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Mouse anti-Human F2 Monoclonal Antibody | anti-F2 antibody
F2 (Coagulation Factor II, Prothrombin) APC
Gene Names
F2; PT; THPH1; RPRGL2
Reactivity
Human
Applications
ELISA, Immunoprecipitation, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
F2, Antibody; F2 (Coagulation Factor II, Prothrombin) APC; anti-F2 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG
Clone Number
1B7
Specificity
Recognizes human F2.
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
Applicable Applications for anti-F2 antibody
ELISA (EIA), Immunoprecipitation (IP), Western Blot (WB)
Application Notes
Applications are based on unconjugated antibody.
Immunogen
Partial recombinant corresponding to aa263-362 from human F2 (AAH51332) with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
RNPDGDEEGAWCYVAGKPGDFGYCDLNYCEEAVEEETGDGLDEDSDRAIEGRTATSEYQTFFNPRTFGSGEADCGLRPLFEKKSLEDKTERELLESYIDG
Conjugate
APC
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Application Data
(Proximity Ligation Analysis (PLA) of protein-protein interactions between FGA and F2. HeLa cells were stained with FGA rabbit purified polyclonal 1:1200 and F2 mouse monoclonal antibody 1:50. Signals were detected by 30 Detection Kit 613 (red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.)
WB (Western Blot)
(Western blot analysis of F2 over-expressed 293 cell line, cotransfected with F2 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with F2 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)
Application Data
(Detection limit for recombinant GST tagged F2 is ~1ng/ml as a capture antibody.)
IP (Immunoprecipitation)
(Immunoprecipitation of F2 transfected lysate using F2 monoclonal antibody and Protein A Magnetic Bead and immunoblotted with F2 rabbit polyclonal antibody.)
WB (Western Blot)
(Western Blot analysis of F2 expression in transfected 293T cell line by F2 monoclonal antibody. Lane 1: F2 transfected lysate (70kD). Lane 2: Non-transfected lysate.)
WB (Western Blot)
(Western Blot detection against Immunogen (36.74kD).)
Related Product Information for anti-F2 antibody
Thrombin, which cleaves bonds after Arg and Lys, converts fibrinogen to fibrin and activates factors V, VII, VIII, XIII, and, in complex with thrombomodulin, protein C. Functions in blood homeostasis, inflammation and wound healing.
Product Categories/Family for anti-F2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
70,037 Da
NCBI Official Full Name
Homo sapiens coagulation factor II (thrombin), mRNA
NCBI Official Synonym Full Names
coagulation factor II (thrombin)
NCBI Official Symbol
F2
NCBI Official Synonym Symbols
PT; THPH1; RPRGL2
NCBI Protein Information
prothrombin; serine protease; prothrombin B-chain; prepro-coagulation factor II
UniProt Protein Name
Prothrombin
UniProt Gene Name
F2
UniProt Entry Name
THRB_HUMAN
