Mouse anti-Human Fc Epsilon RI / FcERI Monoclonal Antibody | anti-FCER1A antibody

Mouse Monoclonal [clone AER-37 (CRA1)] (IgG2b,k) to Human Fc Epsilon RI / FcERI

Cat. Num. AAA51848

• Gene Names: FCER1A; FCE1A; FcERI
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry
• Purity: Affinity Purified
• Synonyms: Fc Epsilon RI / FcERI, Antibody; Mouse Monoclonal [clone AER-37 (CRA1)] (IgG2b,k) to Human Fc Epsilon RI / FcERI; Anti-Fc Epsilon RI / FcERI Antibody (clone AER-37 (CRA1)) IHC-plus; Alpha polypeptide; FCE1A; FcERI; Fc Epsilon RI; Fc epsilon RI alpha-chain; Fc-epsilon RI-alpha; IgE Fc receptor subunit alpha; Human Fc Epsilon RI; anti-FCER1A antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2b,k
• Clone Number: AER-37 (CRA1)
• Purity/Purification: Affinity Purified
• Form/Format: PBS, pH 7.2, 150 mM NaCI, 0.09% sodium azide.
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 257

• Applicable Applications for anti-FCER1A antibody: FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry)
• Immunogen Species: Human
• Immunogen: Fc Epsilon RI antibody was raised against human FCER1A.
• Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and AAA Biotech makes no guarantee of the results:; ; Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration: ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Preparation and Storage: Store at 4°C, avoid repeated freeze thaw cycles.

FCM/FACS (Flow Cytometry)

(Staining of human lysed whole blood with Purified Mouse IgG2b isotype control (green histogram) or Purified AER-37 (CRA1) (blue histogram) followed by Biotin Anti-Mouse IgG and Streptavidin-PE. Cells in the lymphocyte gate were used for analysis.)

IHC (Immunohistochemistry)

(Anti-FCER1A antibody IHC of human mast cell in human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 20 ug/ml.)

Related Product Information for anti-FCER1A antibody

The AER-37 (CRA1) antibody has been tested by flow cytometric analysis of peripheral blood leukocytes. This can be used at less than or equal to 1 ug per test. A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Product Categories/Family for anti-FCER1A antibody

Family: Immunoglobulin

NCBI and Uniprot Product Information

• NCBI GI #: 4503675
• NCBI GeneID: 2205
• NCBI Accession #: NP_001992.1
• NCBI GenBank Nucleotide #: NM_002001.3
• UniProt Accession #: P12319
• NCBI Official Full Name: high affinity immunoglobulin epsilon receptor subunit alpha
• NCBI Official Synonym Full Names: Fc fragment of IgE, high affinity I, receptor for; alpha polypeptide
• NCBI Official Symbol: FCER1A
• NCBI Official Synonym Symbols: FCE1A; FcERI
• NCBI Protein Information: high affinity immunoglobulin epsilon receptor subunit alpha; Fc-epsilon RI-alpha; Fc epsilon RI alpha-chain; igE Fc receptor subunit alpha; Fc IgE receptor, alpha polypeptide; high affinity immunoglobulin epsilon receptor alpha-subunit; immunoglobulin E receptor, high-affinity, of mast cells, alpha polypeptide
• UniProt Protein Name: High affinity immunoglobulin epsilon receptor subunit alpha
• UniProt Gene Name: FCER1A
• UniProt Synonym Gene Names: FCE1A; FcERI
• UniProt Entry Name: FCERA_HUMAN