Rat anti-Mouse F4/80 Monoclonal Antibody | anti-F4/80 antibody

RAT ANTI MOUSE F4/80:FITC

Cat. Num. AAA49693

• Gene Names: Emr1; Ly71; F4/80; Gpf480; TM7LN3; DD7A5-7; EGF-TM7
• Reactivity: Mouse
• Applications: Immunofluorescence, Flow Cytometry, Functional Assay
• Synonyms: F4/80, Antibody; RAT ANTI MOUSE F4/80:FITC; Gpf480; anti-F4/80 antibody

• Host: Rat
• Reactivity: Mouse
• Clonality: Monoclonal
• Isotype: IgG2b
• Clone Number: A3-1
• Specificity: Rat anti mouse F4/80 antibody, clone Cl:A3-1 recognises the murine F4/80 antigen, a ~160 kDa cell surface glycoprotein member of the EGF-TM7 family of proteins which shares 68% overall amino acid identity with human EGF module-containing mucin-like hormone receptor 1 (EMR1).; Expression of F4/80 is heterogeneous and is modulated during macrophage maturation and activation. The F4/80 antigen is expressed on a wide range of mature tissue macrophages including Kupffer cells, Langerhans cells, microglia, macrophages located in the gut lamina propria, peritoneal cavity, lung, thymus, bone marrow stroma and macrophages in the red pulp of the spleen (Hume, et al. 1984). F4/80 antigen is also expressed on a subpopulation of dendritic cells but is absent from macrophages located in T cell areas of the spleen and lymph node (Gordon, et al. 1994). The ligands and biological functions of the F4/80 antigen have not been fully determined but a role for F4/80 in the generation of efferent CD8+ve regulatory T cells is proposed (Lin, et al. 2005); Rat anti mouse F4/80 antibody, clone Cl:A3-1 modulates cytokine levels released in response to Listeria monocytogenes (Warschkau & Kiderlen, 1999).; A Human anti-idiotypic CI:A31 antibody, clone 17867 (HCA154 ) which binds to and blocks activity of Rat anti mouse F4/80 antibody, clone Cl:A3-1 is also available for use as a control in experiments utilizing clone A3-1.
• Form/Format: Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
• Concentration: IgG concentration 0.1 mg/ml (varies by lot)

• Applicable Applications for anti-F4/80 antibody: IF (Immunofluorescence), FCM/FACS (Flow Cytometry)
• Target Species: Mouse
• Max Ex/Em: Fluorophore: FITC; Excitation Max (nm): 490; Emission Max (nm): 525
• Preparation: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
• Buffer Solution: Phosphate buffered saline
• Preservative Stabilizers: 0.09% Sodium Azide; 1% Bovine Serum Albumin
• Immunogen: Thioglycollate stimulated peritoneal macrophages from C57BL/6 mice.
• Fusion Partners: Spleen cells from immunised HOB2 rats were fused with cells of the mouse NS1 myeloma cell line.
• Preparation and Storage: Store at +4°C or at -20°C if preferred.; This product should be stored undiluted.; Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.; Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

NCBI and Uniprot Product Information

• NCBI GI #: 33859546
• NCBI GeneID: 13733
• NCBI Accession #: NP_034260.1
• NCBI GenBank Nucleotide #: NM_010130.4
• UniProt Accession #: Q61549
• NCBI Official Full Name: EGF-like module-containing mucin-like hormone receptor-like 1
• NCBI Official Synonym Full Names: EGF-like module containing, mucin-like, hormone receptor-like sequence 1
• NCBI Official Symbol: Emr1
• NCBI Official Synonym Symbols: Ly71; F4/80; Gpf480; TM7LN3; DD7A5-7; EGF-TM7
• NCBI Protein Information: EGF-like module-containing mucin-like hormone receptor-like 1; EGF-like module receptor 1; EMR1 hormone receptor; cell surface glycoprotein F4/80; lymphocyte antigen 71
• UniProt Protein Name: EGF-like module-containing mucin-like hormone receptor-like 1
• UniProt Gene Name: Emr1
• UniProt Synonym Gene Names: Gpf480
• UniProt Entry Name: EMR1_MOUSE