Application Data
(Fig.2: CGE analysis of purified DY-7G2 monoclonal antibody. Lane 1: molecular weight marker, Lane 2: 2 ?g of purified DY-7G2 antibody. Proteins were separated by CGE (capillary gel electrophoresis, Agilent 2100 Bioanalyzer). Internal control bands (240 kDa / 7 kDa / 4,5 kDa).)
Mouse anti-Human ErbB3 Monoclonal Antibody | anti-ERBB3 antibody
anti-human Receptor tyrosine-protein kinase ErbB-3 monoclonal antibody
Gene Names
ERBB3; HER3; LCCS2; ErbB-3; c-erbB3; erbB3-S; MDA-BF-1; c-erbB-3; p180-ErbB3; p45-sErbB3; p85-sErbB3
Reactivity
Human
Applications
Immunohistochemistry, Flow Cytometry, Functional Assay
Purity
Protein G
Synonyms
ErbB3, Antibody; anti-human Receptor tyrosine-protein kinase ErbB-3 monoclonal antibody; Her3; anti-ERBB3 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Clone Number
DY-7G2
Specificity
anti-human Receptor tyrosine-protein kinase ErbB-3 (Her3)
Purity/Purification
Protein G
Form/Format
Purified
Sequence Length
183
Applicable Applications for anti-ERBB3 antibody
IHC (Immunohistochemistry), FCM/FACS (Flow Cytometry)
Buffer
phosphate buffered saline, pH 7.2
Immunogen
genetic immunisation with cDNA encoding human ErbB-3
Selection
based on recognition of the complete native protein expressed on transfected mammalian cells
Immunogen
cDNA
Preparation and Storage
Short term: 2 degree C - 8 degree C; long term: -20 degree C (avoid repeated freezing and thawing)
Application Data
(Fig.2: CGE analysis of purified DY-7G2 monoclonal antibody. Lane 1: molecular weight marker, Lane 2: 2 ?g of purified DY-7G2 antibody. Proteins were separated by CGE (capillary gel electrophoresis, Agilent 2100 Bioanalyzer). Internal control bands (240 kDa / 7 kDa / 4,5 kDa).)
Application Data
(Fig.2: CGE analysis of purified DY-7G2 monoclonal antibody. Lane 1: molecular weight marker, Lane 2: 2 ?g of purified DY-7G2 antibody. Proteins were separated by CGE (capillary gel electrophoresis, Agilent 2100 Bioanalyzer). Internal control bands (240 kDa / 7 kDa / 4,5 kDa).)
Application Data
(Fig.1: FACS analysis of BOSC23 cells using DY-7G2 Cat.# GM- 1003. BOSC23 cells were transiently transfected with an expression vector encoding either ErbB-3 (red curve) or an irrelevant protein (control transfectant: black curve). Binding of DY-7G2 was detected with a PE-conjugated secondary antibody. A positive signal was obtained only with ErbB-3 transfected cells.)
Application Data
(Fig.1: FACS analysis of BOSC23 cells using DY-7G2 Cat.# GM- 1003. BOSC23 cells were transiently transfected with an expression vector encoding either ErbB-3 (red curve) or an irrelevant protein (control transfectant: black curve). Binding of DY-7G2 was detected with a PE-conjugated secondary antibody. A positive signal was obtained only with ErbB-3 transfected cells.)
Related Product Information for anti-ERBB3 antibody
Background: ErbB-3 belongs to the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases. ErbB receptors (EGFR (ErbB1), ErbB2, ErbB3, and ErbB4) are important regulators of normal growth and differentiation, and they are involved in the pathogenesis of cancer. The 148- kDa trans-membrane polypeptide ErbB-3 has a neuregulin binding domain but not an active kinase domain. Heterodimerisation with EGF receptor family members which possess kinase activity leads to the activation of pathways which indicate cell proliferation or differentiation (1). Over-expression of ErbB-3 has been reported in numerous cancers, including prostate, bladder and breast tumours (2).
References
Kraus MH, Issing W, Miki T, Popescu NC, Aaronson SA (1989). Isolation and characterization of ERBB3, a third member of the ERBB/epidermal growth factor receptor family: evidence for overexpression in a subset of human mammary tumors. Proc Natl Acad Sci USA, 86(23):9193-7
Gullick WJ (1996). The c-erbB3/HER3 receptor in human cancer. Cancer Surv 27:339-49.
Gullick WJ (1996). The c-erbB3/HER3 receptor in human cancer. Cancer Surv 27:339-49.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
77,426 Da
NCBI Official Full Name
receptor tyrosine-protein kinase erbB-3 isoform s
NCBI Official Synonym Full Names
v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 3
NCBI Official Symbol
ERBB3
NCBI Official Synonym Symbols
HER3; LCCS2; ErbB-3; c-erbB3; erbB3-S; MDA-BF-1; c-erbB-3; p180-ErbB3; p45-sErbB3; p85-sErbB3
NCBI Protein Information
receptor tyrosine-protein kinase erbB-3; proto-oncogene-like protein c-ErbB-3; tyrosine kinase-type cell surface receptor HER3
UniProt Gene Name
ERBB3
UniProt Synonym Gene Names
HER3
UniProt Entry Name
ERBB3_HUMAN
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Product Notes
The ERBB3 erbb3 (Catalog #AAA71484) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The anti-human Receptor tyrosine-protein kinase ErbB-3 monoclonal antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ErbB3 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the ERBB3 erbb3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ErbB3, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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