Mouse anti-Human CytokeRatin 7 / KeRatin K7 Monoclonal Antibody

Mouse anti CytokeRatin 7 / KeRatin K7

Cat. Num. AAA77462

• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, Immunohistochemistry, Immunocytochemistry, Flow Cytometry, Functional Assay
• Synonyms: CytokeRatin 7 / KeRatin K7, Antibody; Mouse anti CytokeRatin 7 / KeRatin K7; anti-CytokeRatin 7 / KeRatin K7 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: OVTL12/30
• Specificity: OVTL12/30 reacts exlcusively with cytokeRatin 7 which is present in specific glandular-type epithelia and most carcinomas derived thereof.
• Form/Format: Each vial contains 100 ul 1 mg/ml purified monoclonal antibody in PBS containing 0.09% sodium azide.

• Applicable Applications for anti-CytokeRatin 7 / KeRatin K7 antibody: WB (Western Blot), IHC (Immunohistochemistry), IHC (Immunohistochemistry), ICC (Immunocytochemistry), FCM/FACS (Flow Cytometry)
• Source Note: OVTL12/30 is a Mouse monoclonal IgG1/kappa antibody derived by fusion of SP2/0-Ag14 Mouse myeloma cells with spleen cells from a Mouse immunized with a cytoskeletal prepaRation of OTN-11 ovarian carcinoma cell line.
• Preparation and Storage: Store at 4 degree C, or in small aliquots at -20 degree C.

IHC (Immunohistochemisry)

(Figure 4: Frozen section of human kidney tissue immunostained showing strong reactivity in the epithelial cells of the tubules.)

IHC (Immunohiostchemistry)

(Figure 3. Immunohistochemistry on paraffin section of human breast. Positive staining of lobuli and ducti.)

IHC (Immunohistochemistry)

(Figure 2. Immunohistochemistry on paraffin section of human liver. Bile ducts are strongly positive.)

Related Product Information for anti-CytokeRatin 7 / KeRatin K7 antibody

CytokeRatins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in Human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9 - 7.8. The individual Human cytokeRatins are numbered 1 to 20. The various epithelia in the Human body usually express cytokeRatins which are not only characteristic of the type of epithelium, but also related to the degree of matuRation or differentiation within an epithelium. CytokeRatin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeRatin antibodies are not only of assistance in the differential identification of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.

Product Categories/Family for anti-CytokeRatin 7 / KeRatin K7 antibody

Cytoskeletal; Cancer; Cell differentiation; Cytoskeleton; Stem cell research

References

Ramaekers, F., van Niekerk, C., Poels, L., Schaafsma, E., Huijsmans, A., Robben, H., Schaart, G., and Vooijs, P. (1990). Use of monoclonal antibodies to keRatin 7 in the differential diagnosis of adenocarcinomas, Am J Pathol 136, 641-55. van Niekerk, C. C., Jap, P. H., Ramaekers, F. C., van de Molengraft, F., and Poels, L. G. (1991). Immunohistochemical demonstRation of keRatin 7 in routinely fixed paraffin-embedded Human tissues, J Pathol 165, 145-52. van Niekerk, C. C., Boerman, O. C., Ramaekers, F. C., and Poels, L. G. (1991). Marker profile of different phases in the transition of normal Human ovarian epithelium to ovarian carcinomas, Am J Pathol 138, 455-63. van de Molengraft, F. J., van Niekerk, C. C., Jap, P. H., and Poels, L. G. (1993). OV-TL 12/30 (keRatin 7 antibody) is a marker of glandular differentiation in lung cancer, Histopathology 22, 35-8. van Niekerk, C. C., Vooijs, P., Casparie-van Velsen, I. J., and Poels, L. G. (1997). Differentiation margins of ovarian tumor pathology: first incidences of epithelial ovarian tumors monitored by marker antibodies, Cancer Detect Prev 21, 247-57. Torenbeek, R., Lagendijk, J. H., Van Diest, P. J., Bril, H., van de Molengraft, F. J., and Meijer, C. J. (1998). Value of a panel of antibodies to identify the primary origin of adenocarcinomas presenting as bladder carcinoma, Histopathology 32, 20-7.