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Application Data
(Analysis of Protein Array containing more than 19,000 full-length human proteins using Cytokeratin 15 Mouse Monoclonal Antibody (KRT15/2957). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)
Mouse anti-Human Cytokeratin 15 Monoclonal Antibody | anti-KRT15 antibody
Cytokeratin 15 (Esophageal Squamous Cell Carcinoma Marker)
Gene Names
KRT15; K15; CK15; K1CO
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Immunofluorescence, Western Blot, Immunohistochemistry
Purity
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Synonyms
Cytokeratin 15, Antibody; Cytokeratin 15 (Esophageal Squamous Cell Carcinoma Marker); CK15; Cytokeratin 15; K1CO; Ka15; Keratin 15 basic; Keratin 15 beta; Keratin complex 1 acidic gene 15; Keratin type I cytoskeletal 15; KRT15; KRTB; KRTL15; Type I cytoskeletal 15; Type I keratin Ka15; anti-KRT15 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2c, kappa
Clone Number
KRT15/2957
Specificity
Keratin 15 is a type I keratin which is expressed only in basal keratinocytes in stratified epithelia and does not appear to have a natural type II expression partner. Keratin 15 is down regulated in activated keratinocytes. Cytokeratin 15 is a specific marker of stem cells of the hair-follicle bulge and may be a useful marker for between basal cell carcinoma (BCC) and trichoepithelioma. Trichoblastoma are benign neoplasms of follicular differentiation frequently found in nevus sebaceous. Many morphologic features are shared with nodular basal cell carcinoma, sometimes rendering a difficult. Trichoblastoma and BCC show variable expression of Cytokeratin 15 and Cytokeratin 19, and absence of hair keratins.
Purity/Purification
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Form/Format
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Sequence Length
456
Applicable Applications for anti-KRT15 antibody
Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB), Immunohistochemistry (IHC) Paraffin
Application Notes
FC/FACS: 1-2ug/million cells
IF: 1-2ug/ml
WB: 1-2ug/ml
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
IF: 1-2ug/ml
WB: 1-2ug/ml
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
Positive Control
HCT116, HeLa, A431 cells. Skin or Colon.
Immunogen
Recombinant full-length human KRT15 protein
Cellular Localization
Cytoplasmic
Hu-chromosome Location
17q21.2
Preparation and Storage
Antibody with azide: store at 2 to 8 degree C.
Antibody without azide: store at -20 to -80 degree C.
Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Antibody without azide: store at -20 to -80 degree C.
Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Application Data
(Analysis of Protein Array containing more than 19,000 full-length human proteins using Cytokeratin 15 Mouse Monoclonal Antibody (KRT15/2957). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)
Application Data
(Analysis of Protein Array containing more than 19,000 full-length human proteins using Cytokeratin 15 Mouse Monoclonal Antibody (KRT15/2957). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)
WB (Western Blot)
(Western Blot Analysis of HCT116 cell lysate using Cytokeratin 15 Mouse Monoclonal Antibody (KRT15/2957))
WB (Western Blot)
(Western Blot Analysis of HCT116 cell lysate using Cytokeratin 15 Mouse Monoclonal Antibody (KRT15/2957))
FCM (Flow Cytometry)
(Flow Cytometric Analysis of PFA-fixed HeLa cells using Cytokeratin 15 Mouse MAb (KRT15/2957) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).)
FCM (Flow Cytometry)
(Flow Cytometric Analysis of PFA-fixed HeLa cells using Cytokeratin 15 Mouse MAb (KRT15/2957) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).)
SDS-PAGE
(SDS-PAGE Analysis Purified Cytokeratin 15 Mouse Monoclonal Antibody (KRT15/2957). Confirmation of Purity and Integrity of Antibody)
SDS-PAGE
(SDS-PAGE Analysis Purified Cytokeratin 15 Mouse Monoclonal Antibody (KRT15/2957). Confirmation of Purity and Integrity of Antibody)
WB (Western Blot)
(Western Blot Analysis of Human Thymus tissue lysate using Cytokeratin 15 Mouse Monoclonal Antibody (KRT15/2957))
WB (Western Blot)
(Western Blot Analysis of Human Thymus tissue lysate using Cytokeratin 15 Mouse Monoclonal Antibody (KRT15/2957))
IF (Immunofluorescence)
(Immunofluorescence Analysis of methanol-fixed human MCF-7 cells. Cytokeratin 15 Mouse Monoclonal Antibody (KRT15/2957) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Reddot (Red))
IF (Immunofluorescence)
(Immunofluorescence Analysis of methanol-fixed human MCF-7 cells. Cytokeratin 15 Mouse Monoclonal Antibody (KRT15/2957) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Reddot (Red))
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with Cytokeratin 15 Mouse Monoclonal Antibody (KRT15/2957))
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with Cytokeratin 15 Mouse Monoclonal Antibody (KRT15/2957))
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human Basal Cell Carcinoma stained with Cytokeratin 15 Mouse Monoclonal Antibody (KRT15/2957).)
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human Basal Cell Carcinoma stained with Cytokeratin 15 Mouse Monoclonal Antibody (KRT15/2957).)
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with Cytokeratin 15 Mouse Monoclonal Antibody (KRT15/2957))
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with Cytokeratin 15 Mouse Monoclonal Antibody (KRT15/2957))
References
Yu-Hong Shen, Cui-Ping Xu, Zhi-Meng Shi, Yan-Jiao Zhang, Ya-Guang Qiao, He-Ping Zhao. Cytokeratin 15 is an Effective Indicator for Progression and Malignancy of Esophageal Squamous Cell Carcinomas. Asian Pac J Cancer Prev, 17 (9), 4217-4222.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
52kDa
NCBI Official Full Name
keratin, type I cytoskeletal 15
NCBI Official Synonym Full Names
keratin 15
NCBI Official Symbol
KRT15
NCBI Official Synonym Symbols
K15; CK15; K1CO
NCBI Protein Information
keratin, type I cytoskeletal 15
UniProt Protein Name
Keratin, type I cytoskeletal 15
UniProt Gene Name
KRT15
UniProt Synonym Gene Names
KRTB; CK-15; K15
UniProt Entry Name
K1C15_HUMAN
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Product Notes
The KRT15 krt15 (Catalog #AAA23923) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Cytokeratin 15 (Esophageal Squamous Cell Carcinoma Marker) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Cytokeratin 15 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB), Immunohistochemistry (IHC) Paraffin. FC/FACS: 1-2ug/million cells IF: 1-2ug/ml WB: 1-2ug/ml Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes). Researchers should empirically determine the suitability of the KRT15 krt15 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Cytokeratin 15, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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