ICC (Immunocytochemistry)
(Immunocytochemical labeling of fascin phosphorylation relative to F-actin in chick E9 DRG neurons. The cells were labeled with rabbit polyclonal Fascin (Ser-39) antibody, then detected using appropriate secondary antibody (Red). Fascin (Ser-39) labeling is compared (Top) to F-actin staining (Green). (Image provided by Dr. Gianluca Gallo at Drexel University).)
Rabbit Fascin Polyclonal Antibody | anti-FSCN1 antibody
Fascin (Ser-39), phospho-specific Antibody
Gene Names
FSCN1; HSN; SNL; p55; FAN1
Reactivity
Human, Rat, Mouse, Chicken
Applications
Immunocytochemistry, ELISA, Western Blot
Synonyms
Fascin, Antibody; Fascin (Ser-39), phospho-specific Antibody; Fascin (Ser-39), phospho-specific; Fascin 039 pAb; anti-FSCN1 antibody
Host
Rabbit
Reactivity
Human, Rat, Mouse, Chicken
Clonality
Polyclonal
Specificity
This antibody was cross-adsorbed to a phospho-serine peptide before affinity purified using phosho-fascin (Ser-39) peptide (without carrier). The antibody detects a 55kDa* band corresponding to fascin on SDS-PAGE immunoblots of mouse C2C12 and human HeLa cells treated with Calyculin A. This band is not observed after lambda phosphatase treatment. The antibody also detects fascin (Ser-39) phosphorylation in chick DRG neurons.
Form/Format
Rabbit polyclonal, affinity-purified antibody is supplied in 100ul phosphate-buffered saline, 50% glycerol, 1mg/ml BSA, and 0.05% sodium azide.
Applicable Applications for anti-FSCN1 antibody
ICC (Immunocytochemistry), ELISA, WB (Western Blot)
Immunogen
Fascin (Ser-39) synthetic peptide (coupled to carrier protein) corresponds to amino acids surrounding serine 39 in human fascin. This sequence has high homology to the conserved site in rat and mouse fascin, as well as to the conserved region in fascin-2, but has low homology to the conserved region in fascin-3.
Preparation and Storage
Store at -20 degree C. Stable for 1 year.
ICC (Immunocytochemistry)
(Immunocytochemical labeling of fascin phosphorylation relative to F-actin in chick E9 DRG neurons. The cells were labeled with rabbit polyclonal Fascin (Ser-39) antibody, then detected using appropriate secondary antibody (Red). Fascin (Ser-39) labeling is compared (Top) to F-actin staining (Green). (Image provided by Dr. Gianluca Gallo at Drexel University).)
ICC (Immunocytochemistry)
(Immunocytochemical labeling of fascin phosphorylation relative to F-actin in chick E9 DRG neurons. The cells were labeled with rabbit polyclonal Fascin (Ser-39) antibody, then detected using appropriate secondary antibody (Red). Fascin (Ser-39) labeling is compared (Top) to F-actin staining (Green). (Image provided by Dr. Gianluca Gallo at Drexel University).)
WB (Western Blot)
(Western blot analysis of human HeLa cells treated with Calyculin A (100nM) for 30 min (lanes 1 & 3) before treatment with lambda phosphatase (lanes 2 & 4). The blots were probed with anti-Fascin (clone 55K2) (lanes 1 & 2) and anti-Fascin (Ser-39) (lanes 3 & 4).)
WB (Western Blot)
(Western blot analysis of human HeLa cells treated with Calyculin A (100nM) for 30 min (lanes 1 & 3) before treatment with lambda phosphatase (lanes 2 & 4). The blots were probed with anti-Fascin (clone 55K2) (lanes 1 & 2) and anti-Fascin (Ser-39) (lanes 3 & 4).)
Related Product Information for anti-FSCN1 antibody
Fascin is an actin filament bundling protein localized to lamellipodia and filopodia where it has important roles in cell motility. Regulation of fascin occurs through PKC-mediated phosphorylation of Ser-39 in the F-actin binding site. Cell permeant peptides that block PKC phosphorylation of Ser-39 increase cell migration, while peptides that block fascin binding to F-actin alter lamellipodial morphology and cause aberrant cell motility. Studies using RNA interference of fascin show that fibroblasts have reduced number and abnormal morphology of filopodia, while Ser-39 phosphorylation status may determine filopodial frequency. In Drosophila neurons, fascin deficiency causes alterations in actin filaments and leads to abnormal morphology of developing neurons. Thus, fascin is a critical element of actin-based motility in various cell types.
References
Aratyn, Y.S. et al. (2007) Mol Biol Cell. 18(10):3928.
Kraft, R. et al. (2006) J Neurosci 26:8734.
Vignjevic, D. et al. (2006) J Cell Biol. 174(6):863.
Adams, J.C. (2004) Curr Opin Cell Biol. 16(5):590.
Kraft, R. et al. (2006) J Neurosci 26:8734.
Vignjevic, D. et al. (2006) J Cell Biol. 174(6):863.
Adams, J.C. (2004) Curr Opin Cell Biol. 16(5):590.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
Calculated MW: 54kDa
Observed MW: 50kDa, 55kDa
Observed MW: 50kDa, 55kDa
NCBI Official Full Name
Fascin
NCBI Official Synonym Full Names
fascin actin-bundling protein 1
NCBI Official Symbol
FSCN1
NCBI Official Synonym Symbols
HSN; SNL; p55; FAN1
NCBI Protein Information
fascin; 55 kDa actin-bundling protein; fascin homolog 1, actin-bundling protein; singed-like (fascin homolog, sea urchin)
UniProt Protein Name
Fascin
UniProt Gene Name
FSCN1
UniProt Synonym Gene Names
FAN1; HSN; SNL
UniProt Entry Name
FSCN1_HUMAN
Similar Products
Product Notes
The FSCN1 fscn1 (Catalog #AAA71641) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Fascin (Ser-39), phospho-specific Antibody reacts with Human, Rat, Mouse, Chicken and may cross-react with other species as described in the data sheet. AAA Biotech's Fascin can be used in a range of immunoassay formats including, but not limited to, ICC (Immunocytochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the FSCN1 fscn1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Fascin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.