Application Data
(SDS-PAGE analysis of GM-5F4 The antibody was purified by protein G affinity chromatography from cell culture supernatants and verified by SDS-Page (Fig.2). Fig.2: SDS-PAGE analysis of purified GM-5F4 monoclonal antibody. Lane 1: molecular weight marker, Lane 2: 2 ?g of purified GM-5F4 antibody. Proteins were separated by SDSPAGE and stained with RAPID StainTM Reagent.)
Mouse anti-Human FASL Monoclonal Antibody | anti-FASLG antibody
anti-human FAS ligand (FasL) monoclonal antibody
Gene Names
FASLG; APTL; FASL; CD178; CD95L; ALPS1B; CD95-L; TNFSF6; APT1LG1
Reactivity
Human
Applications
ELISA, Flow Cytometry, Functional Assay
Purity
Protein G
Synonyms
FASL, Antibody; anti-human FAS ligand (FasL) monoclonal antibody; CD95; Fas ligand; anti-FASLG antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1/kappa
Clone Number
GM-5F4
Specificity
anti-human Fas ligand (FasL)
Purity/Purification
Protein G
Form/Format
Purified
Sequence Length
281
Applicable Applications for anti-FASLG antibody
ELISA, FCM/FACS (Flow Cytometry)
Buffer
phosphate buffered saline, pH 7.2
Immunogen
genetic immunisation with cDNA encoding human FasL (extracellular domain)
Selection
based on recognition of the complete native protein expressed on transfected mammalian cells
Immunogen
cDNA
Preparation and Storage
Short term: 2 degree C - 8 degree C; long term: -20 degree C (avoid repeated freezing and thawing)
Application Data
(SDS-PAGE analysis of GM-5F4 The antibody was purified by protein G affinity chromatography from cell culture supernatants and verified by SDS-Page (Fig.2). Fig.2: SDS-PAGE analysis of purified GM-5F4 monoclonal antibody. Lane 1: molecular weight marker, Lane 2: 2 ?g of purified GM-5F4 antibody. Proteins were separated by SDSPAGE and stained with RAPID StainTM Reagent.)
Application Data
(SDS-PAGE analysis of GM-5F4 The antibody was purified by protein G affinity chromatography from cell culture supernatants and verified by SDS-Page (Fig.2). Fig.2: SDS-PAGE analysis of purified GM-5F4 monoclonal antibody. Lane 1: molecular weight marker, Lane 2: 2 ?g of purified GM-5F4 antibody. Proteins were separated by SDSPAGE and stained with RAPID StainTM Reagent.)
Application Data
(Fig.1: FACS analysis of BOSC23 cells using GM-5F4. cells were transiently transfected with an expression vector encoding either FasL (red curve) or an irrelevant protein (control transfectant: black curve). Binding of GM-5F4 was detected with a PE-conjugated secondary antibody. A positive signal was obtained only with FasL transfected cells.)
Application Data
(Fig.1: FACS analysis of BOSC23 cells using GM-5F4. cells were transiently transfected with an expression vector encoding either FasL (red curve) or an irrelevant protein (control transfectant: black curve). Binding of GM-5F4 was detected with a PE-conjugated secondary antibody. A positive signal was obtained only with FasL transfected cells.)
Related Product Information for anti-FASLG antibody
Background: The FAS ligand (FASL, CD95), a member of the tumor necrosis factor family, induces apoptosis in FAS-bearing cells (1). FASL is a type II membrane receptor with a soluble form that can be released into the extracellular fluid by proteolytic processing. Various cells express Fas, whereas FASL is expressed in activated splenocytes and thymocytes (2). In the immune system, Fas and FasL are involved in down-regulation of immune reactions as well as in T cellmediated cytotoxicity. Malfunction of the Fas system causes lymphoproliferative disorders and accelerates autoimmune diseases, whereas its exacerbation may cause tissue destruction.
References
Nagata S (1996). Apoptosis mediated by the Fas system. Prog Mol Subcell Biol 16:87-103
Suda T,Hashimoto H, Tanaka M, Ochi T, Nagata S (1997). Membrane Fas ligand kills human peripheral blood T lymphocytes, and soluble Fas ligand blocks the killing. J Exp Med 186(12):2045-50
Nagata S and Golstein P (1995). The Fas death factor. Science 10;267(5203):1449-56
Suda T,Hashimoto H, Tanaka M, Ochi T, Nagata S (1997). Membrane Fas ligand kills human peripheral blood T lymphocytes, and soluble Fas ligand blocks the killing. J Exp Med 186(12):2045-50
Nagata S and Golstein P (1995). The Fas death factor. Science 10;267(5203):1449-56
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
tumor necrosis factor ligand superfamily member 6
NCBI Official Synonym Full Names
Fas ligand (TNF superfamily, member 6)
NCBI Official Symbol
FASLG
NCBI Official Synonym Symbols
APTL; FASL; CD178; CD95L; ALPS1B; CD95-L; TNFSF6; APT1LG1
NCBI Protein Information
tumor necrosis factor ligand superfamily member 6; CD95 ligand; fas antigen ligand; apoptosis antigen ligand; apoptosis (APO-1) antigen ligand 1; tumor necrosis factor (ligand) superfamily, member 6
UniProt Gene Name
FASLG
UniProt Synonym Gene Names
APT1LG1; CD95L; FASL; TNFSF6; APTL; CD95-L; Fas ligand; FasL; sFasL; APL; FasL ICD; SPA
UniProt Entry Name
TNFL6_HUMAN
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Product Notes
The FASLG faslg (Catalog #AAA71472) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The anti-human FAS ligand (FasL) monoclonal antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's FASL can be used in a range of immunoassay formats including, but not limited to, ELISA, FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the FASLG faslg for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FASL, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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