Rabbit anti-Human DAXX Monoclonal Antibody | anti-DAXX antibody

DAXX Antibody

Cat. Num. AAA312270

• Gene Names: DAXX; DAP6; EAP1; BING2
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunocytochemistry, Western Blot
• Purity: ProA affinity purified
• Synonyms: DAXX, Antibody; DAXX Antibody; BING 2 antibody; BING2 antibody; CENP-C binding protein antibody; DAP 6 antibody; DAP6 antibody; DAXX antibody; DAXX_HUMAN antibody; Death associated protein 6 antibody; Death domain associated protein 6 antibody; Death domain associated protein antibody; Death domain-associated protein 6 antibody; EAP 1 antibody; EAP1 antibody; ETS1 associated protein 1 antibody; ETS1-associated protein 1 antibody; Fas binding protein antibody; Fas death domain associated protein antibody; Fas death domain-associated protein antibody; hDaxx antibody; MGC126245 antibody; MGC126246 antibody; anti-DAXX antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Monoclonal
• Clone Number: SC54-06
• Purity/Purification: ProA affinity purified
• Form/Format: 1*TBS (pH7.4), 1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

• Applicable Applications for anti-DAXX antibody: FCM/FACS (Flow Cytometry), ICC (Immunocytochemistry), WB (Western Blot)
• Antibody Type: Recombinant Antibody
• Immunogen: Recombinant protein
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20 degree C.

FCM/FACS (Flow Cytometry)

(Flow cytometric analysis of Hela cells with Daxx antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody)

ICC (Immunocytochemistry)

(ICC staining Daxx in PANC-1 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry)

(ICC staining Daxx in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

WB (Western Blot)

(Western blot analysis of Daxx on different lysates using anti-Daxx antibody at 1/1, 000 dilution. Positive control: Lane 1: Hela Lane 2: A549 Lane 3: SW480)

Related Product Information for anti-DAXX antibody

Activation of the cell surface receptor FAS by FAS ligand leads to the initiation of apoptosis, a process necessary for the regulation of the immune system and tissue homeostasis. FAS-mediated apoptosis appears to involve a number of divergent and overlapping pathways. Daxx appears to be a central component of a FAS-mediated apoptotic pathway involving the activation of Jun N-terminal kinase (JNK). Although Daxx itself does not contain a death domain, it specifically binds to the death domain of FAS. Overexpression of Daxx activates the JNK pathway and enhances FAS-mediated apoptosis. The Daxx apoptotic pathway acts cooperatively with but is distinct from the FAS-mediated pathway that involves interactions between the death domain-containing protein FADD and the cysteine protease FLICE. Unlike the FAS-FADD-FLICE pathway, the Daxx pathway is sensitive to the apoptotic inhibitor protein Bcl-2.

Product Categories/Family for anti-DAXX antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 215422388
• NCBI GeneID: 1616
• NCBI Accession #: NP_001135441.1
• NCBI GenBank Nucleotide #: NM_001141969.1
• UniProt Accession #: Q9UER7; O14747; O15141; O15208; Q5STK9; Q9BWI3; B4E1I3; F5H082
• Molecular Weight: 81,373 Da
• NCBI Official Full Name: death domain-associated protein 6 isoform a
• NCBI Official Synonym Full Names: death-domain associated protein
• NCBI Official Symbol: DAXX
• NCBI Official Synonym Symbols: DAP6; EAP1; BING2
• NCBI Protein Information: death domain-associated protein 6; Fas-binding protein; CENP-C binding protein; ETS1-associated protein 1; death-associated protein 6; fas death domain-associated protein
• UniProt Protein Name: Death domain-associated protein 6
• UniProt Gene Name: DAXX
• UniProt Synonym Gene Names: BING2; DAP6; hDaxx; EAP1
• UniProt Entry Name: DAXX_HUMAN