WB (Western Blot)
(The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human DHFR antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : HeLa cell lysate Lane 2. : Jurkat cell lysate Lane 3 : 293T cell lysate)
Mouse anti-Human DHFR Monoclonal Antibody | anti-DHFR antibody
DHFR antibody
Gene Names
DHFR; DYR; DHFRP1
Reactivity
Human
Applications
Immunofluorescence, Immunocytochemistry, Flow Cytometry, Functional Assay, Western Blot, ELISA
Purity
By protein-A affinity chromatography
Synonyms
DHFR, Antibody; DHFR antibody; Dihydrofolate reductase; EC 1.5.1.3; anti-DHFR antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1, k
Clone Number
AT5B2
Purity/Purification
By protein-A affinity chromatography
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Concentration
1 mg/ml (varies by lot)
Sequence Length
187
Applicable Applications for anti-DHFR antibody
IF (Immunofluorescence), ICC (Immunocytochemistry), FCM/FACS (Flow Cytometry), WB (Western Blot), ELISA
Antigen Species
Human
Immunogen
Recombinant human DHFR (1-187aa) purified from E. coli
Clone Note
Anti-human DHFR mAb, clone AT5B2, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human DHFR protein.
Preparation and Storage
Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
WB (Western Blot)
(The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human DHFR antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : HeLa cell lysate Lane 2. : Jurkat cell lysate Lane 3 : 293T cell lysate)
WB (Western Blot)
(The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human DHFR antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : HeLa cell lysate Lane 2. : Jurkat cell lysate Lane 3 : 293T cell lysate)
Related Product Information for anti-DHFR antibody
Dihydrofolate reductase (DHFR) catalyzes the NADPH-dependent reduction of dihydrofolate to tetrahydrofolate, and is a crucial enzyme for the synthesis of purines, pyrimidines and some amino acids. Inhibition of the activity of this enzyme leads to arrest of DNA synthesis and cell death. Gene expression of methotrexate (MTX)-resistant variants of DHFR in normal hematopoietic cells is a potential strategy to permit administration of larger doses of MTX by alleviating drug toxicity in normal cells and tissues that are drug sensitive.
References
Chen MJ., et al. (1984) J Biol Chem. 259 (6): 3933-3943. ; Cody V., et al. (2009) Biochemistry. 48 (8): 1702-1711.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
15,672 Da
NCBI Official Full Name
dihydrofolate reductase isoform 1
NCBI Official Synonym Full Names
dihydrofolate reductase
NCBI Official Symbol
DHFR
NCBI Official Synonym Symbols
DYR; DHFRP1
NCBI Protein Information
dihydrofolate reductase
UniProt Protein Name
Dihydrofolate reductase
UniProt Gene Name
DHFR
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Product Notes
The DHFR dhfr (Catalog #AAA48167) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The DHFR antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's DHFR can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), ICC (Immunocytochemistry), FCM/FACS (Flow Cytometry), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the DHFR dhfr for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DHFR, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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