ICC (Immunocytochemistry)
(Immunocytochemistry staining of HeLa cells fixed with 4% Paraformaldehyde and using FEN-1 mouse mAb (dilution 1:400).)
Mouse FEN-1 Monoclonal Antibody | anti-FEN-1 antibody
FEN-1 Mouse mAb
Gene Names
FEN1; MF1; RAD2; FEN-1
Reactivity
Human, Mouse, Rat, Monkey, Hamster
Applications
Immunocytochemistry, Western Blot
Purity
The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.
Synonyms
FEN-1, Antibody; FEN-1 Mouse mAb; DNase IV; FEN-1; FEN1; FEN1_HUMAN; Flap endonuclease 1; Flap structure specific endonuclease 1; Flap structure-specific endonuclease 1; hFEN-1; hFEN1; Maturation factor 1; MF1; Rad2.; anti-FEN-1 antibody
Host
Mouse
Reactivity
Human, Mouse, Rat, Monkey, Hamster
Clonality
Monoclonal
Specificity
This antibody detects endogenous levels of FEN-1 and does not cross-react with related proteins.
Purity/Purification
The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.
Form/Format
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-FEN-1 antibody
ICC (Immunocytochemistry), WB (Western Blot)
Immunogen
Purified recombinant human FEN-1 protein fragments expressed in E coli.
Modification
Unmodified/Total
Preparation and Storage
Store at -20 degree C/1 year
ICC (Immunocytochemistry)
(Immunocytochemistry staining of HeLa cells fixed with 4% Paraformaldehyde and using FEN-1 mouse mAb (dilution 1:400).)
ICC (Immunocytochemistry)
(Immunocytochemistry staining of HeLa cells fixed with 4% Paraformaldehyde and using FEN-1 mouse mAb (dilution 1:400).)
WB (Western Blot)
(Western blot detection of FEN-1 in Hela.Jurkat.3T3.COS7.PC-12.C6.Raw264.7.Raji and CHO-K1 cell lysates using FEN-1 mouse mAb (1:1000 diluted).Predicted band size:45KDa.Observed band size:45KDa.)
WB (Western Blot)
(Western blot detection of FEN-1 in Hela.Jurkat.3T3.COS7.PC-12.C6.Raw264.7.Raji and CHO-K1 cell lysates using FEN-1 mouse mAb (1:1000 diluted).Predicted band size:45KDa.Observed band size:45KDa.)
Related Product Information for anti-FEN-1 antibody
The protein encoded by this gene removes 5' overhanging flaps in DNA repair and processes the 5' ends of Okazaki fragments in lagging strand DNA synthesis. Direct physical interaction between this protein and AP endonuclease 1 during long-patch base excision repair provides coordinated loading of the proteins onto the substrate, thus passing the substrate from one enzyme to another. The protein is a member of the XPG/RAD2 endonuclease family and is one of ten proteins essential for cell-free DNA replication. DNA secondary structure can inhibit flap processing at certain trinucleotide repeats in a length-dependent manner by concealing the 5' end of the flap that is necessary for both binding and cleavage byThe protein encoded by this gene. Therefore, secondary structure can deter the protective function of this protein, leading to site-specific trinucleotide expansions
Product Categories/Family for anti-FEN-1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
42,593 Da
NCBI Official Full Name
flap endonuclease 1
NCBI Official Synonym Full Names
flap structure-specific endonuclease 1
NCBI Official Symbol
FEN1
NCBI Official Synonym Symbols
MF1; RAD2; FEN-1
NCBI Protein Information
flap endonuclease 1; DNase IV; maturation factor 1; maturation factor-1
UniProt Protein Name
Flap endonuclease 1
UniProt Gene Name
FEN1
UniProt Synonym Gene Names
RAD2; FEN-1; MF1; hFEN-1
UniProt Entry Name
FEN1_HUMAN
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Product Notes
The FEN-1 fen1 (Catalog #AAA320388) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The FEN-1 Mouse mAb reacts with Human, Mouse, Rat, Monkey, Hamster and may cross-react with other species as described in the data sheet. AAA Biotech's FEN-1 can be used in a range of immunoassay formats including, but not limited to, ICC (Immunocytochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the FEN-1 fen1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FEN-1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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