Application Data
(Fig.2: CGE analysis of purified GM1201 monoclonal antibody. Lane 1: molecular weight marker, Lane 2: 2 mug of purified GM1201 antibody. Proteins were separated by CGE (capillary gel electrophoresis, Agilent 2100 Bioanalyzer). Internal control bands (240 kDa / 7 kDa / 4,5 kDa).)
Epo-R Monoclonal Antibody | anti-EPOR antibody
Rat anti-human Epo-R monoclonal antibody
Gene Names
EPOR; EPO-R
Applications
Immunoprecipitation, Western Blot, Immunohistochemistry, ELISA, Flow Cytometry, Functional Assay
Purity
Protein G
Synonyms
Epo-R, Antibody; Rat anti-human Epo-R monoclonal antibody; anti-EPOR antibody
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
BCO-3H2
Specificity
anti-human Epo-R
Purity/Purification
Protein G
Sequence Length
508
Applicable Applications for anti-EPOR antibody
IP (Immunoprecipitation), WB (Western Blot), IHC (Immunohistochemistry), ELISA (Competitive ELISA), FCM/FACS (Flow Cytometry)
Buffer
Phosphate buffered saline, pH 7.2
Immunogen
Peptide immunization (GDSQGAQGGLSDGPYSN) from the cytoplasmic domain of human Epo-R
Selection
Based on recognition of the complete native protein expressed on mammalian cells and the denatured protein in Western blotting and immunohistochemistry
Preparation and Storage
Short term: 2-8°C
Long term:-20°C
(Avoid repeated freezing and thawing)
Long term:-20°C
(Avoid repeated freezing and thawing)
Application Data
(Fig.2: CGE analysis of purified GM1201 monoclonal antibody. Lane 1: molecular weight marker, Lane 2: 2 mug of purified GM1201 antibody. Proteins were separated by CGE (capillary gel electrophoresis, Agilent 2100 Bioanalyzer). Internal control bands (240 kDa / 7 kDa / 4,5 kDa).)
Application Data
(Fig.2: CGE analysis of purified GM1201 monoclonal antibody. Lane 1: molecular weight marker, Lane 2: 2 mug of purified GM1201 antibody. Proteins were separated by CGE (capillary gel electrophoresis, Agilent 2100 Bioanalyzer). Internal control bands (240 kDa / 7 kDa / 4,5 kDa).)
Application Data
(Fig.1: Mass spectroscopic analysis of EpoR immuno-precipitated from UT7 cells by GM-1201 or GM-1202 that were further analysed on denaturing SDS-PAGE gels (identified in Western blot with GM-1201). Segments of the gel were cut out for analysis by mass spectroscopy. For detailed information see Reference 5.)
Application Data
(Fig.1: Mass spectroscopic analysis of EpoR immuno-precipitated from UT7 cells by GM-1201 or GM-1202 that were further analysed on denaturing SDS-PAGE gels (identified in Western blot with GM-1201). Segments of the gel were cut out for analysis by mass spectroscopy. For detailed information see Reference 5.)
Related Product Information for anti-EPOR antibody
Purified Monoclonal Rat Antibody
Erythropoietin receptor (Epo-R) belongs to the cytokine receptor family and is a 507 amino acid type I transmembrane protein. EpoR pre-exists as dimers which changes the homodimerized state after binding of its 34 kDa ligand erythropoietin (Epo) (1,2). Erythropoietin is the primary regulator of erythropoiesis, and promotes the survival, proliferation, and differentiation of erythroid progenitor cells. Both, Epo and Epo-R are essential for the production of red blood cells due to Epo exerts its function through the Epo receptor (3). The Epo-R is also expressed in many organs outside the bone marrow, suggesting that Epo is a pleiotropic anti-apoptotic factor. Signaling pathways have been shown to influence numerous cellular functions in normal and tumor cells, including proliferation, apoptosis, and drug resistance (4). Development and specificity testing of the GM-1201 antibody are described in detail elsewhere (5).
Erythropoietin receptor (Epo-R) belongs to the cytokine receptor family and is a 507 amino acid type I transmembrane protein. EpoR pre-exists as dimers which changes the homodimerized state after binding of its 34 kDa ligand erythropoietin (Epo) (1,2). Erythropoietin is the primary regulator of erythropoiesis, and promotes the survival, proliferation, and differentiation of erythroid progenitor cells. Both, Epo and Epo-R are essential for the production of red blood cells due to Epo exerts its function through the Epo receptor (3). The Epo-R is also expressed in many organs outside the bone marrow, suggesting that Epo is a pleiotropic anti-apoptotic factor. Signaling pathways have been shown to influence numerous cellular functions in normal and tumor cells, including proliferation, apoptosis, and drug resistance (4). Development and specificity testing of the GM-1201 antibody are described in detail elsewhere (5).
References
1. Winkelmann JC (1992). The human erythropoietin receptor. Int J Cell Cloning. Sep;10(5):254-61
2. Livnah O, Stura EA, Middleton SA, Johnson DL, Jolliffe LK, Wilson IA (1999). Crystallographic evidence for preformed dimers of erythropoietin receptor before ligand activation. Science 283 (5404): 987-90
3. Wilson IA, Jolliffe LK (1999). The structure, organization, activation and plasticity of the erythropoietin receptor. Curr Opin Struct Biol.;9(6): 696-704
4. Hedley BD, Allan AL, Xenocostas A (2011). The role of erythropoietin and erythropoiesisstimulating agents in tumor progression. Clin Cancer Res. 15; 17(20): 6373-80
5. Maxwell P et al. (2015). Novel antibodies directed against the human erythropoietin receptor: creating a basis for clinical implementation. Br J Haematol; 168(3):429-42. doi: 10.1111/bjh.13133. Epub 2014 Oct 4.
2. Livnah O, Stura EA, Middleton SA, Johnson DL, Jolliffe LK, Wilson IA (1999). Crystallographic evidence for preformed dimers of erythropoietin receptor before ligand activation. Science 283 (5404): 987-90
3. Wilson IA, Jolliffe LK (1999). The structure, organization, activation and plasticity of the erythropoietin receptor. Curr Opin Struct Biol.;9(6): 696-704
4. Hedley BD, Allan AL, Xenocostas A (2011). The role of erythropoietin and erythropoiesisstimulating agents in tumor progression. Clin Cancer Res. 15; 17(20): 6373-80
5. Maxwell P et al. (2015). Novel antibodies directed against the human erythropoietin receptor: creating a basis for clinical implementation. Br J Haematol; 168(3):429-42. doi: 10.1111/bjh.13133. Epub 2014 Oct 4.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
erythropoietin receptor
NCBI Official Synonym Full Names
erythropoietin receptor
NCBI Official Symbol
EPOR
NCBI Official Synonym Symbols
EPO-R
NCBI Protein Information
erythropoietin receptor
UniProt Protein Name
Erythropoietin receptor
UniProt Gene Name
EPOR
UniProt Synonym Gene Names
EPO-R
UniProt Entry Name
EPOR_HUMAN
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Product Notes
The EPOR epor (Catalog #AAA71497) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Epo-R can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot), IHC (Immunohistochemistry), ELISA (Competitive ELISA), FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the EPOR epor for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Epo-R, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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