IF (Immunofluorescence)
( Immunofluoresence staining of fixed HeLa cells with anti-ED-B domain of fibronectin antibody G4 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of G4 at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom DAPI, merged channels and a negative control. The negative control was stained with unimmunized rabbit IgG followed by Alexa Fluor 488 secondary antibody.)
ED-B domain of fibronectin Recombinant Antibody | EDB(+) FN recombinant antibody
Anti-ED-B domain of fibronectin [G4]
Reactivity
Human
Applications
Immunohistochemistry, Western Blot, ELISA
Purity
PurifiedProtein A Affinity Purified
Synonyms
ED-B domain of fibronectin, Recombinant Antibody; Anti-ED-B domain of fibronectin [G4]; Extra domain-B containing fibronectin; EDB(+) FN recombinant antibody
Reactivity
Human
Clonality
Monoclonal
Isotype
Rabbit IgG, kappa
Clone Number
G4
Specificity
This antibody bind to human Extra-Domain containing fibronectin.
Purity/Purification
Purified
Protein A Affinity Purified
Protein A Affinity Purified
Form/Format
PBS only with 0.02% Proclin 300.
Concentration
1mg/mL (varies by lot)
Applicable Applications for EDB(+) FN recombinant antibody
IHC (Immunohistochemistry), WB (Western Blot), ELISA
Immunogen
2D-PAGE gel isolated human Extra-Domain containing fibronectin from lysate of human melanoma COLO-38 cells.
Chimeric Use Statement
This full-length, chimeric rabbit antibody was made using the variable domain sequences of the original Human scFv format, for improved compatibility with existing reagents, assays and techniques.
Publication Note
Describes generation, using phage display, of antibodies with subnanomolar affinity against markers of angiogenesis.
Preparation and Storage
Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
IF (Immunofluorescence)
( Immunofluoresence staining of fixed HeLa cells with anti-ED-B domain of fibronectin antibody G4 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of G4 at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom DAPI, merged channels and a negative control. The negative control was stained with unimmunized rabbit IgG followed by Alexa Fluor 488 secondary antibody.)
IF (Immunofluorescence)
( Immunofluoresence staining of fixed HeLa cells with anti-ED-B domain of fibronectin antibody G4 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of G4 at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom DAPI, merged channels and a negative control. The negative control was stained with unimmunized rabbit IgG followed by Alexa Fluor 488 secondary antibody.)
Related Product Information for EDB(+) FN recombinant antibody
Recombinant monoclonal antibody to ED-B domain of fibronectin. Manufactured using Recombinant Platform with variable regions (i.e. specificity) from the phage display antibodyG4.
Product Categories/Family for EDB(+) FN recombinant antibody
References
Pini et al. J Biol Chem. 1998 Aug 21;273(34):21769-76. PMID:9705314
Similar Products
Product Notes
The EDB(+) FN (Catalog #AAA71975) is a Recombinant Antibody and is intended for research purposes only. The product is available for immediate purchase. The Anti-ED-B domain of fibronectin [G4] reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ED-B domain of fibronectin can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the EDB(+) FN for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ED-B domain of fibronectin, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.