: KRT15/2959
• Specificity: Keratin 15 is a type I keratin which is expressed only in basal keratinocytes in stratified epithelia and does not appear to have a natural type II expression partner. Keratin 15 is down regulated in activated keratinocytes. Cytokeratin 15 is a specific marker of stem cells of the hair-follicle bulge and may be a useful marker for between basal cell carcinoma (BCC) and trichoepithelioma. Trichoblastoma are benign neoplasms of follicular differentiation frequently found in nevus sebaceous. Many morphologic features are shared with nodular basal cell carcinoma, sometimes rendering a difficult. Trichoblastoma and BCC show variable expression of Cytokeratin 15 and Cytokeratin 19, and absence of hair keratins.
• Purity/Purification: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Form/Format: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 456

• Applicable Applications for anti-KRT15 antibody: Immunofluorescence (IF), Western Blot (WB), Immunohistochemistry (IHC) Paraffin
• Application Notes: IF: 1-2ug/million cells; WB: 1-2ug/ml; Immunohistochemistry (Frozen & formalin-fixed) (1-2ug/ml for 30 min at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
• Positive Control: MCF-7, A431 cells. Skin or Colon or Thymus.
• Immunogen: Recombinant full-length human KRT15 protein
• Cellular Localization: Cytoplasmic
• Hu-chromosome Location: 17q21.2
• Preparation and Storage: Antibody with azide: store at 2 to 8 degree C.; Antibody without azide: store at -20 to -80 degree C.; Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Application Data

(Analysis of Protein Array containing more than 19,000 full-length human proteins using Cytokeratin 15 Mouse Monoclonal Antibody (KRT15/2959). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)

SDS-PAGE

(SDS-PAGE Analysis Purified Cytokeratin 15 Mouse Monoclonal Antibody (KRT15/2959). Confirmation of Purity and Integrity of Antibody)

WB (Western Blot)

(Western Blot Analysis of Human Thymus tissue lysate using Cytokeratin 15 Mouse Monoclonal Antibody (KRT15/2959))

IF (Immunofluorescence)

(Confocal immunofluorescence image of MCF-7 cells using Cytokeratin 15 Mouse Monoclonal Antibody (KRT15/2959) followed by Goat anti-Mouse CF488 (Cyan) and Reddot is used to label the nuclei Red.)

IHC (Immunohistochemistry)

(Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with Cytokeratin 15 Mouse Monoclonal Antibody (KRT15/2959).)

IHC (Immunohistochemistry)

(Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with Cytokeratin 15 Mouse Monoclonal Antibody (KRT15/2959))

References

Yu-Hong Shen, et al. Asian Pac J Cancer Prev, 17 (9), 4217-4222.

NCBI and Uniprot Product Information

• NCBI GI #: 24430190
• NCBI GeneID: 3866
• NCBI Accession #: NP_002266.2
• NCBI GenBank Nucleotide #: NP_002266.2
• UniProt Accession #: P19012
• Molecular Weight: 52kDa
• NCBI Official Full Name: keratin, type I cytoskeletal 15
• NCBI Official Synonym Full Names: keratin 15
• NCBI Official Symbol: KRT15
• NCBI Official Synonym Symbols: K15; CK15; K1CO
• NCBI Protein Information: keratin, type I cytoskeletal 15
• UniProt Protein Name: Keratin, type I cytoskeletal 15
• UniProt Gene Name: KRT15
• UniProt Synonym Gene Names: KRTB; CK-15; K15
• UniProt Entry Name: K1C15_HUMAN