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IF (Immunofluorescence)
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Mouse Cytokeratin 18 (KRT18) Monoclonal Antibody | anti-KRT18 antibody

Cytokeratin 18 (KRT18) Mouse Monoclonal Antibody

Gene Names
KRT18; K18; CK-18; CYK18
Reactivity
Human.
Does not react with Mouse, Rat, Sheep, Hamster, Cow, Dog and Pig
Applications
Flow Cytometry, Functional Assay, Immunofluorescence, Immunohistochemistry
Synonyms
Cytokeratin 18 (KRT18), Antibody; Cytokeratin 18 (KRT18) Mouse Monoclonal Antibody; Cell Proliferation-inducing Gene 46 Protein; CK18; CYK18 Cytokeratin Endo B; K18; Keratin-18; Kerd; KRT18; anti-KRT18 antibody
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For Research Use Only!
Host
Mouse
Reactivity
Human.
Does not react with Mouse, Rat, Sheep, Hamster, Cow, Dog and Pig
Clonality
Monoclonal
Isotype
IgG1, kappa
Clone Number
KRT18/834
Specificity
This MAb reacts with a wide variety of simple epithelia. It does not react with stratified squamous epithelia. It reacts with epithelial tumors of the gastrointestinal tract, lung, breast, pancreas, ovary, and thyroid. Cytokeratin 18, which belongs to the type A (acidic) subfamily of low molecular weight keratins, exists in combination with cytokeratin 8. It is reported that tissues from gastrointestinal tract are positive for both cytokeratin 8 and 18 but do not contain cytokeratin 14. Tissues from gastrointestinal tract, respiratory tract and urogenital tract, as well as endocrine and exocrine tissues and mesothelial cells are positive for cytokeratin 18.
Form/Format
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Sequence Length
430
Applicable Applications for anti-KRT18 antibody
Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC) Formalin
Application Notes
Western Blot (1-2ug/ml)
Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at RT)(Stainingof formalin-fixed tissues requires heating tissue sections in 10mM Tris with1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20minutes)Optimal dilution for a specific application should be determined.
Cellular Localization
Cytoplasmic
Immunogen
Recombinant full-length human KRT18 protein
Hu-Chromosome Location
12q13.13
Positive Control
MCF-7, HeLa, A431 cells or Breast Cancer
Preparation and Storage
Antibody without azide - store at -20 to -80 degree C. No MSDS required.

IF (Immunofluorescence)

FCM (Flow Cytometry)

WB (Western Blot)

WB (Western Blot)

WB (Western Blot)

IHC (Immunohistochemistry)

(Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with Cytokeratin 18 Monoclonal Antibody (KRT18/834).)

IHC (Immunohistchemistry)

(Formalin-fixed, paraffin-embedded human Bladder Carcinoma stained with Cytokeratin 18 Monoclonal Antibody (KRT18/834).)

IHC (Immunohistochemistry)

(Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with Cytokeratin 18 Monoclonal Antibody (KRT18/834).)

IHC (Immunohistochemistry)

(Formalin-fixed, paraffin-embedded human Lung Carcinoma stained with Cytokeratin 18 Monoclonal Antibody (KRT18/834).)

IHC (Immunohistochemistry)

(Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with Cytokeratin 18 Monoclonal Antibody (KRT18/834).)

IHC (Immunohistochemistry)

(Formalin-fixed, paraffin-embedded human Thyroid Carcinoma stained with Cytokeratin 18 Monoclonal Antibody (KRT18/834).)

IHC (Immunohistochemistry)

(Formalin-fixed, paraffin-embedded human Skin stained with Cytokeratin 18 Monoclonal Antibody (KRT18/834).)

References
Lauerova, L., et al. 1988. Novel monoclonal antibodies defining epitope of human cytokeratin 18 molecule. Hybridoma 5: 495-504

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
45kDa
NCBI Official Full Name
keratin, type I cytoskeletal 18
NCBI Official Synonym Full Names
keratin 18
NCBI Official Symbol
KRT18
NCBI Official Synonym Symbols
K18; CK-18; CYK18
NCBI Protein Information
keratin, type I cytoskeletal 18
UniProt Protein Name
Keratin, type I cytoskeletal 18
UniProt Gene Name
KRT18
UniProt Synonym Gene Names
CYK18; CK-18; K18
UniProt Entry Name
K1C18_HUMAN

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Product Notes

The KRT18 krt18 (Catalog #AAA13836) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Cytokeratin 18 (KRT18) Mouse Monoclonal Antibody reacts with Human. Does not react with Mouse, Rat, Sheep, Hamster, Cow, Dog and Pig and may cross-react with other species as described in the data sheet. AAA Biotech's Cytokeratin 18 (KRT18) can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC) Formalin. Western Blot (1-2ug/ml) Flow Cytometry (1-2ug/million cells) Immunofluorescence (1-2ug/ml) Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at RT)(Stainingof formalin-fixed tissues requires heating tissue sections in 10mM Tris with1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20minutes)Optimal dilution for a specific application should be determined. Researchers should empirically determine the suitability of the KRT18 krt18 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Cytokeratin 18 (KRT18), Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.