Mouse EGFR (Epidermal Growth Factor Receptor) Monoclonal Antibody | anti-EGFR antibody

EGFR (Epidermal Growth Factor Receptor)

Cat. Num. AAA214937

• Gene Names: EGFR; ERBB; HER1; mENA; ERBB1; PIG61; NISBD2
• Reactivity: Human; Does not react with Mouse.
• Applications: Immunofluorescence, Flow Cytometry, Functional Assay
• Purity: Purified Ab conjugated to CF488
• Synonyms: EGFR (Epidermal Growth Factor Receptor), Antibody; EGFR (Epidermal Growth Factor Receptor); Erbb1; ERBB1; Errp; HER1; mENA; PIG61; Proto-oncogene c-ErbB-1; Receptor Tyrosine Protein Kinase; ErbB1; Urogastrone; wa2; Wa5; anti-EGFR antibody

• Host: Mouse
• Reactivity: Human; Does not react with Mouse.
• Clonality: Monoclonal
• Isotype: IgG2a, kappa
• Clone Number: GFR450
• Specificity: This MAb recognizes a protein of 170kDa, identified as EGFR. EGFR is type I receptor tyrosine kinase with sequence homology to erbB-1, -2, -3 -4 or HER-1, -2, -3 -4. It binds to Epidermal Growth Factor (EGF), Transforming Growth Factor-a (TGF-a), Heparin-binding EGF (HB-EGF), amphiregulin, betacellulin and epiregulin. EGFR is overexpressed in tumors of breast, brain, bladder, lung, gastric, head neck, esophagus, cervix, vulva, ovary, and endometrium. It is predominantly present in squamous cell carcinomas.
• Purity/Purification: Purified Ab conjugated to CF488
• Form/Format: Antibody Purified from Bioreactor Concentrate by Protein A/G and conjugated to various reporter molecules. Prepared in 10mM PBS with 0.05% BSA and 0.05% azide. Contact us if you require this Ab in a different format.
• Sequence Length: 1210

• Applicable Applications for anti-EGFR antibody: IF (Immunofluorescence), FCM/FACS (Flow Cytometry)
• Positive Control: A431 cells, Breast or Bladder cancer.
• Immunogen: Recombinant extracellular domain of human EGFR protein
• Cellular Localization: Cell Surface
• Hu-chromosome Location: 7p11.2
• Preparation and Storage: Antibody with azide: store at 4 to 8 degree C.; Antibody is stable for 24 months. Non-hazardous. No MSDS required.

IF (Immunofluorescence)

(Confocal Immunofluorescent analysis of A431 cells using CF488-labeled Isotype Control MAb (IgG2a) (Green). DAPI was used to stain the cell nuclei (blue). (Negative Control))

IF (Immunofluorescence)

(Confocal Immunofluorescent analysis of A431 cells using CF488-labeled EGFR Monoclonal Antibody (GFR450) (Green). DAPI was used to stain the cell nuclei (blue).)

FCM/FACS (Flow Cytometry)

(Flow Cytometry of EGFR on Mouse NIH/3T3 cells. Black: cells alone; Grey: Isotype Control; Green: CF488-labeled EGFR Monoclonal Antibody (GFR450).)

FCM/FACS (Flow Cytometry)

(Flow Cytometry of human EGFR on A431 cells. Black: cells alone; Grey: Isotype Control; Green: CF488-labeled EGFR Monoclonal Antibody (GFR450).)

References

Tungekar MF et. al. Journal of Clinical Pathology. 51: 583-587 (1998)

NCBI and Uniprot Product Information

• NCBI GI #: 29725609
• NCBI GeneID: 1956
• NCBI Accession #: NP_005219.2
• NCBI GenBank Nucleotide #: NP_005219.2
• UniProt Accession #: P00533
• Molecular Weight: ~170kDa (wild type) and ~145kDa (vIII variant)
• NCBI Official Full Name: epidermal growth factor receptor isoform a
• NCBI Official Synonym Full Names: epidermal growth factor receptor
• NCBI Official Symbol: EGFR
• NCBI Official Synonym Symbols: ERBB; HER1; mENA; ERBB1; PIG61; NISBD2
• NCBI Protein Information: epidermal growth factor receptor
• UniProt Protein Name: Epidermal growth factor receptor
• UniProt Gene Name: EGFR
• UniProt Synonym Gene Names: ERBB; ERBB1; HER1
• UniProt Entry Name: EGFR_HUMAN