WB (Western Blot)
( Western Blot using anti-DARC antibody 2C3 Human liver lysate samples (35ug protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of 2C3 at 0.03 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected running size for DARC is 35.6kDa. successfully detected DARC in human liver lysate.)
DARC recombinant antibody
Anti-DARC [2C3]
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, ELISA
Purity
Protein A Affinity Purified
Synonyms
DARC, Recombinant Antibody; Anti-DARC [2C3]; Duffy Antigen Receptor for Chemokines; Fy6; NaM185-2C3; DARC recombinant antibody
Reactivity
Human
Clonality
Monoclonal
Isotype
Rabbit IgG, Kappa
Clone Number
2C3
Specificity
The antibody binds specifcally to the pentapeptide FEDVW of the Duffy receptor.
Purity/Purification
Protein A Affinity Purified
Concentration
1 mg/ml (varies by lot)
Sequence Length
337
Applicable Applications for DARC recombinant antibody
Blocking, FCM/FACS (Flow Cytometry), ELISA
Immunogen
CHO cells transfected with cDNA encoding the Duffy glycoprotein.
Buffer Composition
PBS only with 0.02% Proclin 300.
Chimeric Use Statement
This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Labeled Substance
None
Preparation and Storage
Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.
WB (Western Blot)
( Western Blot using anti-DARC antibody 2C3 Human liver lysate samples (35ug protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of 2C3 at 0.03 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected running size for DARC is 35.6kDa. successfully detected DARC in human liver lysate.)
WB (Western Blot)
( Western Blot using anti-DARC antibody 2C3 Human liver lysate samples (35ug protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of 2C3 at 0.03 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected running size for DARC is 35.6kDa. successfully detected DARC in human liver lysate.)
IF (Immunofluorescence)
( Immunofluorescence staining of fixed HepG2 cells with anti-DARC antibody 2C3 Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells on Shi-fix coverslips, permeabilized with 0.15% Triton and stained with the chimeric rabbit IgG version of 2C3 at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing cytoplasmic staining, likely of endosomes, and some membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor 488 secondary antibody.)
IF (Immunofluorescence)
( Immunofluorescence staining of fixed HepG2 cells with anti-DARC antibody 2C3 Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells on Shi-fix coverslips, permeabilized with 0.15% Triton and stained with the chimeric rabbit IgG version of 2C3 at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing cytoplasmic staining, likely of endosomes, and some membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor 488 secondary antibody.)
Related Product Information for DARC recombinant antibody
Recombinant monoclonal antibody to DARC. Manufactured using a proprietary Recombinant Platform with variable regions (i.e. specificity) from the hybridoma 2C3.
CHO cells transfected with cDNA encoding the Duffy glycoprotein.
CHO cells transfected with cDNA encoding the Duffy glycoprotein.
Product Categories/Family for DARC recombinant antibody
References
Wasniowska et al. Structural characterization of the epitope recognized by the new anti-Fy6 monoclonal antibody NaM 185-2C3. Transfusion Medicine 2002; 12(3):204-211 PMID:12164140
NCBI and Uniprot Product Information
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Product Notes
The DARC darc (Catalog #AAA72013) is a Recombinant Antibody and is intended for research purposes only. The product is available for immediate purchase. The Anti-DARC [2C3] reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's DARC can be used in a range of immunoassay formats including, but not limited to, Blocking, FCM/FACS (Flow Cytometry), ELISA. Researchers should empirically determine the suitability of the DARC darc for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DARC, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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