Mouse anti-Human Ferritin, Light Chain (FTL) Monoclonal Antibody | anti-FTL antibody

Ferritin, Light Chain (FTL) (Microglia Marker)

Cat. Num. AAA23907

• Gene Names: FTL; LFTD; NBIA3
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry
• Synonyms: Ferritin, Light Chain (FTL), Antibody; Ferritin, Light Chain (FTL) (Microglia Marker); Ferritin L chain; Ferritin L subunit; Ferritin light chain; Ferritin light polypeptide; FTL; LFTD; NBIA3; anti-FTL antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2a Kappa
• Clone Number: FTL/1387
• Specificity: Mammalian ferritins consist of 24 subunits made up of 2 types of polypeptide chains, ferritin heavy chain and ferritin light chain. Ferritin heavy chains catalyze the first step in iron storage, the oxidation of Fe (II), whereas ferritin light chains promote the nucleation of ferrihydrite, enabling storage of Fe (III). Light chain ferritin is involved in cataracts by at least two mechanisms, hereditary hyperferritinemia cataract syndrome, in which light chain ferritin is overexpressed, and oxidative stress, an important factor in the development of ageing-related cataracts.
• Form/Format: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide.
• Sequence Length: 175

• Applicable Applications for anti-FTL antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: IHC: 1-2ug/ml, WB: 2-4ug/ml; ; Western Blot (1-2ug/ml) | Immunohistochemistry (Formalin-fixed)(0.1-0.2ug/ml for 30 min at RT); (Staining of formalin-fixed tissuesrequires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes); Optimal dilution for a specific application should be determined.
• Immunogen: Recombinant human FTL protein fragment (aa 38-165)
• Positive Control: Cerebellum or Testis., HeLa, HePG2, HL-60 or 293T cells. Pancreas, Kidney, Liver
• Research Areas: Cardiovascular, Dendritic Cell Marker, Immuno Oncology, Immunology
• Preparation and Storage: Antibody with azide - Store at 2-8°C

WB (Western Blot)

(Western Blot Analysis of human kidney cell lysate using Ferritin, Light ChainMouse Monoclonal Antibody (FTL/1387).)

Application Data

(Analysis of Protein Array containing more than 19,000 full-length human proteins using Ferritin, Light Chain Mouse Monoclonal Antibody (FTL/1387). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)

SDS-PAGE

(SDS-PAGE Analysis Purified Ferritin, Light Chain Mouse Monoclonal Antibody (FTL/1387). Confirmation of Integrity and Purity of Antibody)

WB (Western Blot)

(Western Blot Analysis of human HeLa Cell lysate using Ferritin, Light Chain Mouse Monoclonal Antibody (FTL/1387).)

IHC (Immunohistochemistry)

(Formalin-fixed, paraffin-embedded Human Prostatestained with Ferritin, Light Chain Mouse Monoclonal Antibody (FTL/1387).)

IHC (Immunohistochemistry)

(Formalin-fixed, paraffin-embedded Human Pancreasstained with Ferritin, Light Chain Mouse Monoclonal Antibody (FTL/1387).)

References

Y.Kaneko,T.Kitamoto,J.Tateishi,K.Yamaguchi. Ferritin immunohistochemistry as a marker for microglia. ActaNeuropathologica. 1989,Volume 79; 129-136.

NCBI and Uniprot Product Information

• NCBI GI #: 20149498
• NCBI GeneID: 2512
• NCBI Accession #: NP_000137.2
• NCBI GenBank Nucleotide #: NM_000146.3
• UniProt Accession #: P02792; Q6IBT7; Q7Z2W1; Q86WI9; Q8WU07; Q96AU9; Q96CU0; Q9BTZ8; B2R4B9
• Molecular Weight: 19-25kDa
• NCBI Official Full Name: ferritin light chain
• NCBI Official Synonym Full Names: ferritin light chain
• NCBI Official Symbol: FTL
• NCBI Official Synonym Symbols: LFTD; NBIA3
• NCBI Protein Information: ferritin light chain
• UniProt Protein Name: Ferritin light chain
• UniProt Gene Name: FTL
• UniProt Synonym Gene Names: Ferritin L subunit