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ICC (Immunocytochemistry)
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Mouse anti-Human, Mouse EP-CAM Monoclonal Antibody | anti-EP-CAM antibody

EP-CAM Antibody

Gene Names
EPCAM; ESA; KSA; M4S1; MK-1; DIAR5; EGP-2; EGP40; KS1/4; MIC18; TROP1; EGP314; HNPCC8; TACSTD1
Reactivity
Human, Mouse
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, Flow Cytometry, Functional Assay
Purity
ProL affinity purified
Synonyms
EP-CAM, Antibody; EP-CAM Antibody; 17 1A antibody; 323/A3 antibody; Adenocarcinoma associated antigen antibody; Adenocarcinoma-associated antigen antibody; Antigen identified by monoclonal antibody; AUA1 antibody; CD326 antibody; CD326 antigen antibody; Cell surface glycoprotein Trop 1 antibody; Cell surface glycoprotein Trop 2 antibody; Cell surface glycoprotein Trop-1 antibody; CO 17A antibody; CO17 1A antibody; CO17A antibody; DIAR5 antibody; EGP 2 antibody; EGP antibody; EGP2 antibody; EGP314 antibody; EGP40 antibody; Ep CAM antibody; Ep-CAM antibody; EPCAM antibody; EPCAM_HUMAN antibody; EpCAM1 antibody; Epithelial cell adhesion molecule antibody; Epithelial Cell Adhesion Molecule Intracellular Domain (EpCAM-ICD) antibody; Epithelial cell surface antigen antibody; Epithelial cellular adhesion molecule antibody; Epithelial glycoprotein 1 antibody; Epithelial glycoprotein 314 antibody; Epithelial glycoprotein antibody; ESA antibody; GA733 1 antibody; GA733 2 antibody; GA733-2 antibody; gastrointestinal tumor-associated antigen 2; 35-KD glycoprotein antibody; gp4 antibody; hEGP 2 antibody; hEGP314 antibody; HNPCC8 antibody; Human epithelial glycoprotein 2 antibody; KS 1/4 antigen antibody; KS1/4 antibody; KSA antibody; Ly74 antibody; Lymphocyte antigen 74 antibody; M1S 1 antibody; M1S2 antibody; M4S1 antibody; Major gastrointestinal tumor associated protein GA733 2 antibody; Major gastrointestinal tumor-associated protein GA733-2 antibody; mEGP314 antibody; Membrane component chromosome 4 surface marker (35kD glycoprotein) antibody; Membrane component; chromosome 4; surface marker 1 antibody; surface marker antibody; MIC18 antibody; MK 1 antibody; Protein 289A antibody; TACD1 antibody; TACSTD1 antibody; TROP1 antibody; Tumor associated calcium signal transducer 1 antibody; Tumor associated calcium signal transducer 2 precursor antibody; Tumor-associated calcium signal transducer 1 antibody; anti-EP-CAM antibody
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For Research Use Only!
Host
Mouse
Reactivity
Human, Mouse
Clonality
Monoclonal
Clone Number
3-E1-E2
Purity/Purification
ProL affinity purified
Form/Format
1*TBS (pH7.4), 1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Applicable Applications for anti-EP-CAM antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes
WB: 1:1000-1:2000
IHC: 1:200-1:500
ICC: 1:100-1:200
FC/FACS: 1:100-1:200
Immunogen
Recombinant protein
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C.

ICC (Immunocytochemistry)

(ICC staining EpCAM in SW1990 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry)

(ICC staining EpCAM in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry)

(ICC staining EpCAM in D3 cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using EpCAM antibody. Counter stained with)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using EpCAM antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded human kidney tissue using EpCAM antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using EpCAM antibody. Counter stained with hematoxylin.)

WB (Western Blot)

(Western blot analysis of EpCAM on different cell lysates using anti- EpCAM antibody at 1/1000 dilution. Positive control: Lane1: Hela Lane2: NIH/3T3 Lane3: MCF-7)

Related Product Information for anti-EP-CAM antibody
Epithelial cell adhesion molecule (EpCAM) is a transmembrane glycoprotein mediating Ca2+-independent homotypic cell-cell adhesion in epithelia. EpCAM is also involved in cell signaling, migration, proliferation, and differentiation. Additionally, EpCAM has oncogenic potential via its capacity to upregulate c-myc, e-fabp, and cyclins A & E. Since EpCAM is expressed exclusively in epithelia and epithelial-derived neoplasms, EpCAM can be used as identification marker for various cancers. It appears to play a role in tumorigenesis and metastasis of carcinomas, so it can also act as a potential prognostic marker and as a potential target for immunotherapeutic strategies. Mutations in EpCAM have also been associated with congenital tufting enteropathy which causes intractable diarrhea in newborn children. Specificity/Source: This antibody is produced by immunizing mice with recombinant protein corresponding to a region of EpCAM.
Product Categories/Family for anti-EP-CAM antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
34,932 Da
NCBI Official Full Name
epithelial cell adhesion molecule
NCBI Official Synonym Full Names
epithelial cell adhesion molecule
NCBI Official Symbol
EPCAM
NCBI Official Synonym Symbols
ESA; KSA; M4S1; MK-1; DIAR5; EGP-2; EGP40; KS1/4; MIC18; TROP1; EGP314; HNPCC8; TACSTD1
NCBI Protein Information
epithelial cell adhesion molecule; epithelial glycoprotein 314; human epithelial glycoprotein-2; cell surface glycoprotein Trop-1; adenocarcinoma-associated antigen; tumor-associated calcium signal transducer 1; major gastrointestinal tumor-associated pro
UniProt Protein Name
Epithelial cell adhesion molecule
UniProt Gene Name
EPCAM
UniProt Synonym Gene Names
GA733-2; M1S2; M4S1; MIC18; TACSTD1; TROP1; Ep-CAM; EGP; EGP314; hEGP314
UniProt Entry Name
EPCAM_HUMAN

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Product Notes

The EP-CAM epcam (Catalog #AAA29891) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The EP-CAM Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's EP-CAM can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). WB: 1:1000-1:2000 IHC: 1:200-1:500 ICC: 1:100-1:200 FC/FACS: 1:100-1:200. Researchers should empirically determine the suitability of the EP-CAM epcam for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EP-CAM, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.