Mouse anti-All DYKDDDDK-Tag Monoclonal Antibody

DYKDDDDK-Tag (3B9) Mouse mAb

Cat. Num. AAA62295

• Reactivity: All
• Applications: ELISA, Immunoprecipitation, Immunofluorescence, Western Blot
• Synonyms: DYKDDDDK-Tag, Antibody; DYKDDDDK-Tag (3B9) Mouse mAb; anti-DYKDDDDK-Tag antibody

• Host: Mouse
• Reactivity: All
• Clonality: Monoclonal
• Isotype: Mouse IgG2b
• Specificity: DYKDDDDK Tag Antibody detects exogenously expressed DYKDDDDK proteins in cells.

• Applicable Applications for anti-DYKDDDDK-Tag antibody: ELISA, IP (Immunoprecipitation), IF (Immunofluorescence), WB (Western Blot)
• Source Note: This monoclonal antibody is produced by immunizing animals with a synthetic DYKDDDDK peptide (KLH-coupled).
• Preparation and Storage: Store at -20 degree C. Stable for one year from the date of shipment.

IF (Immunofluorescence)

(IF analysis of 293T cells transfected with a DYKDDDDK-tagged protein using DYKDDDDK-Tag (3B9) Mouse mAb at a 1:1000 dilution.)

WB (Western Blot)

(Lane 1: 293T cell lysate transfected with DYKDDDDK-tag protein.Lane 2: Mock transfected 293T cell lysate. The antibody dilution is 1:100.)

WB (Western Blot)

(Western blot analysis of over-erpressed DYKDDDDK-tagged protein in 293T cell lysate, using DYKDDD0K-tag (3B9) Mouse mAb. The antibody dilutions are 1:2000 (lane 1). 1:5000 (lane 2). 1:1000 (lane 3) and 1:20000 (lane 4). Each lane was loaded with 2 ug of cell lysate. Lane 5 is 2 ug of mock transfected celL lysate at a dilution of 1:5000.)

Related Product Information for anti-DYKDDDDK-Tag antibody

Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation and immunostaining techniques. Due to their small size, they are unlikely to affect the tagged protein's biochemical properties. The DYKDDDDK peptide has been used extensively as a general epitope tag in expression vectors. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion.

Product Categories/Family for anti-DYKDDDDK-Tag antibody

Tag

References

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