Mouse Ezrin/p81 Monoclonal Antibody | anti-Ezrin antibody

Ezrin/p81

Cat. Num. AAA214832

• Gene Names: EZR; CVL; CVIL; VIL2; HEL-S-105
• Reactivity: Human. Others not tested.
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Synonyms: Ezrin/p81, Antibody; Ezrin/p81; Villin-2; CVIL; Epididymis secretory protein Li 105; EZR; p81; VIL2; Cytovillin; Cytovillin 2; HEL S 105; DKFZp762H157; anti-Ezrin antibody

• Host: Mouse
• Reactivity: Human. Others not tested.
• Clonality: Monoclonal
• Isotype: IgG2b
• Clone Number: CPTC-Ezrin-1
• Specificity: Ezrin, Moesin and Radixin belong to a family of highly homologous Actinassociated proteins that are localized just beneath the plasma membrane. The proteins are believed to be involved in the mediation of interactions between cytoskeletal and membrane proteins. Ezrin serves as a major cytoplasmic substrate of various protein-tyrosine kinases, including the epidermal growth factor receptor. Ezrin has also been identified as a cAMP-dependent protein kinase (A-kinase) anchoring protein and designated AKAP78. Moesin and Radixin share over 70% homology with Ezrin and are coexpressed within various cell types. Despite the high degree of homology, the three proteins exhibit a distinct receptor-specific pattern of phosphorylation. Overexpression of Ezrin predicts the poor prognosis of gastric adenocarcinoma.
• Purity/Purification: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Form/Format: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 586

• Applicable Applications for anti-Ezrin antibody: IHC (Immunohistochemistry), WB (Western Blot)
• Immunogen: Recombinant human full-length Ezrin protein
• Positive Control: SKBr3, HeLa, HEK293, MCF-7, Jurkat, A431, MCF-7. Cerebral cortex and hippocampus.
• Cellular Localization: Cell Surface.
• Chromosome Location: 6q25.3
• Preparation and Storage: Antibody with azide - store at 2 to 8 degree C.; Antibody without azide - store at -20 to -80 degree C.; Antibody is stable for 24 months. Non-hazardous. No MSDS required.

WB (Western Blot)

SDS-PAGE

Application Data

(Analysis of Protein Array containing more than 19,000 full-length human proteins using Ezrin/p81 Mouse Monoclonal Antibody (CPTC-Ezrin-1). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)

IHC (Immunohistochemistry)

(Formalin-fixed, paraffin-embedded human Placenta stained with Ezrin Mouse Monoclonal Antibody (CPTC-Ezrin-1).)

References

Jin, Jingchun, et al. Pathology, BioMed Central, 5 Oct. 201

NCBI and Uniprot Product Information

• NCBI GI #: 161702986
• NCBI GeneID: 7430
• NCBI Accession #: NP_001104547.1
• NCBI GenBank Nucleotide #: NM_001111077.1
• UniProt Accession #: P15311; P23714; Q4VX75; Q96CU8; Q9NSJ4; E1P5A8
• Molecular Weight: 87kDa
• NCBI Official Full Name: ezrin
• NCBI Official Synonym Full Names: ezrin
• NCBI Official Symbol: EZR
• NCBI Official Synonym Symbols: CVL; CVIL; VIL2; HEL-S-105
• NCBI Protein Information: ezrin
• UniProt Protein Name: Ezrin
• UniProt Gene Name: EZR
• UniProt Synonym Gene Names: VIL2