IF (Immunofluorescence)
(Immunofluorescence staining of 293T cells transfected with DYKDDDDK tag by AAA18845 at 1:300, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-mouse IgG(H+L). The image on the right is the 293T cells transfected without DYKDDDDK tag.)
Mouse anti-All DYKDDDDK Tag Monoclonal Antibody
Mouse Anti-DYKDDDDK Tag monoclonal Antibody
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
IF: 1:100-1:300
IP: 0.5ul-2ul
IF (Immunofluorescence)
(Immunofluorescence staining of 293T cells transfected with DYKDDDDK tag by AAA18845 at 1:300, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-mouse IgG(H+L). The image on the right is the 293T cells transfected without DYKDDDDK tag.)
IF (Immunofluorescence)
(Immunofluorescence staining of 293T cells transfected with DYKDDDDK tag by AAA18845 at 1:300, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-mouse IgG(H+L). The image on the right is the 293T cells transfected without DYKDDDDK tag.)
IP (Immunoprecipitation)
(Immunoprecipitating DYKDDDDK Tag in 293F transfected whole cell lysateLane 1: Mouse control IgG instead of AAA18845 in DYKDDDDK Tag in 293F transfected whole cell lysate. For western blotting, a HRPconjugated Protein G antibody was used as the secondary antibody (1/2000)Lane 2: Company A (1ul), Company B (1ul), Company C (1ul), Company D (1ul), AAA18845 (1ul) + DYKDDDDK Tag in 293F transfected whole cell lysate (500ug)Lane 3: DYKDDDDK Tag in 293F transfected whole cell lysate (20ug))
IP (Immunoprecipitation)
(Immunoprecipitating DYKDDDDK Tag in 293F transfected whole cell lysateLane 1: Mouse control IgG instead of AAA18845 in DYKDDDDK Tag in 293F transfected whole cell lysate. For western blotting, a HRPconjugated Protein G antibody was used as the secondary antibody (1/2000)Lane 2: Company A (1ul), Company B (1ul), Company C (1ul), Company D (1ul), AAA18845 (1ul) + DYKDDDDK Tag in 293F transfected whole cell lysate (500ug)Lane 3: DYKDDDDK Tag in 293F transfected whole cell lysate (20ug))
IP (Immunoprecipitation)
(Immunoprecipitating DYKDDDDK Tag in Transfected HEK-293 cell whole cell lysateLane 1: Mouse control IgG instead of AAA18845 in Transfected HEK-293 cell whole cell lysateLane 2: AAA18845 (1ul) + Transfected HEK-293 cell whole cell lysate (500ug)Lane 3: Transfected HEK-293 cell whole cell lysate (20?g)For western blotting, the blot was detected with AAA18845 at 1:5000, and a HRPconjugated Protein G antibody was used as the secondary antibody at 1:2000)
IP (Immunoprecipitation)
(Immunoprecipitating DYKDDDDK Tag in Transfected HEK-293 cell whole cell lysateLane 1: Mouse control IgG instead of AAA18845 in Transfected HEK-293 cell whole cell lysateLane 2: AAA18845 (1ul) + Transfected HEK-293 cell whole cell lysate (500ug)Lane 3: Transfected HEK-293 cell whole cell lysate (20?g)For western blotting, the blot was detected with AAA18845 at 1:5000, and a HRPconjugated Protein G antibody was used as the secondary antibody at 1:2000)
WB (Western Blot)
(Western BlotPositive WB detected in: 5 different recombinant proteins with DYKDDDDK TagAll lanes: Company A, Company B, Company C, Company D, AAA18845 antibody at 1:5000SecondaryGoat polyclonal to Mouse IgG at 1/50000 dilution)
WB (Western Blot)
(Western BlotPositive WB detected in: 5 different recombinant proteins with DYKDDDDK TagAll lanes: Company A, Company B, Company C, Company D, AAA18845 antibody at 1:5000SecondaryGoat polyclonal to Mouse IgG at 1/50000 dilution)
WB (Western Blot)
(Western BlotPositive WB detected in: DYKDDDDK Tag fusion protein at 50ng, 25ng, 12.5ng, 6.25ng, 3.125ng, 1.5625ng, 0.78ng, 0.39ngAll lanes: Company A, Company B, Company C, Company D, AAA18845 antibody at 1:1000SecondaryGoat polyclonal to Mouse IgG at 1/50000 dilutionPredicted band size: 35 kDaObserved band size: 35 kDa)
WB (Western Blot)
(Western BlotPositive WB detected in: DYKDDDDK Tag fusion protein at 50ng, 25ng, 12.5ng, 6.25ng, 3.125ng, 1.5625ng, 0.78ng, 0.39ngAll lanes: Company A, Company B, Company C, Company D, AAA18845 antibody at 1:1000SecondaryGoat polyclonal to Mouse IgG at 1/50000 dilutionPredicted band size: 35 kDaObserved band size: 35 kDa)
WB (Western Blot)
(Western BlotPositive WB detected in: DYKDDDDK Tag fusion protein at 10ngAll lanes: Company A, Company B, Company C, Company D, AAA18845 antibody at 1:50000, 1:100000, 1:200000, 1:400000, 1:800000, 1:1600000, 1:3200000, 1:6400000SecondaryGoat polyclonal to Mouse IgG at 1/50000 dilutionPredicted band size: 35 kDaObserved band size: 35 kDa)
WB (Western Blot)
(Western BlotPositive WB detected in: DYKDDDDK Tag fusion protein at 10ngAll lanes: Company A, Company B, Company C, Company D, AAA18845 antibody at 1:50000, 1:100000, 1:200000, 1:400000, 1:800000, 1:1600000, 1:3200000, 1:6400000SecondaryGoat polyclonal to Mouse IgG at 1/50000 dilutionPredicted band size: 35 kDaObserved band size: 35 kDa)
WB (Western Blot)
(Western BlotPositive WB detected in: DYKDDDDK Tag fusion protein 1, 2, 3, 4 at 50ngAll lanes: DYKDDDDK Tag antibody at 1:1000SecondaryGoat polyclonal to mouse IgG at 1/50000 dilutionPredicted band size: 59.6, 36, 35, 42 kDaObserved band size: 59.6, 36, 35, 42 kDa)
WB (Western Blot)
(Western BlotPositive WB detected in: DYKDDDDK Tag fusion protein 1, 2, 3, 4 at 50ngAll lanes: DYKDDDDK Tag antibody at 1:1000SecondaryGoat polyclonal to mouse IgG at 1/50000 dilutionPredicted band size: 59.6, 36, 35, 42 kDaObserved band size: 59.6, 36, 35, 42 kDa)
WB (Western Blot)
(Western BlotPositive WB detected in: DYKDDDDK Tag fusion protein at 50ng, 25ng, 12.5ng, 6.25ng, 3.625ng, 1.5625ng, 0.78ngAll lanes: DYKDDDDK Tag antibody at 1:1000SecondaryGoat polyclonal to mouse IgG at 1/50000 dilutionPredicted band size: 35 kDaObserved band size: 35 kDa)
WB (Western Blot)
(Western BlotPositive WB detected in: DYKDDDDK Tag fusion protein at 50ng, 25ng, 12.5ng, 6.25ng, 3.625ng, 1.5625ng, 0.78ngAll lanes: DYKDDDDK Tag antibody at 1:1000SecondaryGoat polyclonal to mouse IgG at 1/50000 dilutionPredicted band size: 35 kDaObserved band size: 35 kDa)
WB (Western Blot)
(Western BlotPositive WB detected in: 10ng DYKDDDDK Tag fusion protein DYKDDDDK Tag antibody at 1:100000, 1:200000, 1:400000, 1:800000, 1:1600000SecondaryGoat polyclonal to mouse IgG at 1/50000 dilutionPredicted band size: 35 kDaObserved band size: 35 kDa)
WB (Western Blot)
(Western BlotPositive WB detected in: 10ng DYKDDDDK Tag fusion protein DYKDDDDK Tag antibody at 1:100000, 1:200000, 1:400000, 1:800000, 1:1600000SecondaryGoat polyclonal to mouse IgG at 1/50000 dilutionPredicted band size: 35 kDaObserved band size: 35 kDa)
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Product Notes
The DYKDDDDK Tag (Catalog #AAA18845) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse Anti-DYKDDDDK Tag monoclonal Antibody reacts with All and may cross-react with other species as described in the data sheet. AAA Biotech's DYKDDDDK Tag can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunoprecipitation (IP), Immunofluorescence (IF). WB: 1:1,000-1:10,000 IF: 1:100-1:300 IP: 0.5ul-2ul. Researchers should empirically determine the suitability of the DYKDDDDK Tag for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DYKDDDDK Tag, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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