Application Data
(Immunoprecipitation analysis of Hela cell lysates using DLAT antibody.)
Mouse anti-Human, Mouse DLAT Monoclonal Antibody | anti-DLAT antibody
DLAT (Pyruvate Dehydrogenase E2) Antibody
Gene Names
DLAT; DLTA; PDCE2; PDC-E2
Reactivity
Human, Mouse
Applications
Immunoprecipitation, Immunocytochemistry, Western Blot
Purity
Affinity purified
Synonyms
DLAT, Antibody; DLAT (Pyruvate Dehydrogenase E2) Antibody; dihydrolipoamide S-acetyltransferase; PDC-E2; PDCE2; Pyruvate dehydrogenase complex component E2; DLTA; PBC; M2 antigen complex 70 kDa subunit; anti-DLAT antibody
Host
Mouse
Reactivity
Human, Mouse
Clonality
Monoclonal
Isotype
IgG1
Specificity
This antibody detects endogenous levels of DLAT and does not cross-react with related proteins.
Purity/Purification
Affinity purified
Form/Format
Purified in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.02% sodium azide, 50%, glycerol
Sequence Length
647
Applicable Applications for anti-DLAT antibody
IP (Immunoprecipitation), ICC (Immunocytochemistry), WB (Western Blot)
Immunogen
Purified recombinant human DLAT protein fragments expressed in E Coli.
Cellular Localization
Mitochondrion matrix
Application Data
(Immunoprecipitation analysis of Hela cell lysates using DLAT antibody.)
Application Data
(Immunoprecipitation analysis of Hela cell lysates using DLAT antibody.)
Application Data
(Western blot detection of DLAT in Jurkat, A549, U251, F9,LNCAP&Hela cell lysates using DLAT antibody (1:1000 diluted). Predicted band size:69KDa. Observed band size:69KDa.)
Application Data
(Western blot detection of DLAT in Jurkat, A549, U251, F9,LNCAP&Hela cell lysates using DLAT antibody (1:1000 diluted). Predicted band size:69KDa. Observed band size:69KDa.)
Application Data
(Immunocytochemistry stain of Hela using DLAT antibody (1:300).)
Application Data
(Immunocytochemistry stain of Hela using DLAT antibody (1:300).)
Related Product Information for anti-DLAT antibody
UniPort Summary Function: he pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO2. It contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3).
Entrez Summary: This gene encodes component E2 of the multi-enzyme pyruvate dehydrogenase complex (PDC). PDC resides in the inner mitochondrial membrane and catalyzes the conversion of pyruvate to acetyl coenzyme A. The protein product of this gene, dihydrolipoamide acetyltransferase, accepts acetyl groups formed by the oxidative decarboxylation of pyruvate and transfers them to coenzyme A. Dihydrolipoamide acetyltransferase is the antigen for antimitochondrial antibodies. These autoantibodies are present in nearly 95% of patients with the autoimmune liver disease primary biliary cirrhosis (PBC). In PBC, activated T lymphocytes attack and destroy epithelial cells in the bile duct where this protein is abnormally distributed and overexpressed. PBC enventually leads to cirrhosis and liver failure. Mutations in this gene are also a cause of pyruvate dehydrogenase E2 deficiency which causes primary lactic acidosis in infancy and early childhood
Entrez Summary: This gene encodes component E2 of the multi-enzyme pyruvate dehydrogenase complex (PDC). PDC resides in the inner mitochondrial membrane and catalyzes the conversion of pyruvate to acetyl coenzyme A. The protein product of this gene, dihydrolipoamide acetyltransferase, accepts acetyl groups formed by the oxidative decarboxylation of pyruvate and transfers them to coenzyme A. Dihydrolipoamide acetyltransferase is the antigen for antimitochondrial antibodies. These autoantibodies are present in nearly 95% of patients with the autoimmune liver disease primary biliary cirrhosis (PBC). In PBC, activated T lymphocytes attack and destroy epithelial cells in the bile duct where this protein is abnormally distributed and overexpressed. PBC enventually leads to cirrhosis and liver failure. Mutations in this gene are also a cause of pyruvate dehydrogenase E2 deficiency which causes primary lactic acidosis in infancy and early childhood
Product Categories/Family for anti-DLAT antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
69kDa
NCBI Official Full Name
dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial
NCBI Official Synonym Full Names
dihydrolipoamide S-acetyltransferase
NCBI Official Symbol
DLAT
NCBI Official Synonym Symbols
DLTA; PDCE2; PDC-E2
NCBI Protein Information
dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial
UniProt Protein Name
Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial
UniProt Gene Name
DLAT
UniProt Synonym Gene Names
DLTA; PBC; PDC-E2; PDCE2
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Product Notes
The DLAT dlat (Catalog #AAA111276) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The DLAT (Pyruvate Dehydrogenase E2) Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's DLAT can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), ICC (Immunocytochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the DLAT dlat for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DLAT, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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