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Application Data
(Analysis of Protein Array containing more than 19,000 full-length human proteins using Cytokeratin 4 Mouse Monoclonal Antibody (KRT4/2804). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)
Mouse anti-Human Cytokeratin 4 (KRT4) Monoclonal Antibody | anti-KRT4 antibody
Cytokeratin 4 (KRT4)
Gene Names
KRT4; K4; CK4; CK-4; CYK4; WSN1
Reactivity
Human
Applications
Immunohistochemistry, Immunofluorescence, Flow Cytometry, Functional Assay
Purity
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Synonyms
Cytokeratin 4 (KRT4), Antibody; Cytokeratin 4 (KRT4); CK4; CYK4; Cytokeratin 4; Keratin 4; Keratin type II cytoskeletal 4; KRT4; anti-KRT4 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1, kappa
Clone Number
KRT4/2804
Specificity
Cytokeratin 4 (KRT4) is an intermediate filament protein associated with Cytokeratin 13 (KRT13). It is expressed in suprabasal cells of non-keratinized stratified squamous epithelium of esophagus, cornea, anus, larynx, pharynx and tongue. Decreased expression of KRT4 is associated with head and neck squamous carcinoma. It is helpful in differentiation of squamous cell carcinoma of esophagus origin from that of thyroid origin.
Purity/Purification
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Form/Format
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Sequence Length
534
Applicable Applications for anti-KRT4 antibody
IHC (Immunohistochemistry), IF (Immunofluorescence), FCM/FACS (Flow Cytometry)
Positive Control
HeLa, A549 or A431 cells. Skin, Cervix or Esophagus.
Immunogen
Recombinant fragment (around aa 181-292) of human KRT4 protein (exact sequence is proprietary)
Cellular Localization
Cytoplasmic and Cell Surface
Hu-chromosome Location
12q13.13
Preparation and Storage
Antibody with azide: store at 2 to 8 degree C.
Antibody without azide: store at -20 to -80 degree C.
Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Antibody without azide: store at -20 to -80 degree C.
Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Application Data
(Analysis of Protein Array containing more than 19,000 full-length human proteins using Cytokeratin 4 Mouse Monoclonal Antibody (KRT4/2804). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)
Application Data
(Analysis of Protein Array containing more than 19,000 full-length human proteins using Cytokeratin 4 Mouse Monoclonal Antibody (KRT4/2804). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)
IF (Immunofluorescence)
(Immunofluorescence Analysis of A549 cells labeling KRT4 with Cytokeratin 4 Mouse Monoclonal Antibody (KRT4/2804)followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Reddot (Red).)
IF (Immunofluorescence)
(Immunofluorescence Analysis of A549 cells labeling KRT4 with Cytokeratin 4 Mouse Monoclonal Antibody (KRT4/2804)followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Reddot (Red).)
FCM/FACS (Flow Cytometry)
(Flow Cytometric Analysis of HeLa cells using Cytokeratin 4 Mouse Monoclonal Antibody (KRT4/2804)followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).)
FCM/FACS (Flow Cytometry)
(Flow Cytometric Analysis of HeLa cells using Cytokeratin 4 Mouse Monoclonal Antibody (KRT4/2804)followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).)
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human Skin stained with Cytokeratin 4 Mouse Monoclonal Antibody (KRT4/2804).)
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human Skin stained with Cytokeratin 4 Mouse Monoclonal Antibody (KRT4/2804).)
References
Opitz Og, et al. J Biol Chem. 1998 Sep 11;273(37):23912-21.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
67kDa
NCBI Official Full Name
Keratin, type II cytoskeletal 4
NCBI Official Synonym Full Names
keratin 4
NCBI Official Symbol
KRT4
NCBI Official Synonym Symbols
K4; CK4; CK-4; CYK4; WSN1
NCBI Protein Information
keratin, type II cytoskeletal 4
UniProt Protein Name
Keratin, type II cytoskeletal 4
UniProt Gene Name
KRT4
UniProt Synonym Gene Names
CYK4; CK-4; K4
UniProt Entry Name
K2C4_HUMAN
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Product Notes
The KRT4 krt4 (Catalog #AAA215056) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Cytokeratin 4 (KRT4) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Cytokeratin 4 (KRT4) can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IF (Immunofluorescence), FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the KRT4 krt4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Cytokeratin 4 (KRT4), Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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