FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of 2% paraformaldehyde-fixed Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling FAF2 with M25099 at 1/200 dilution (red) compared with a mouse monoclonal IgG isotype control (black) and an unlabelled control (cells without incubation with primary antibody, blue). Goat anti-mouse IgG (FITC) at 1/300 dilution was used as the secondary antibody.)
Mouse anti-Human ETEA Monoclonal Antibody | anti-ETEA antibody
ETEA Mouse mAb
Gene Names
FAF2; ETEA; UBXD8; UBXN3B
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Immunoprecipitation, Western Blot
Synonyms
ETEA, Antibody; ETEA Mouse mAb; expressed in T-cells and eosinophils in atopic dermatitis; FAF2; Fas associated factor family member 2; FAS-associated factor 2; KIAA0887; Protein ETEA; protein expressed in T-cells and eosinophils in atopic dermatitis; UBX domain containing 8; UBX domain protein 3B; UBX domain-containing protein 3B; UBX domain-containing protein 8; UBXD8; UBXN3B; 2210404D11Rik; AI462440; ETEA; mKIAA0887; anti-ETEA antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG
Sequence Length
445
Applicable Applications for anti-ETEA antibody
FCM/FACS (Flow Cytometry), IP (Immunoprecipitation), WB (Western Blot)
Source Note
This monoclonal antibody is produced by immunizing mice with a sythetic peptide(KLH coupled)corresponding to amino-terminal residues of human FAF2
Preparation and Storage
Store at -20 degree C. Stable for one year from the date of shipment.
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of 2% paraformaldehyde-fixed Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling FAF2 with M25099 at 1/200 dilution (red) compared with a mouse monoclonal IgG isotype control (black) and an unlabelled control (cells without incubation with primary antibody, blue). Goat anti-mouse IgG (FITC) at 1/300 dilution was used as the secondary antibody.)
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of 2% paraformaldehyde-fixed Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling FAF2 with M25099 at 1/200 dilution (red) compared with a mouse monoclonal IgG isotype control (black) and an unlabelled control (cells without incubation with primary antibody, blue). Goat anti-mouse IgG (FITC) at 1/300 dilution was used as the secondary antibody.)
IP (Immunoprecipitation)
(FAF2 was immunoprecipitated from 1mg of Jurkat cells membrane fraction, blotted with M25099 of 10 ug. Western blot was performed from the immunoprecipitate using M25099 at 1/1000 dilution. Anti-Mouse-IgG(HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/10000 dilution. Lane 1: Jurkat cells membrane fraction. Lane2: IP product of Jurkat cells membrane fraction.Predicted band size : 53 KDaObserved band size : 53 KDaBlocking and dilution buffer and concentration:5% milk/TBST.)
IP (Immunoprecipitation)
(FAF2 was immunoprecipitated from 1mg of Jurkat cells membrane fraction, blotted with M25099 of 10 ug. Western blot was performed from the immunoprecipitate using M25099 at 1/1000 dilution. Anti-Mouse-IgG(HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/10000 dilution. Lane 1: Jurkat cells membrane fraction. Lane2: IP product of Jurkat cells membrane fraction.Predicted band size : 53 KDaObserved band size : 53 KDaBlocking and dilution buffer and concentration:5% milk/TBST.)
WB (Western Blot)
WB (Western Blot)
Related Product Information for anti-ETEA antibody
The protein encoded by this gene is highly expressed in peripheral blood of patients with atopic dermatitis (AD), compared to normal individuals. It may play a role in regulating the resistance to apoptosis that is observed in T cells and eosinophils of AD patients.
Product Categories/Family for anti-ETEA antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Predicted MW: 53kDa
NCBI Official Full Name
FAS-associated factor 2
NCBI Official Synonym Full Names
Fas associated factor family member 2
NCBI Official Symbol
FAF2
NCBI Official Synonym Symbols
ETEA; UBXD8; UBXN3B
NCBI Protein Information
FAS-associated factor 2
UniProt Protein Name
FAS-associated factor 2
UniProt Gene Name
FAF2
UniProt Synonym Gene Names
ETEA; KIAA0887; UBXD8; UBXN3B
Similar Products
Product Notes
The ETEA faf2 (Catalog #AAA62382) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The ETEA Mouse mAb reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ETEA can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the ETEA faf2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ETEA, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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