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FCM (Flow Cytometry)
(Flow cytometric analysis of MCF-7 cells with Cytokeratin 18 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody)
Rabbit Cytokeratin 18 Monoclonal Antibody | anti-KRT18 antibody
Cytokeratin 18 Antibody
Gene Names
KRT18; K18; CYK18
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Flow Cytometry, Functional Assay
Purity
ProA affinity purified
Synonyms
Cytokeratin 18; Monoclonal Antibody; Cytokeratin 18 Antibody; Cell proliferation inducing gene 46 protein antibody; Cell proliferation inducing protein 46 antibody; Cell proliferation-inducing gene 46 protein antibody; CK 18 antibody; CK-18 antibody; CK18 antibody; CYK 18 antibody; CYK18 antibody; Cytokeratin 18 antibody; Cytokeratin endo B antibody; Cytokeratin-18 antibody; K 18 antibody; K18 antibody; K1C18_HUMAN antibody; KA18 antibody; Keratin 18 antibody; Keratin 18; type I antibody; Keratin D antibody; keratin; type I cytoskeletal 18 antibody; Keratin-18 antibody; Krt18 antibody; anti-KRT18 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Clone Number
SZ80-07
Purity/Purification
ProA affinity purified
Form/Format
1*TBS (pH7.4), 1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Applicable Applications for anti-KRT18 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes
WB: 1:10,000-1:50,000
IHC: 1:50-1:200
ICC: 1:100-1:500
FC/FACS: 1:50-1:100
IHC: 1:50-1:200
ICC: 1:100-1:500
FC/FACS: 1:50-1:100
Antibody Type
Recombinant Antibody
Immunogen
Recombinant protein
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C.
FCM (Flow Cytometry)
(Flow cytometric analysis of MCF-7 cells with Cytokeratin 18 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody)
FCM (Flow Cytometry)
(Flow cytometric analysis of MCF-7 cells with Cytokeratin 18 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody)
ICC (Immunocytochemistry)
(ICC staining Cytokeratin 18 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining Cytokeratin 18 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining Cytokeratin 18 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining Cytokeratin 18 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining Cytokeratin 18 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining Cytokeratin 18 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistchemistry)
(Immunohistochemical analysis of paraffin-embedded human gastric carcinoma tissue using anti-Cytokeratin 18 antibody. Counter stained with hematoxylin.)
IHC (Immunohistchemistry)
(Immunohistochemical analysis of paraffin-embedded human gastric carcinoma tissue using anti-Cytokeratin 18 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Cytokeratin 18 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Cytokeratin 18 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Cytokeratin 18 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Cytokeratin 18 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Cytokeratin 18 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Cytokeratin 18 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Cytokeratin 18 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Cytokeratin 18 antibody. Counter stained with hematoxylin.)
WB (Western Blot)
(Western blot analysis of Cytokeratin 18 on different lysates using anti-Cytokeratin 18 antibody at 1/20, 000 dilution. Positive control: Lane 1: A431 Lane 2: Mouse colon Lane 3: Mouse kidney)
WB (Western Blot)
(Western blot analysis of Cytokeratin 18 on different lysates using anti-Cytokeratin 18 antibody at 1/20, 000 dilution. Positive control: Lane 1: A431 Lane 2: Mouse colon Lane 3: Mouse kidney)
Related Product Information for anti-KRT18 antibody
Cytokeratins comprise a diverse group of intermediate filament proteins (IFPs) that are expressed as pairs in both keratinized and non-keratinized epithelial tissue. Cytokeratins play a critical role in differentiation and tissue specialization and function to maintain the overall structural integrity of epithelial cells. Cytokeratins have been found to be useful markers of tissue differentiation which is directly applicable to the characterization of malignant tumors. For example, Cytokeratins 10 and 13 are expressed highly in a subset of squamous cell carcinomas while Cytokeratin 18 is expressed in a majority of adenocarcinomas and basal cell carcinomas. Cytokeratin 18 contains two major phosphorylation sites on Ser 33 and Ser 52. Phosphorylation of Ser 18 is essential for the association of Cytokeratin 18 with 14-3-3 proteins and is involved in keratin organization and distribution.
Product Categories/Family for anti-KRT18 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
48,058 Da
NCBI Official Full Name
keratin, type I cytoskeletal 18
NCBI Official Synonym Full Names
keratin 18
NCBI Official Symbol
KRT18
NCBI Official Synonym Symbols
K18; CYK18
NCBI Protein Information
keratin, type I cytoskeletal 18; CK-18; keratin-18; cytokeratin 18; cytokeratin-18; cell proliferation-inducing protein 46; cell proliferation-inducing gene 46 protein
UniProt Protein Name
Keratin, type I cytoskeletal 18
UniProt Gene Name
KRT18
UniProt Synonym Gene Names
CYK18; CK-18; K18
UniProt Entry Name
K1C18_HUMAN
Similar Products
Product Notes
The KRT18 krt18 (Catalog #AAA30013) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Cytokeratin 18 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Cytokeratin 18 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). WB: 1:10,000-1:50,000 IHC: 1:50-1:200 ICC: 1:100-1:500 FC/FACS: 1:50-1:100. Researchers should empirically determine the suitability of the KRT18 krt18 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Cytokeratin 18, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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