Mouse Cytokeratin 19 (KRT19) Monoclonal Antibody | anti-KRT19 antibody

Cytokeratin 19 (KRT19) (Pancreatic Stem Cell Marker) Mouse Monoclonal Antibody

Cat. Num. AAA13824

• Gene Names: KRT19; K19; CK19; K1CS
• Reactivity: Human, Mouse, Rat
• Applications: Flow Cytometry, Functional Assay, Immunofluorescence, Immunohistochemistry
• Synonyms: Cytokeratin 19 (KRT19), Antibody; Cytokeratin 19 (KRT19) (Pancreatic Stem Cell Marker) Mouse Monoclonal Antibody; k19; k1cs; Keratin 19 Keratin Type i 40kD; KRT19; anti-KRT19 antibody

• Host: Mouse
• Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Clone Number: KRT19/800
• Specificity: Recognizes a protein of 40kDa, identified as cytokeratin-19 (CK19), which is expressed in sweat gland, mammary gland ductal and secretory cells, bile ducts, gastrointestinal tract, bladder urothelium, oral epithelia, esophagus, and ectocervical epithelium. Anti-CK19 reacts with a wide variety of epithelial malignancies including adenocarcinomas of the colon, stomach, pancreas, biliary tract, liver, and breast. Perhaps the most useful application is the identification of thyroid carcinoma of the papillary type, although 50%-60% of follicular carcinomas are also labeled. Anti-CK19 is a useful marker for detection of tumor cells in lymph nodes, peripheral blood, bone marrow and breast cancer.
• Form/Format: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 400

• Applicable Applications for anti-KRT19 antibody: Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC) Formalin
• Application Notes: Flow Cytometry (0.5-1ug/million cells in 0.1ml); Immunofluorescence (1-2ug/ml); Immunohistochemistry (Formalin-fixed) (0.5-1ug/ml for 30 min at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
• Cellular Localization: Cytoplasmic
• Immunogen: Recombinant human KRT19 protein
• Hu-Chromosome Location: 17q21.2
• Positive Control: MCF-7, HeLa cells, Breast Cancer
• Preparation and Storage: Antibody with azide - store at 2 to 8 degree C. Antibody without azide - store at -20 to -80 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

IHC (Immunohistochemistry)

(Formalin-fixed, paraffin-embedded human Pancreas stained with Cytokeratin 19 Monoclonal Antibody (KRT19/800))

IHC (Immunohistochemistry)

(Formalin-fixed, paraffin-embedded Rat Lung stained with Cytokeratin 19 Monoclonal Antibody (KRT19/800))

IHC (Immunohistchemistry)

(Formalin-fixed, paraffin-embedded Rat Colon stained with Cytokeratin 19 Monoclonal Antibody (KRT19/800))

IHC (Immunohistochemistry)

(Formalin-fixed, paraffin-embedded Rat Stomach stained with Cytokeratin 19 Monoclonal Antibody (KRT19/800))

IHC (Immunohistochemistry)

(Formalin-fixed, paraffin-embedded human Bladder Carcinoma stained with Cytokeratin 19 Monoclonal Antibody (KRT19/800))

IHC (Immunohistochemistry)

(Formalin-fixed, paraffin-embedded human Cervical Carcinoma stained with Cytokeratin 19 Monoclonal Antibody (KRT19/800))

IHC (Immunohistochemistry)

(Formalin-fixed, paraffin-embedded human Endometrial Carcinoma stained with Cytokeratin 19 Monoclonal Antibody (KRT19/800))

IHC (Immunohistochemistry)

(Formalin-fixed, paraffin-embedded human Ovarian Carcinoma stained with Cytokeratin 19 Monoclonal Antibody (KRT19/800))

References

Van Eyken, P., et al. 1991. Immunocytochemistry of cytokeratins in primary human liver tumors. APMIS Suppl. 23: 77-85. van der Velden, L.A., et al. 1993. Cytokeratin expression in normal and (pre)malignant head and neck epithelia: an overview. Head Neck 15: 133-146.

NCBI and Uniprot Product Information

• NCBI GI #: 24234699
• NCBI GeneID: 3880
• NCBI Accession #: NP_002267.2
• NCBI GenBank Nucleotide #: NM_002276.4
• UniProt Accession #: P08727; Q5XG83; Q6NW33; Q7L5M9; Q96A53; Q96FV1; Q9BYF9; Q9P1Y4; B2R874
• Molecular Weight: 40kDa
• NCBI Official Full Name: keratin, type I cytoskeletal 19
• NCBI Official Synonym Full Names: keratin 19
• NCBI Official Symbol: KRT19
• NCBI Official Synonym Symbols: K19; CK19; K1CS
• NCBI Protein Information: keratin, type I cytoskeletal 19
• UniProt Protein Name: Keratin, type I cytoskeletal 19
• UniProt Gene Name: KRT19
• UniProt Synonym Gene Names: CK-19; K19
• UniProt Entry Name: K1C19_HUMAN