WB (Western Blot)
(Western Blot Analysis using AAA14655: Lane 1: MW Marker; Lane 2: DnaK active recombinant protein, Lane 3: E. coli cell lysate.)
Mouse anti-E Coli DnaK Monoclonal Antibody
DnaK
Reactivity
E Coli
Applications
ELISA, Western Blot
Purity
Affinity PurifiedPurified by Protein G affinity chromatography.
Synonyms
DnaK, Antibody; DnaK; Anti -DnaK; anti-DnaK antibody
Host
Mouse
Reactivity
E Coli
Clonality
Monoclonal
Isotype
IgG1
Clone Number
4E66
Specificity
Recognizes E. coli DnaK.
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Purified by Protein G affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide, 50% glycerol.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-DnaK antibody
ELISA (EL/EIA), Western Blot (WB)
Other applications not tested.
Other applications not tested.
Application Notes
Western Blot: 1:10,000.
Detects a band at ~70kD.
Optimal dilutions to be determined by researcher.
Positive control: Dnak, Recombinant, E.coli
Detects a band at ~70kD.
Optimal dilutions to be determined by researcher.
Positive control: Dnak, Recombinant, E.coli
Immunogen
E. coli DnaK
Preparation and Storage
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
WB (Western Blot)
(Western Blot Analysis using AAA14655: Lane 1: MW Marker; Lane 2: DnaK active recombinant protein, Lane 3: E. coli cell lysate.)
WB (Western Blot)
(Western Blot Analysis using AAA14655: Lane 1: MW Marker; Lane 2: DnaK active recombinant protein, Lane 3: E. coli cell lysate.)
Related Product Information for anti-DnaK antibody
DnaK possesses an autophosphorylation activity and a weak 5'-nucleotidase activity, cleaving the 5'-phosphate groups from both ribose- and deoxyribose nucleotides. Genetic and biochemical studies have demonstrated protein-protein interactions between the DnaK protein and the lambdaO and lambdaP DNA replicating proteins. DnaK has also been shown to interact with DnaJ and GrpE heat shock proteins. In addition, DnaK has been demonstrated to preferentially bind to the unfolded form of bovine pancreatic trypsin inhibitor as opposed to the native protein. The role of DnaK in ATP-dependent protein-protein interactions has been extended to normal E. coli physiology. Like eukaryotic Hsp70 homologs, it is thought to participate in the assembly/disassembly of protein complexes, essentially as first proposed by Hightower and Pelham.
Product Categories/Family for anti-DnaK antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI Official Full Name
DnaK
Similar Products
Product Notes
The DnaK (Catalog #AAA14655) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The DnaK reacts with E Coli and may cross-react with other species as described in the data sheet. AAA Biotech's DnaK can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB) Other applications not tested. Western Blot: 1:10,000. Detects a band at ~70kD. Optimal dilutions to be determined by researcher. Positive control: Dnak, Recombinant, E.coli. Researchers should empirically determine the suitability of the DnaK for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DnaK, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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