ICC (Immunocytochemistry)
(Immunocytochemistry staining of HeLa cells fixed with -20? Methanol and using anti-eEF2 mouse mAb (dilution 1:200).)
Mouse anti-Human eEF2 Monoclonal Antibody | anti-eEF2 antibody
eEF2 Mouse mAb
Gene Names
EEF2; EF2; EF-2; EEF-2
Reactivity
Human
Applications
Immunocytochemistry, Western Blot
Purity
The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.
Synonyms
eEF2, Antibody; eEF2 Mouse mAb; EEF2; Eef2; EF-2; EF2; EF2_HUMAN; Elongation factor 2; Eukaryotic translation elongation factor 2; Polypeptidyl tRNA translocase; SCA26.; anti-eEF2 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Specificity
This antibody detects endogenous levels of eEF2 and does not cross-react with related proteins.
Purity/Purification
The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.
Form/Format
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-eEF2 antibody
ICC (Immunocytochemistry), WB (Western Blot)
Immunogen
Purified recombinant human eEF2 protein fragments expressed in E coli.
Modification
Unmodified/Total
Preparation and Storage
Store at -20 degree C/1 year
ICC (Immunocytochemistry)
(Immunocytochemistry staining of HeLa cells fixed with -20? Methanol and using anti-eEF2 mouse mAb (dilution 1:200).)
ICC (Immunocytochemistry)
(Immunocytochemistry staining of HeLa cells fixed with -20? Methanol and using anti-eEF2 mouse mAb (dilution 1:200).)
WB (Western Blot)
(Western blot detection of eEF2 in HL-60.Jurkat.SHSY-5Y.U20S and Hela cell lysates using eEF2 mouse mAb (1:5000 diluted).Predicted band size:95KDa.Observed band size: 95KDa.Exposure time:15s.)
WB (Western Blot)
(Western blot detection of eEF2 in HL-60.Jurkat.SHSY-5Y.U20S and Hela cell lysates using eEF2 mouse mAb (1:5000 diluted).Predicted band size:95KDa.Observed band size: 95KDa.Exposure time:15s.)
Related Product Information for anti-eEF2 antibody
This gene encodes a member of the GTP-binding translation elongation factor family. This protein is an essential factor for protein synthesis. It promotes the GTP-dependent translocation of the nascent protein chain from the A-site to the P-site of the ribosome. This protein is completely inactivated by EF-2 kinase phosporylation. [provided by RefSeq, Jul 2008]
Product Categories/Family for anti-eEF2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
95,338 Da
NCBI Official Full Name
elongation factor 2
NCBI Official Synonym Full Names
eukaryotic translation elongation factor 2
NCBI Official Symbol
EEF2
NCBI Official Synonym Symbols
EF2; EF-2; EEF-2
NCBI Protein Information
elongation factor 2; polypeptidyl-tRNA translocase
UniProt Protein Name
Elongation factor 2
UniProt Gene Name
EEF2
UniProt Synonym Gene Names
EF2; EF-2
UniProt Entry Name
EF2_HUMAN
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Product Notes
The eEF2 eef2 (Catalog #AAA320339) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The eEF2 Mouse mAb reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's eEF2 can be used in a range of immunoassay formats including, but not limited to, ICC (Immunocytochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the eEF2 eef2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "eEF2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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