Mouse ENO1 Monoclonal Antibody | anti-ENO1 antibody

ENO1 Antibody

Cat. Num. AAA60790

• Gene Names: ENO1; NNE; PPH; MPB1; ENO1L1
• Reactivity: Human, Mouse, Rat, Other
• Applications: Immunocytochemistry, Western Blot, Immunofluorescence
• Purity: Affinity purified
• Synonyms: ENO1, Antibody; ENO1 Antibody; ENO1; anti-ENO1 antibody

• Host: Mouse
• Reactivity: Human, Mouse, Rat, Other
• Clonality: Monoclonal
• Isotype: IgG1
• Purity/Purification: Affinity purified
• Form/Format: Liquid; PBS, pH 7.4 with 0.02% sodium azide.
• Sequence Length: 184

• Applicable Applications for anti-ENO1 antibody: ICC (Immunocytochemistry), WB (Western Blot), IF (Immunofluorescence)
• Immunogen: Enolases are enzymes which catalyze the conversion of 2-phosphoglycerate to phosphoenolpyruvate in the glycolytic pathway, and also the reverse reaction in gluconeogenesis. The Enzyme Commission classification number for this class of enzyme is EC4.2.1.11, and they can also be refered to as 2-phospho-D-glycerate hydrolases. There are three enolase proteins each coded for by a distinct gene. They are known as enolase 1, 2 and 3 or alternately as α, beta and gamma-enolase. Confusingly, although enolase 1 is alphaenolase, enolase 2 corresponds to gamma-enolase while enolase 3 is also known as beta-enolase. All three enzymes exist as dimers in vivo and the three molecules are very similar in primary amino acids sequence, as can be seen from a sequence alignment downloadable from here. This similarity allows not only homodimers but also most possible heterodimers to form. The expression pattern of the three enzymes differs in a tissue specific manner, so that antibodies to them are useful cell type specific markers, especially as all three molecules are very abundant. Enolase 1 or α is also known as non-neuronal enolase (NNE) and is expressed in most kinds of tissue, but is absent from neurons. Abnormal expression of NNE is associated with tumor progression in some breast and head and neck cancer (1, 2). Enolase 2 or gamma is also known as neuron specific enolase (NSE), and we also market antibodies directed against this protein, specifically RPCA-NSE. A switch from NNE to NSE occurs in the development of neurons (3). Enolase 3 or beta is expressed primarily in muscle cells. Monoclonal antibody MCA-253 was raised against the N-terminal 12 amino acids of bovine enolase 1 which was synthesized on a 8-amine lysine core using the multiple antigen presentation method (5). This produces a dendrimer presenting 8 peptides to the immune system. The characterization of the antibody has been published in peer reviewed form and in this publication is there referred to as Enol2-53 (4). The antibody was tested for binding to expressed bovine enolase 1, 2 and 3 and shown to be specific for only enolase 1. The HGNC name for this protein is ENO1.
• Preparation and Storage: Store at -20°C.

Application Data

WB (Western Blot)

References

1. Wu W, Tang X, Hu W, Lotan R, Hong WK, Mao L. Identification and validation of metastasis-associated proteins in head and neck cancer cell lines by two-dimensional electrophoresis and mass spectrometry. Clin Exp Metastasis.9(4):319-26 (2002). Clin Exp Metastasis. 19:319-26 (2002).2. Tu SH, Chang CC, Chen CS, Tam KW, Wang, YJ, Lee CH, Lin HW, Cheng TC, Huang CS, Chu JS, Shih NY, Chen LC, Leu SJ, Ho YS, Wu CH. Increased expression of enolase alpha in human breast cancer confers tamifer resistant in human breast cancer cells. Breast Cancer Res Treat. 121:539-53 (2010).3. Marangos PJ, Schmechel DE, Parma AM, Goodwin FK. Developmental profile of neuron-specific (NSE) and non-neuronal (NNE) enolase. Brain Res. 190:185-93 (1980).4. Smith WC, Bolch S, Dugger DR, Li,J, Esquenazi I, Arendt A, Benzenhafer D, McDowell JH. Interaction of arrestin with enolase 1 in photoreceptor. Invest Ophthalmol Vis Sci. 52:1832-40 (2011).5. Tam JP, Zavala F.J. Multiple antigen peptide. A novel approach to increase detection sensitivity of synthetic peptides in solid-phase immunoassays. J. Immunol Methods. 124:53-61 (1989).

NCBI and Uniprot Product Information

• NCBI GI #: 39644850
• NCBI GeneID: 2023
• UniProt Accession #: P06733; P22712; Q16704; Q4TUS4; Q53FT9; Q53HR3; Q658M5; Q6GMP2; Q71V37; Q7Z3V6; Q8WU71; B2RD59
• NCBI Official Full Name: ENO1 protein, partial
• NCBI Official Synonym Full Names: enolase 1, (alpha)
• NCBI Official Symbol: ENO1
• NCBI Official Synonym Symbols: NNE; PPH; MPB1; ENO1L1
• NCBI Protein Information: alpha-enolase; c-myc promoter-binding protein-1; enolase-alpha; tau-crystallin; non-neural enolase; alpha enolase like 1; phosphopyruvate hydratase; plasminogen-binding protein; MYC promoter-binding protein 1; 2-phospho-D-glycerate hydro-lyase
• UniProt Protein Name: Alpha-enolase
• UniProt Gene Name: ENO1
• UniProt Synonym Gene Names: ENO1L1; MBPB1; MPB1; NNE
• UniProt Entry Name: ENOA_HUMAN