WB (Western Blot)
(Western blot analysis: Lane 1: MW Marker, Lane 2: DnaK active recombinant protein, Lane 3: E Coli cell lysate.)
Mouse anti-E Coli DnaK Monoclonal Antibody | anti-Hsp70 antibody
DnaK (E Coli) monoclonal antibody (8E2/2)
Gene Names
dnaK; ECK0014; groPAB; groPC; groPF; grpC; grpF; JW0013; seg
Reactivity
E Coli
Applications
Western Blot
Purity
Protein G affinity purified.
Synonyms
DnaK, Antibody; DnaK (E Coli) monoclonal antibody (8E2/2); DnaK; anti-Hsp70 antibody
Host
Mouse
Reactivity
E Coli
Clonality
Monoclonal
Isotype
IgG1
Clone Number
8E2/2
Specificity
Recognizes bacteria DnaK.
Purity/Purification
Protein G affinity purified.
Form/Format
Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide.
Sequence Length
638
Applicable Applications for anti-Hsp70 antibody
WB (Western Blot)
Immunogen
E Coli DnaK.
Preparation and Storage
Ships on blue ice.
Long term storage: -20°C
Avoid freeze/thaw cycles.
Long term storage: -20°C
Avoid freeze/thaw cycles.
WB (Western Blot)
(Western blot analysis: Lane 1: MW Marker, Lane 2: DnaK active recombinant protein, Lane 3: E Coli cell lysate.)
WB (Western Blot)
(Western blot analysis: Lane 1: MW Marker, Lane 2: DnaK active recombinant protein, Lane 3: E Coli cell lysate.)
Related Product Information for anti-Hsp70 antibody
DnaK possesses an autophosphorylation activity and a weak 5-nucleotidase activity, cleaving the 5 phosphate groups from both ribose and deoxyribose nucleotides. The role of DnaK in ATP-dependent protein-protein interactions has been extended to normal E Coli physiology, where, like eukaryotic Hsp70 homologs, it is thought to participate in the assembly/disassembly of protein complexes.
Product Categories/Family for anti-Hsp70 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI Official Full Name
Chaperone protein DnaK
NCBI Official Symbol
dnaK
NCBI Official Synonym Symbols
ECK0014; groPAB; groPC; groPF; grpC; grpF; JW0013; seg
NCBI Protein Information
chaperone Hsp70, with co-chaperone DnaJ
UniProt Protein Name
Chaperone protein DnaK
UniProt Gene Name
dnaK
UniProt Synonym Gene Names
groP; grpF; seg
UniProt Entry Name
DNAK_ECOLI
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Product Notes
The Hsp70 dnak (Catalog #AAA76986) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The DnaK (E Coli) monoclonal antibody (8E2/2) reacts with E Coli and may cross-react with other species as described in the data sheet. AAA Biotech's DnaK can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot). Researchers should empirically determine the suitability of the Hsp70 dnak for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DnaK, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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