Mouse EFHD1 Monoclonal Antibody | anti-EFHD1 antibody

EFHD1 (5C10) Mouse mAb

Cat. Num. AAA23993

• Gene Names: EFHD1; SWS2; MST133; PP3051; MSTP133
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence
• Purity: Affinity purified
• Synonyms: EFHD1, Antibody; EFHD1 (5C10) Mouse mAb; EF-hand domain-containing protein D1; Mitocalcin; MGC103094; Swiprosin 2; anti-EFHD1 antibody

• Host: Mouse
• Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 5C10-4F1-10A5
• Specificity: The antibody detects endogenous EFHD1 proteins.
• Purity/Purification: Affinity purified
• Form/Format: PBS, pH7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.

• Applicable Applications for anti-EFHD1 antibody: Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunocytochemistry (ICC), Immunofluorescence (IF)
• Application Notes: WB: 1:2000; FC/FACS: 1:100-200; IHC: 1:50-300
• Immunogen: Synthetic Peptide of EFHD1
• Conjugation: Unconjugated
• Modification: Unmodified
• Cell Position: Membrane, Mitochondrion, Mitochondrion inner membrane
• Preparation and Storage: Store at 4 degree C for short term. Store at -20 degree C for long term. Avoid freeze/thaw cycle.

IF (Immunofluorescence)

(IF analysis of Hela with antibody (Left) and DAPI (Right) diluted at 1:100.)

WB (Western Blot)

(Western blot analysis of 1) Mouse spleen tissue, 2) Rat spleen tissue, diluted at 1:3000.)

IF (Immunofluorescence)

(Immunofluorescence analysis of Mouse-lung tissue. 1,EFHD1 Monoclonal Antibody(3G2)(red) was diluted at 1:200(4 degree C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded Mouse-testis tissue. 1,EFHD1 Monoclonal Antibody(3G2) was diluted at 1:200(4 degree C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded Rat-heart tissue. 1,EFHD1 Monoclonal Antibody(3G2) was diluted at 1:200(4 degree C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded Human-uterus tissue. 1,EFHD1 Monoclonal Antibody(3G2) was diluted at 1:200(4 degree C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.)

Related Product Information for anti-EFHD1 antibody

Acts as a calcium sensor for mitochondrial flash (mitoflash) activation, an event characterized by stochastic bursts of superoxide production (PubMed:26975899). May play a role in neuronal differentiation.

Product Categories/Family for anti-EFHD1 antibody

Primary antibody

NCBI and Uniprot Product Information

• NCBI GI #: 20149496
• NCBI GeneID: 80303
• NCBI Accession #: NP_079478
• NCBI GenBank Nucleotide #: NM_025202.3
• UniProt Accession #: Q9BUP0; Q9BTF8; Q9H8I2; Q9HBQ0; B2RD83; E9PFH3
• Molecular Weight: 16,145 Da
• NCBI Official Full Name: EF-hand domain-containing protein D1 isoform 1
• NCBI Official Synonym Full Names: EF-hand domain family, member D1
• NCBI Official Symbol: EFHD1
• NCBI Official Synonym Symbols: SWS2; MST133; PP3051; MSTP133
• NCBI Protein Information: EF-hand domain-containing protein D1; swiprosin-2; EF-hand domain-containing protein 1
• UniProt Protein Name: EF-hand domain-containing protein D1
• UniProt Gene Name: EFHD1
• UniProt Synonym Gene Names: SWS2
• UniProt Entry Name: EFHD1_HUMAN