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IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded Lung carcinoma using EGF Receptor (3F12) mouse mAb (1/800 dilution) at the Roche Benchmark XT system.)
Mouse anti-Human EGF Receptor Monoclonal Antibody | anti-EGFR antibody
EGF Receptor Antibody
Gene Names
EGFR; ERBB; HER1; mENA; ERBB1; PIG61; NISBD2
Reactivity
Human
Applications
Immunohistochemistry, Immunoprecipitation, Immunocytochemistry, Western Blot
Synonyms
EGF Receptor, Antibody; EGF Receptor Antibody; Purified Mouse Monoclonal Antibody (Mab); Avian erythroblastic leukemia viral (v erb b) oncogene homolog; Cell growth inhibiting protein 40; Cell proliferation inducing protein 61; EGF R; EGFR; EGFR_HUMAN; Epidermal growth factor receptor (avian erythroblastic leukemia viral (v erb b) oncogene homolog); Epidermal growth factor receptor (erythroblastic leukemia viral (v erb b) oncogene homolog avian); Epidermal growth factor receptor; erbb 1; Erbb; Erbb1; ERBB1; Errp; HER1; mENA; Oncogene ERBB; PIG61; Proto-oncogene c-ErbB-1; Receptor tyrosine protein kinase ErbB 1; Receptor tyrosine-protein kinase ErbB-1; Urogastrone; wa2; Wa5; anti-EGFR antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Form/Format
Purified mouse monoclonal antibody in PBS(pH 7.4) containing with 0.02% sodium azide and 50% glycerol.
Sequence Length
1210
Applicable Applications for anti-EGFR antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation), ICC (Immunocytochemistry), WB (Western Blot)
Antigen Source
Human
Antigen Type
Recombinant Protein
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded Lung carcinoma using EGF Receptor (3F12) mouse mAb (1/800 dilution) at the Roche Benchmark XT system.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded Lung carcinoma using EGF Receptor (3F12) mouse mAb (1/800 dilution) at the Roche Benchmark XT system.)
IP (Immunoprecipitation)
(Immunoprecipitation analysis of Hela cell lysates using EGFR mouse mAb.)
IP (Immunoprecipitation)
(Immunoprecipitation analysis of Hela cell lysates using EGFR mouse mAb.)
ICC (Immunocytochemistry)
(Immunocytochemistry staining of HeLa cells using EGFR mouse mAb (dilution 1:200).)
ICC (Immunocytochemistry)
(Immunocytochemistry staining of HeLa cells using EGFR mouse mAb (dilution 1:200).)
WB (Western Blot)
(Western blot detection of EGFR in HCC827,A549 and MDA-MB-468 cell lysates using EGFR mouse mAb(dilution 1:1000).Predicted band size:134 Kda.Observed band size:175KDa.)
WB (Western Blot)
(Western blot detection of EGFR in HCC827,A549 and MDA-MB-468 cell lysates using EGFR mouse mAb(dilution 1:1000).Predicted band size:134 Kda.Observed band size:175KDa.)
Related Product Information for anti-EGFR antibody
Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS- RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. May also activate the NF-kappa-B signaling cascade. Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling. Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin.
Product Categories/Family for anti-EGFR antibody
References
Ullrich A.,et al.Nature 309:418-425(1984). Ilekis J.V.,et al.Mol. Reprod. Dev. 41:149-156(1995). Reiter J.L.,et al.Nucleic Acids Res. 24:4050-4056(1996). Ilekis J.V.,et al.Gynecol. Oncol. 65:36-41(1997). Reiter J.L.,et al.Genomics 71:1-20(2001).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
134277
NCBI Official Full Name
epidermal growth factor receptor isoform a
NCBI Official Synonym Full Names
epidermal growth factor receptor
NCBI Official Symbol
EGFR
NCBI Official Synonym Symbols
ERBB; HER1; mENA; ERBB1; PIG61; NISBD2
NCBI Protein Information
epidermal growth factor receptor
UniProt Protein Name
Epidermal growth factor receptor
UniProt Gene Name
EGFR
UniProt Synonym Gene Names
ERBB; ERBB1; HER1
UniProt Entry Name
EGFR_HUMAN
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Product Notes
The EGFR egfr (Catalog #AAA290515) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The EGF Receptor Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's EGF Receptor can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation), ICC (Immunocytochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the EGFR egfr for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EGF Receptor, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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