Rat anti-Mouse Emilin-1 Monoclonal Antibody | anti-Emilin1 antibody

Rat Anti-Mouse Emilin-1

Cat. Num. AAA14943

• Gene Names: Emilin1; gp115; AW229038; BB105748; EMILIN-1; 5830419M17Rik
• Reactivity: Mouse
• Applications: Western Blot, Immunohistochemistry
• Purity: Protein G purified
• Synonyms: Emilin-1, Antibody; Rat Anti-Mouse Emilin-1; Elastin micofibril interface-located protein 1; anti-Emilin1 antibody

• Host: Rat
• Reactivity: Mouse
• Clonality: Monoclonal
• Clone Number: IgG2a/kappa, C11A8
• Purity/Purification: Protein G purified
• Form/Format: Lyophilized; PBS
• Sequence Length: 1017

• Applicable Applications for anti-Emilin1 antibody: Western Blot (WB), Immunohistochemistry (IHC) - Formalin
• Application Notes: Western Blot: Use at 2-10 ug/ml IF/IHC: Frozen sections; ; Optimal dilutions should determined by each laboratory for each application
• Immunogen: recombinant mouse Emilin-1
• Preparation: Monoclonals were produced with the help of Wistar rat using recombinant murine Emilin-2 as immunizing antigen
• Reconstitution: Centrifuge vial prior to opening. Reconstitute in sterile water to concentration of 0.1-1.0 mg/ml.
• Preparation and Storage: The lyophilized antibody is stable at room temperature for up to 1 month. The reconstituted antibody is stable for at least two weeks at 2-8 degree C. Frozen aliquots are stable for at least 6 months when stored at -20 degree C. AVOID REPEATED FREEZE AND THAW CYCLES!

IF (Immunofluorescence)

(MILIN1 is expressed in association with lymphatic vessels. Cryostat sections of normal mouse tissues stained with anti-EMILIN1 (green) antibodies. In all mouse tissues and organs examined, EMILIN1 was uniformly distributed in the stroma. In the skin, EMILIN1 staining colocalizes with LYVE-1-positive lymphatic vessels surrounding hair follicles. In the small intestine, EMILIN1 colocalizes with LYVE-1-positive lacteals and submucosal lymphatic vessels. At higher magnification, in the lung and lymph nodes, it is more evident that EMILIN1 is distributed at the abluminal surfaces of LECs. In the lymph node, EMILIN1-positive fibers connecting LECs to the surrounding ECM are evident.)

IF (Immunofluorescence)

(Positive staining of EMILIN1 (green) in mouse lymphatic endothelial cells (LAEC). Nuclei are stained in blue. Scale bar 38 um)

IF (Immunofluorescence)

(Positive staining of EMILIN1 (green) in mouse fibroblasts (NIH 3T3). Actin cytoskeleton is stained in red, nuclei in blue. Scale bar 28 um)

IF (Immunofluorescence)

(Positive staining for EMILIN1 (red) in mouse skin. Blue, nuclei;Scale bar: 37.00 um)

IF (Immunofluorescence)

(Positive staining for EMILIN1 green) in mouse lymph node. In red, lymphatic vessels positive for Lyve-1. Yellow represents the closeassociation of EMILIN1 with lymphatic vessel structures. Scale bar: 37.00 ?m)

IF (Immunofluorescence)

(Positive staining for EMILIN1 (green) in mouse lung tissue is both detected in lung parenchymal fibers and associated with lymphatic vessel structures. Lyve-1 (red) is used as lymphatic vessel marker. Blue, nuclei.Scale bar: 37.00 um)

WB (Western Blot)

(Western blot on recombinant protein obtained from supernatant of cells expressing human EMILIN1 (hE1), mouse EMILIN1 (mE1), mouse EMILIN2 (mE2).)

Related Product Information for anti-Emilin1 antibody

Emilin-1 is an extracellular matrix glycoprotein localized at sites where elastin and microfibrils are in proximity. It may be responsible for anchoring smooth muscle cells to elastic fibers. It has cell adhesive capacity. Emilin-1 may have a role in the regulation of blood vessel assembly since it inhibits TGFB signaling by binding specifically to the pro-TGFB precursor and preventing its maturation by furin convertases in the extracellular space. TGFB proteins are the main regulators of blood vessel development and maintenance.

References

1. Colombatti A et al, J Cell Biol 100:18, 1985. 3. Doliana R et al, J Biol Chem 274:16773, 1999. 4. Zanetti M et al, Mol Cell Biol 24:638, 2004 5. Zacchigna L et al, Cell 124:929, 2006 6. Danussi C et al, Mol Cell Biol 28:4026, 2008. 7. Danussi C et al, J Cell Biol, in press

NCBI and Uniprot Product Information

• NCBI GI #: 19527130
• NCBI GeneID: 100952
• NCBI Accession #: NP_598679.1
• NCBI GenBank Nucleotide #: NM_133918.2
• UniProt Accession #: Q99K41
• NCBI Official Full Name: EMILIN-1
• NCBI Official Synonym Full Names: elastin microfibril interfacer 1
• NCBI Official Symbol: Emilin1
• NCBI Official Synonym Symbols: gp115; AW229038; BB105748; EMILIN-1; 5830419M17Rik
• NCBI Protein Information: EMILIN-1; elastin microfibril interface located protein 1; elastin microfibril interface-located protein 1
• UniProt Protein Name: EMILIN-1
• UniProt Gene Name: Emilin1
• UniProt Synonym Gene Names: Elastin microfibril interfacer 1
• UniProt Entry Name: EMIL1_MOUSE