Double Stranded DNA (dsDNA) Recombinant Antibody | dsDNA recombinant antibody

Double Stranded DNA (dsDNA) (Nuclear Marker)

Cat. Num. AAA215503

• Reactivity: Human
• Applications: Immunohistochemistry, Immunofluorescence, Flow Cytometry, Functional Assay
• Purity: Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Synonyms: Double Stranded DNA (dsDNA), Recombinant Antibody; Double Stranded DNA (dsDNA) (Nuclear Marker); dsDNA recombinant antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG / Kappa
• Clone Number: DSD/4054R
• Specificity: This monoclonal antibody is part of a new panel of reagents, which recognizes subcellular organelles or compartments of human cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. This MAb recognizes the double stranded DNA in human cells. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in human cells. This MAb produces a homogeneous staining pattern in the nucleus of normal and malignant cells.Double Stranded deoxyribonucleic acid (ds DNA) is the genetic material of all cells and many viruses and is a polymer of nucleotides. The monomer consists of phosphorylated 2-deoxyribose N-glycosidically linked to one of four bases, adenine, cytosine, guanine or thymine. These are linked together by 3-phosphodiester bridges. In the Watson-Crick double-helix model, two complementary strands are wound in a right-handed helix and held together by hydrogen bonds between complementary base pairs.
• Purity/Purification: Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Form/Format: 200ug/ml of Ab purified from Bioreactor Concentrate. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide.

• Applicable Applications for dsDNA recombinant antibody: IHC (Immunohistochemistry), IF (Immunofluorescence), FCM/FACS (Flow Cytometry)
• Immunogen: Nuclei of Burkitt's cells
• Cellular Localization: Nuclear
• Positive Control: Raji, Jurkat or HeLa cells. Human tonsil or Colon.
• Preparation and Storage: Store at 2-8°C

IHC (Immunohiostchemistry)

(Formalin-fixed, paraffin-embedded human colon carcinoma stained with dsDNA Recombinant Rabbit Monoclonal Antibody (DSD/4054R).)

IHC (Immunohistochemistry)

(Formalin-fixed, paraffin-embedded human tonsil stained with dsDNA Recombinant Rabbit Monoclonal Antibody (DSD/4054R).)

References

Epstein, A.L. and Clevenger, C.V., Identification of nuclear antigens in human cells by immunofluorescence, immunoelectron microscopy, and immuno-biochemical methods using monoclonal antibodies. In Progress on nonhistone protein research, Vol. 1, Isaac Bekhor, ed., 1985, CRC Press, Boca Raton, FL, pp 117-137.