Application Data
(Fig.1: FACS analysis of BOSC23 cells using DP-4A1 Cat.# GM- 1001. BOSC23 cells were transiently transfected with an expression vector encoding either ErbB-1 (red curve) or an irrelevant protein (control transfectant: black curve). Binding of DP-4A1 was detected with a PE-conjugated secondary antibody. A positive signal was obtained only with ErbB-1 transfected cells.)
Mouse anti-Human ErbB1 Monoclonal Antibody | anti-EGFR antibody
anti-human Receptor tyrosine-protein kinase ErbB-1 monoclonal antibody
Gene Names
EGFR; ERBB; HER1; mENA; ERBB1; PIG61
Reactivity
Human
Applications
ELISA, Flow Cytometry, Functional Assay
Purity
Protein G
Synonyms
ErbB1, Antibody; anti-human Receptor tyrosine-protein kinase ErbB-1 monoclonal antibody; EGFR; anti-EGFR antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Clone Number
DP-4A1
Specificity
anti-human Receptor tyrosine-protein kinase ErbB-1 (EGFR)
Purity/Purification
Protein G
Form/Format
Purified
Sequence Length
1210
Applicable Applications for anti-EGFR antibody
ELISA (Competitive ELISA), FCM/FACS (Flow Cytometry)
Buffer
phosphate buffered saline, pH 7.2
Immunogen
genetic immunisation with cDNA encoding human ErbB-1
Selection
based on recognition of the complete native protein expressed on transfected
mammalian cells
Immunogen
cDNA
Preparation and Storage
Short term: 2 degree C - 8 degree C; long term: -20 degree C (avoid repeated freezing and thawing)
Application Data
(Fig.1: FACS analysis of BOSC23 cells using DP-4A1 Cat.# GM- 1001. BOSC23 cells were transiently transfected with an expression vector encoding either ErbB-1 (red curve) or an irrelevant protein (control transfectant: black curve). Binding of DP-4A1 was detected with a PE-conjugated secondary antibody. A positive signal was obtained only with ErbB-1 transfected cells.)
Application Data
(Fig.1: FACS analysis of BOSC23 cells using DP-4A1 Cat.# GM- 1001. BOSC23 cells were transiently transfected with an expression vector encoding either ErbB-1 (red curve) or an irrelevant protein (control transfectant: black curve). Binding of DP-4A1 was detected with a PE-conjugated secondary antibody. A positive signal was obtained only with ErbB-1 transfected cells.)
Application Data
(Fig.2: CGE analysis of purified DP-4A1 monoclonal antibody. Lane 1: molecular weight marker, Lane 2: 2 ?g of purified DP-4A1 antibody. Proteins were separated by CGE (capillary gel electrophoresis, Agilent 2100 Bioanalyzer). Internal control bands (240 kDa / 7 kDa / 4,5 kDa).)
Application Data
(Fig.2: CGE analysis of purified DP-4A1 monoclonal antibody. Lane 1: molecular weight marker, Lane 2: 2 ?g of purified DP-4A1 antibody. Proteins were separated by CGE (capillary gel electrophoresis, Agilent 2100 Bioanalyzer). Internal control bands (240 kDa / 7 kDa / 4,5 kDa).)
Related Product Information for anti-EGFR antibody
Background: Epidermal growth factor receptor (EGFR; ErbB-1) is a 170-kDa transmembrane glycoprotein encoded by the HER1 proto-oncogene. It belongs to the EGFR family of receptors, a subfamily of four closely related receptor tyrosine kinases: EGFR (ErbB-1), HER2/c-neu (ErbB-2), Her3 (ErbB-3) and Her4 (ErbB-4). ErbB-1 is expressed on the cell surface and is activated by binding of its specific ligands, including epidermal growth factor and transforming growth factor ? (TGF?). Growth factor binding to the extracellular domain of ErbB-1 induces formation of homoand heterodimers with another member of the ErbB receptor family. The EGFR signaling pathway is one of the most important pathways that regulate growth, survival, proliferation and differentiation in mammalian cells, while over-expression of ErbB-1 have been associated with a number of cancers, including lung cancer and glioblastoma (2,3).
References
Normanno N, Bianco C, Strizzi L, Mancino M, Maiello MR, De Luca A, Caponigro F and Salomon DS (2005). The ErbB receptors and their ligands in cancer: an overview. Curr Drug Targets 6(3):243-57
Harari PM (2004). Epidermal growth factor receptor inhibition strategies in oncology. Endocr Relat Cancer. 11(4):689-708
Harari PM (2004). Epidermal growth factor receptor inhibition strategies in oncology. Endocr Relat Cancer. 11(4):689-708
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
69,228 Da
NCBI Official Full Name
epidermal growth factor receptor isoform a
NCBI Official Synonym Full Names
epidermal growth factor receptor
NCBI Official Symbol
EGFR
NCBI Official Synonym Symbols
ERBB; HER1; mENA; ERBB1; PIG61
NCBI Protein Information
epidermal growth factor receptor; proto-oncogene c-ErbB-1; cell growth inhibiting protein 40; cell proliferation-inducing protein 61; receptor tyrosine-protein kinase erbB-1; avian erythroblastic leukemia viral (v-erb-b) oncogene homolog
UniProt Gene Name
EGFR
UniProt Synonym Gene Names
ERBB; ERBB1; HER1
UniProt Entry Name
EGFR_HUMAN
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Product Notes
The EGFR egfr (Catalog #AAA71483) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The anti-human Receptor tyrosine-protein kinase ErbB-1 monoclonal antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ErbB1 can be used in a range of immunoassay formats including, but not limited to, ELISA (Competitive ELISA), FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the EGFR egfr for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ErbB1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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