IHC (Immunohistochemistry)
(CK18 expression in the adenosquamous carcinoma of the human pancreas. Formalin fixed, paraffin embedded human tissue (4 ?m section) stained with anti - Cytokeratin 18 (AAA78992) monospecific clonal antibody according to related product)
Rabbit anti-Human Cytokeratin 18 Monoclonal Antibody | anti-KRT18 antibody
Anti - Cytokeratin 18
Gene Names
KRT18; K18; CYK18
Reactivity
Human
Applications
Immunohistochemistry
Synonyms
Cytokeratin 18, Antibody; Anti - Cytokeratin 18; anti-KRT18 antibody
Host
Rabbit
Reactivity
Human
Clonality
Monoclonal
Clone Number
R20-H
Specificity
Human
Form/Format
20 mM Tris-HCI, pH 8.0
Sequence Length
430
Applicable Applications for anti-KRT18 antibody
IHC (Immunohistochemistry)
Immunogen
Peptide derived from C-terminal region of human cytokeratin 18. Antibody recognizes the epitope between Arg412 - Arg429
Preservative
0.05% NaN3
Stabilizer
20 mg/ml BSA
Cellular localization
Cytoplasm
Positive control
Liver tissue
VENTANA PROTOCOL
INSTRUCTION MANUAL SHORT APPLICATION PROTOCOL FOR VENTANA BENCHMARK SLIDE STAINING SYSTEM PROCEDURE: U ultraView DAB
1. Deparafinization
2. Heating (72 °C) at the medium temperatures. Deparafinization.
3. Cell conditioning
4. ULTRA conditioner #1
5. Heating glass (95 °C), incubation 8 min. (Cell conditioner #1; buffer CC1).
6. ULTRA CC1 solution application – 36 min.
7. Antibody incubation temperature
8. Heating glass (36 °C), incubation 4 min.
9. Titration
10. Hand apply – primary antibody 100 ?l. Incubation 36 min.
11. ultraWash
12. Nuclear stain
13. Hematoxylin II application – one drop (nuclear stain). Cover and incubate 12 min.
14. After nuclear stain
15. Bluing reagent application, one drop. After nuclear stain, cover and incubate 4 min
1. Deparafinization
2. Heating (72 °C) at the medium temperatures. Deparafinization.
3. Cell conditioning
4. ULTRA conditioner #1
5. Heating glass (95 °C), incubation 8 min. (Cell conditioner #1; buffer CC1).
6. ULTRA CC1 solution application – 36 min.
7. Antibody incubation temperature
8. Heating glass (36 °C), incubation 4 min.
9. Titration
10. Hand apply – primary antibody 100 ?l. Incubation 36 min.
11. ultraWash
12. Nuclear stain
13. Hematoxylin II application – one drop (nuclear stain). Cover and incubate 12 min.
14. After nuclear stain
15. Bluing reagent application, one drop. After nuclear stain, cover and incubate 4 min
LEICA BOND MAX PROTOCOL
INSTRUCTION MANUAL SHORT APPLICATION PROTOCOL FOR LEICA BOND MAX SLIDE STAINING SYSTEM Protocol F:
Visualization system: BOND Refine DS9800
Epitope retrieval / heating time / temperature: ER2 / 30 min. / 100 °C
Incubation of primary antibody / temperature: 30 min. / 20 °C.
Visualization system: BOND Refine DS9800
Epitope retrieval / heating time / temperature: ER2 / 30 min. / 100 °C
Incubation of primary antibody / temperature: 30 min. / 20 °C.
PRECAUTIONS
1. We strongly recommend to use Primary Antibody Diluent (catalog number MBS684047), eventually alternative diluent (containing protease free BSA at the concentrations > 1mg/ml) for dilution of concentrated antibodies, otherwise the warranty might be voided.
2. Centrifuge the vial before use.
3. Intended for professional In Vitro TEST use in laboratories.
4. Do not use after expiration date stamped on vial label.
5. Avoid contamination of the reagent.
6. Any discrepancies in the recommended procedures stated in the working protocol may affect the final results.
7. The reagent contains sodium azide (NaN3) which is highly toxic in higher concentrations. The concentration in the reagent (0.05%) is not considered as hazardous.
8. Disposal of waste material must be conducted in accordance with local regulations.
9. Wear appropriate Personal Protective Equipment to avoid contact with eyes and skin.
2. Centrifuge the vial before use.
3. Intended for professional In Vitro TEST use in laboratories.
4. Do not use after expiration date stamped on vial label.
5. Avoid contamination of the reagent.
6. Any discrepancies in the recommended procedures stated in the working protocol may affect the final results.
7. The reagent contains sodium azide (NaN3) which is highly toxic in higher concentrations. The concentration in the reagent (0.05%) is not considered as hazardous.
8. Disposal of waste material must be conducted in accordance with local regulations.
9. Wear appropriate Personal Protective Equipment to avoid contact with eyes and skin.
Preparation and Storage
Store at +4°C.
IHC (Immunohistochemistry)
(CK18 expression in the adenosquamous carcinoma of the human pancreas. Formalin fixed, paraffin embedded human tissue (4 ?m section) stained with anti - Cytokeratin 18 (AAA78992) monospecific clonal antibody according to related product)
IHC (Immunohistochemistry)
(CK18 expression in the adenosquamous carcinoma of the human pancreas. Formalin fixed, paraffin embedded human tissue (4 ?m section) stained with anti - Cytokeratin 18 (AAA78992) monospecific clonal antibody according to related product)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
48,058 Da
NCBI Official Full Name
Keratin, type I cytoskeletal 18
NCBI Official Synonym Full Names
keratin 18
NCBI Official Symbol
KRT18
NCBI Official Synonym Symbols
K18; CYK18
NCBI Protein Information
keratin, type I cytoskeletal 18; CK-18; keratin-18; cytokeratin 18; cytokeratin-18; cell proliferation-inducing protein 46; cell proliferation-inducing gene 46 protein
UniProt Protein Name
Keratin, type I cytoskeletal 18
UniProt Gene Name
KRT18
UniProt Synonym Gene Names
CYK18; CK-18; K18
UniProt Entry Name
K1C18_HUMAN
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Product Notes
The KRT18 krt18 (Catalog #AAA78992) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti - Cytokeratin 18 reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Cytokeratin 18 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry). Researchers should empirically determine the suitability of the KRT18 krt18 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Cytokeratin 18, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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