<
FCM (Flow Cytometry)
(Flow cytometric analysis of Hela cells with Fascin antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).)
Rabbit Fascin Monoclonal Antibody | anti-FSCN1 antibody
Fascin Antibody
Gene Names
FSCN1; HSN; SNL; p55; FAN1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Flow Cytometry, Functional Assay
Purity
ProA affinity purified
Synonyms
Fascin; Monoclonal Antibody; Fascin Antibody; 55 kDa actin bundling protein antibody; 55 kDa actin-bundling protein antibody; Actin bundling protein antibody; actin bundling protein; 55-KD antibody; FAN 1 antibody; FAN1 antibody; Fascin 1 antibody; Fascin actin bundling protein 1 antibody; Fascin antibody; Fascin homolog 1 actin bundling protein (Strongylocentrotus purpuratus) antibody; Fascin homolog 1 antibody; sea urchin; homolog of; 1 antibody; Fascin1 antibody; FLJ38511 antibody; FSCN 1 antibody; FSCN1 antibody; FSCN1_HUMAN antibody; HSN antibody; p55 antibody; Singed (Drosophila) like (sea urchin fascin homolog like) antibody; Singed drosophila homolog like antibody; Singed like (fascin homolog sea urchin) (Drosophila) antibody; Singed like (fascin homolog sea urchin) antibody; Singed like protein antibody; Singed; drosophila; homolog of antibody; Singed-like protein antibody; SNL antibody; Strongylocentrotus purpuratus antibody; anti-FSCN1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Clone Number
JM12-53
Purity/Purification
ProA affinity purified
Form/Format
1*TBS (pH7.4), 1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Applicable Applications for anti-FSCN1 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Flow Cytometry (FC/FACS)
Application Notes
WB: 1:500-1:1000
IHC: 1:50-1:200
ICC: 1:50-1:200
FC/FACS: 1:50-1:100
IHC: 1:50-1:200
ICC: 1:50-1:200
FC/FACS: 1:50-1:100
Antibody Type
Recombinant Antibody
Immunogen
Recombinant protein
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C.
FCM (Flow Cytometry)
(Flow cytometric analysis of Hela cells with Fascin antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).)
FCM (Flow Cytometry)
(Flow cytometric analysis of Hela cells with Fascin antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).)
ICC (Immunocytochemistry)
(ICC staining Fascin in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining Fascin in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining Fascin in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining Fascin in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining Fascin in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining Fascin in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Fascin antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Fascin antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Fascin antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Fascin antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-Fascin antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-Fascin antibody. Counter stained with hematoxylin.)
WB (Western Blot)
(Western blot analysis of Fascin on different lysates using anti-Fascin antibody at 1/1, 000 dilution. Positive control�� Lane1: SH-SY5Y Lane2: Human brain Lane3: Rat spleen Lane4: Mouse testes Lane5: Mouse brain Lane6: Mouse spleen)
WB (Western Blot)
(Western blot analysis of Fascin on different lysates using anti-Fascin antibody at 1/1, 000 dilution. Positive control�� Lane1: SH-SY5Y Lane2: Human brain Lane3: Rat spleen Lane4: Mouse testes Lane5: Mouse brain Lane6: Mouse spleen)
Related Product Information for anti-FSCN1 antibody
Cell adhesion to extracellular matrix is an important physiological stimulus for organization of the Actin-based cytoskeleton. Adhesion to the matrix glycoprotein Thrombospondin 1 triggers the sustained formation of F-Actin microspikes that contain the Actin-bundling protein Fascin. These structures are also implicated in cell migration, which may be an important function of Thrombospondin 1 in tissue remodelling and wound repair. Fascin bundles Actin microfilaments within dynamic cellular structures such as microspikes, stress fibers and membrane ruffles. Fascin could serve as a prognostic factor for abnormal ovarian epithelial pathology and could be a novel target for the treatment of ovarian cancer. Fascin, an Actin-bundling protein, identifies dendritic cells in the blood and in tissues.
Product Categories/Family for anti-FSCN1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
Calculated MW: 54kDa
Observed MW: 50kDa, 55kDa
Observed MW: 50kDa, 55kDa
NCBI Official Full Name
Fascin
NCBI Official Synonym Full Names
fascin actin-bundling protein 1
NCBI Official Symbol
FSCN1
NCBI Official Synonym Symbols
HSN; SNL; p55; FAN1
NCBI Protein Information
fascin; 55 kDa actin-bundling protein; fascin homolog 1, actin-bundling protein; singed-like (fascin homolog, sea urchin)
UniProt Protein Name
Fascin
UniProt Gene Name
FSCN1
UniProt Synonym Gene Names
FAN1; HSN; SNL
UniProt Entry Name
FSCN1_HUMAN
Similar Products
Product Notes
The FSCN1 fscn1 (Catalog #AAA30366) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Fascin Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Fascin can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Flow Cytometry (FC/FACS). WB: 1:500-1:1000 IHC: 1:50-1:200 ICC: 1:50-1:200 FC/FACS: 1:50-1:100. Researchers should empirically determine the suitability of the FSCN1 fscn1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Fascin, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
