WB (Western Blot)
(ENO1 Antibody western blot analysis in MCF-7,A431,Hela,HepG2,mouse NIH/3T3 cell line and mouse stomach,liver tissue lysates (35ug/lane).This demonstrates the ENO1 antibody detected the ENO1 protein (arrow).)
Mouse anti-Human, mouse ENO1 Monoclonal Antibody | anti-ENO1 antibody
ENO1 Antibody
Gene Names
ENO1; NNE; PPH; MPB1; ENO1L1
Reactivity
Human, mouse
Applications
Western Blot, Immunohistochemistry, ELISA
Synonyms
ENO1, Antibody; ENO1 Antibody; Alpha-enolase; 2-phospho-D-glycerate hydro-lyase; C-myc promoter-binding protein; Enolase 1; MBP-1; MPB-1; Non-neural enolase; NNE; Phosphopyruvate hydratase; Plasminogen-binding protein; ENO1; ENO1L1; MBPB1; MPB1; anti-ENO1 antibody
Host
Mouse
Reactivity
Human, mouse
Clonality
Monoclonal
Isotype
IgG (k)
Clone Number
786CT6.6.4
Specificity
Purified His-tagged ENO1 protein was used to produced this monoclonal antibody.
Form/Format
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
Sequence Length
341
Applicable Applications for anti-ENO1 antibody
WB (Western Blot), IHC (Immunohistochemistry), ELISA
Function
Multifunctional enzyme that, as well as its role in glycolysis, plays a part in various processes such as growth control, hypoxia tolerance and allergic responses. May also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons. Stimulates immunoglobulin production.
Cellular Location
Cytoplasm. Cell membrane. Cytoplasm, myofibril, sarcomere, M line. Note=Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form. ENO1 is localized to the M line.
Tissue Location
The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons.
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
WB (Western Blot)
(ENO1 Antibody western blot analysis in MCF-7,A431,Hela,HepG2,mouse NIH/3T3 cell line and mouse stomach,liver tissue lysates (35ug/lane).This demonstrates the ENO1 antibody detected the ENO1 protein (arrow).)
WB (Western Blot)
(ENO1 Antibody western blot analysis in MCF-7,A431,Hela,HepG2,mouse NIH/3T3 cell line and mouse stomach,liver tissue lysates (35ug/lane).This demonstrates the ENO1 antibody detected the ENO1 protein (arrow).)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded H. kideny section using ENO1 Antibody. AAA283454 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded H. kideny section using ENO1 Antibody. AAA283454 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.)
Related Product Information for anti-ENO1 antibody
Multifunctional enzyme that, as well as its role in glycolysis, plays a part in various processes such as growth control, hypoxia tolerance and allergic responses. May also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons. Stimulates immunoglobulin production.
MBP1 binds to the myc promoter and acts as a transcriptional repressor. May be a tumor suppressor.
MBP1 binds to the myc promoter and acts as a transcriptional repressor. May be a tumor suppressor.
Product Categories/Family for anti-ENO1 antibody
References
Giallongo A., et al. Proc. Natl. Acad. Sci. U.S.A. 83:6741-6745(1986).
Giallongo A., et al. Eur. J. Biochem. 190:567-573(1990).
Ray R., et al. Mol. Cell. Biol. 11:2154-2161(1991).
Walter M., et al. J. Autoimmun. 8:931-945(1995).
Kalnine N., et al. Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.
Giallongo A., et al. Eur. J. Biochem. 190:567-573(1990).
Ray R., et al. Mol. Cell. Biol. 11:2154-2161(1991).
Walter M., et al. J. Autoimmun. 8:931-945(1995).
Kalnine N., et al. Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
47169
NCBI Official Full Name
c-myc promoter-binding protein-1 isoform MBP-1
NCBI Official Synonym Full Names
enolase 1, (alpha)
NCBI Official Symbol
ENO1
NCBI Official Synonym Symbols
NNE; PPH; MPB1; ENO1L1
NCBI Protein Information
alpha-enolase; c-myc promoter-binding protein-1
UniProt Protein Name
Alpha-enolase
UniProt Gene Name
ENO1
UniProt Synonym Gene Names
ENO1L1; MBPB1; MPB1; NNE
UniProt Entry Name
ENOA_HUMAN
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Product Notes
The ENO1 eno1 (Catalog #AAA283454) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The ENO1 Antibody reacts with Human, mouse and may cross-react with other species as described in the data sheet. AAA Biotech's ENO1 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IHC (Immunohistochemistry), ELISA. Researchers should empirically determine the suitability of the ENO1 eno1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ENO1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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