IF (Immunofluorescence)
(Immunofluorescence staining of Human EphB4 in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-Human EphB4 monoclonal antibody (FITC-conjugated, 10 ug/ml) at 37 degree C 1 hour. Positive staining was localized to plasma membrane.)
Mouse anti-Human EphB4 Monoclonal Antibody | anti-EphB4 antibody
Anti-EphB4 Antibody (FITC), Mouse Monoclonal
Gene Names
EPHB4; HTK; MYK1; TYRO11
Reactivity
Human
Applications
Immunofluorescence, Immunocytochemistry, Flow Cytometry, Functional Assay
Purity
Protein A
Synonyms
EphB4, Antibody; Anti-EphB4 Antibody (FITC), Mouse Monoclonal; EphB4 Antibody (FITC), Mouse MAb; EPH receptor B4; Anti-HTK Antibody; Anti-MYK1 Antibody; Anti-TYRO11 Antibody; anti-EphB4 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
Mouse IgG1
Clone Number
04
Specificity
Human EphB4
Purity/Purification
Protein A
Form/Format
Liquid; Aqueous solution containing 0.5% BSA and 0.09% sodium azide
Concentration
10ul/Test, 0.1mg/ml (varies by lot)
Applicable Applications for anti-EphB4 antibody
IF (Immunofluorescence), ICC (Immunocytochemistry), FCM/FACS (Flow Cytometry)
Immunogen
Recombinant Human EphB4 protein
Conjugation
FITC
Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human EphB4 (rhEphB4) and conjugated with FITC under optimum conditions, the unreacted FITC was removed.
Preparation and Storage
This antibody can be stored at 2-8 degree C for twelve months without detectable loss of activity. Protected from prolonged exposure to light. Do not freeze! Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal.
IF (Immunofluorescence)
(Immunofluorescence staining of Human EphB4 in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-Human EphB4 monoclonal antibody (FITC-conjugated, 10 ug/ml) at 37 degree C 1 hour. Positive staining was localized to plasma membrane.)
IF (Immunofluorescence)
(Immunofluorescence staining of Human EphB4 in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-Human EphB4 monoclonal antibody (FITC-conjugated, 10 ug/ml) at 37 degree C 1 hour. Positive staining was localized to plasma membrane.)
Application Data
(Profile of anti-EphB4 reactivity on MCF-7 cells analyzed by flow cytometry.)
Application Data
(Profile of anti-EphB4 reactivity on MCF-7 cells analyzed by flow cytometry.)
Related Product Information for anti-EphB4 antibody
Ephrin type-B receptor 4 is a protein that in humans is encoded by the EPHB4 gene. It is a single-pass type I membrane protein belonging to the ephrin receptor subfamily of protein kinase superfamily. Members of the ephrin and Eph family are local mediators of cell function through largely contact-dependent processes in development and in maturity. Furthermore, EphB4 protein and the corresponding ligand Ephrin-B2 contribute to tumor growth in various human tumors. EphB4 protein has tumor suppressor activities and that regulation of cell proliferation, extracellular matrix remodeling, and invasive potential are important mechanisms of tumor suppression. Therefore, Ephrin-B2/EphB4 may be recognized as a novel prognostic indicator for cancers.
Product Categories/Family for anti-EphB4 antibody
References
Davalos V, et al. (2006) EPHB4 and survival of colorectal cancer patients. Cancer Res. 66(18): 8943-8.Zhao C, et al. (2006) Bidirectional ephrinB2-EphB4 signaling controls bone homeostasis. Cell Metab. 4(2): 111-21.Kertesz N, et al. (2006) The soluble extracellular domain of EphB4 (sEphB4) antagonizes EphB4-EphrinB2 interaction, modulates angiogenesis, and inhibits tumor growth. Blood. 107(6): 2330-8.Noren NK, et al. (2007) Paradoxes of the EphB4 receptor in cancer. Cancer Res. 67(9): 3994-7.Taylor AC, et al. (2007) EphB4 expression along adult rat microvascular networks: EphB4 is more than a venous specific marker. Microcirculation. 14(3): 253-67.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
108,270 Da
NCBI Official Full Name
ephrin type-B receptor 4
NCBI Official Synonym Full Names
EPH receptor B4
NCBI Official Symbol
EPHB4
NCBI Official Synonym Symbols
HTK; MYK1; TYRO11
NCBI Protein Information
ephrin type-B receptor 4; ephrin receptor EphB4; soluble EPHB4 variant 1; soluble EPHB4 variant 2; soluble EPHB4 variant 3; hepatoma transmembrane kinase; tyrosine-protein kinase TYRO11; tyrosine-protein kinase receptor HTK
UniProt Protein Name
Ephrin type-B receptor 4
UniProt Gene Name
EPHB4
UniProt Synonym Gene Names
HTK; MYK1; TYRO11
UniProt Entry Name
EPHB4_HUMAN
Similar Products
Product Notes
The EphB4 ephb4 (Catalog #AAA254748) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-EphB4 Antibody (FITC), Mouse Monoclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's EphB4 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), ICC (Immunocytochemistry), FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the EphB4 ephb4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EphB4, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.