WB (Western Blot)
(Western Blot; Sample: Lane1: Rat Liver lysate; Lane2: human Placenta lysate; Lane3: Rat Small intestine lysate; Lane4: Hela cell lysate Primary Ab: 2 ug/ml Mouse Anti-human KRT18 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)
Mouse anti-Human Cytokeratin 18 (CK18) Monoclonal Antibody | anti-CK18 antibody
Monoclonal Antibody to Cytokeratin 18 (CK18)
Gene Names
KRT18; K18; CYK18
Reactivity
Human
Applications
Immunoprecipitation, Immunocytochemistry, Immunohistochemistry, Western Blot
Purity
Protein A + Protein G affinity chromatography
Synonyms
Cytokeratin 18 (CK18), Antibody; Monoclonal Antibody to Cytokeratin 18 (CK18); Cytokeratin 18; KRT18; CYK18; K18; Keratin 18; Cell proliferation-inducing gene 46 protein; Keratin, type I cytoskeletal 18; anti-CK18 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1 Kappa
Clone Number
F1
Specificity
The antibody is a mouse monoclonal antibody raised against CK18. It has been selected for its ability to recognize CK18 in immunohistochemical staining and western blotting.
Purity/Purification
Protein A + Protein G affinity chromatography
Form/Format
Liquid. PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Concentration
1mg/mL (varies by lot)
Sequence Positions
Asp238~Leu396
Applicable Applications for anti-CK18 antibody
IP (Immunoprecipitation), ICC (Immunocytochemistry), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
Recombinant Cytokeratin 18 (CK18)
Preparation and Storage
Store at 4 degree C for frequent use. Store at -20 degree C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Stable for 12 months if correctly stored.
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Stable for 12 months if correctly stored.
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
WB (Western Blot)
(Western Blot; Sample: Lane1: Rat Liver lysate; Lane2: human Placenta lysate; Lane3: Rat Small intestine lysate; Lane4: Hela cell lysate Primary Ab: 2 ug/ml Mouse Anti-human KRT18 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)
WB (Western Blot)
(Western Blot; Sample: Lane1: Rat Liver lysate; Lane2: human Placenta lysate; Lane3: Rat Small intestine lysate; Lane4: Hela cell lysate Primary Ab: 2 ug/ml Mouse Anti-human KRT18 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)
IF (Immunofluorescence)
(FITC staining on IF; Sample: human HepG2 cell; Primary Ab: 30ug/ml Mouse Anti-human CK18 Antibody Second Ab: 2ug/ml FITC-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)
IF (Immunofluorescence)
(FITC staining on IF; Sample: human HepG2 cell; Primary Ab: 30ug/ml Mouse Anti-human CK18 Antibody Second Ab: 2ug/ml FITC-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)
Product Categories/Family for anti-CK18 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
48,058 Da
NCBI Official Full Name
keratin, type I cytoskeletal 18
NCBI Official Synonym Full Names
keratin 18
NCBI Official Symbol
KRT18
NCBI Official Synonym Symbols
K18; CYK18
NCBI Protein Information
keratin, type I cytoskeletal 18; CK-18; keratin-18; cytokeratin 18; cytokeratin-18; cell proliferation-inducing protein 46; cell proliferation-inducing gene 46 protein
UniProt Protein Name
Keratin, type I cytoskeletal 18
UniProt Gene Name
KRT18
UniProt Synonym Gene Names
CYK18; CK-18; K18
UniProt Entry Name
K1C18_HUMAN
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Product Notes
The CK18 krt18 (Catalog #AAA152764) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is Asp238~Leu396. The Monoclonal Antibody to Cytokeratin 18 (CK18) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Cytokeratin 18 (CK18) can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), ICC (Immunocytochemistry), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the CK18 krt18 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Cytokeratin 18 (CK18), Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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