FCM/FACS (Flow Cytometry)
(Flowcytometryusinganti-FerritinantibodyF11(HSF102)(AAA72562). ParaformaldehydefixedMCF7cellspermeabilizedwith0.5%Tritonwerestainedwiththeanti-unknownspecificityantibodyortherabbitIgGversionofF11(HSF102)(AAA72562,blueline)atadilutionof1:100for1hatRT.Afterwashing,theboundantibodywasdetectedusingagoatanti-rabbitIgGAlexaFluor488antibodyatadilutionof1:1000,andthecellswereanalyzedusingaFACSCantoflow-cytometer.)
Ferritin Recombinant Antibody | FTMT recombinant antibody
Anti-Ferritin [F11 (HSF102)], Rabbit IgG, kappa
Gene Names
FTMT; MTF
Reactivity
Human
Applications
ELISA
Purity
Protein A affinity purified
Synonyms
Ferritin, Recombinant Antibody; Anti-Ferritin [F11 (HSF102)], Rabbit IgG, kappa; Ferritin; Anti-Ferritin [F11 (HSF102)]; FTMT; FTL; FTH1; Ferritin mitochondrial; B8; FTMT recombinant antibody
Host
Rabbit
Reactivity
Human
Clonality
Monoclonal
Isotype
Rabbit IgG kappa
Clone Number
F11 (HSF102)
Specificity
This antibody binds human ferritin. This protein catalyzes the oxidation of ferrous iron(II) to ferric iron(III) and stores iron in a soluble, non-toxic, readily available form. Ferritin is important for iron homeostasis.
Purity/Purification
Protein A affinity purified
Form/Format
PBS with 0.02% Proclin 300.
Concentration
1mg/ml (varies by lot)
Applicable Applications for FTMT recombinant antibody
ELISA
Pub PMID
9891990
Preparation and Storage
Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.
FCM/FACS (Flow Cytometry)
(Flowcytometryusinganti-FerritinantibodyF11(HSF102)(AAA72562). ParaformaldehydefixedMCF7cellspermeabilizedwith0.5%Tritonwerestainedwiththeanti-unknownspecificityantibodyortherabbitIgGversionofF11(HSF102)(AAA72562,blueline)atadilutionof1:100for1hatRT.Afterwashing,theboundantibodywasdetectedusingagoatanti-rabbitIgGAlexaFluor488antibodyatadilutionof1:1000,andthecellswereanalyzedusingaFACSCantoflow-cytometer.)
FCM/FACS (Flow Cytometry)
(Flowcytometryusinganti-FerritinantibodyF11(HSF102)(AAA72562). ParaformaldehydefixedMCF7cellspermeabilizedwith0.5%Tritonwerestainedwiththeanti-unknownspecificityantibodyortherabbitIgGversionofF11(HSF102)(AAA72562,blueline)atadilutionof1:100for1hatRT.Afterwashing,theboundantibodywasdetectedusingagoatanti-rabbitIgGAlexaFluor488antibodyatadilutionof1:1000,andthecellswereanalyzedusingaFACSCantoflow-cytometer.)
IF (Immunofluorescence)
(ImmunofluorescencestainingofMCF7cellswithanti-FerritinantibodyF11(AAA72562). ImmunofluorescenceanalysisofparaformaldehydefixedMCF7cells,permeabilizedwith0.15%Triton,onShi-fixcoverslipsstainedwiththechimericrabbitIgGversionofF11(AAA72562)(1:100dilution)for1hfollowedbyAlexaFluor488secondaryantibody(1:1000dilution),showingcytoplasmic/mitochondrialstaining.ThenuclearstainisDAPI(blue).Panelsshow,fromleft-right,top-bottom,AAA72562,DAPI,mergedchannels,andanisotypecontrol.TheisotypecontrolwasanunknownspecificityantibodyfollowedbystainingwithAlexaFluor488secondaryantibody.)
IF (Immunofluorescence)
(ImmunofluorescencestainingofMCF7cellswithanti-FerritinantibodyF11(AAA72562). ImmunofluorescenceanalysisofparaformaldehydefixedMCF7cells,permeabilizedwith0.15%Triton,onShi-fixcoverslipsstainedwiththechimericrabbitIgGversionofF11(AAA72562)(1:100dilution)for1hfollowedbyAlexaFluor488secondaryantibody(1:1000dilution),showingcytoplasmic/mitochondrialstaining.ThenuclearstainisDAPI(blue).Panelsshow,fromleft-right,top-bottom,AAA72562,DAPI,mergedchannels,andanisotypecontrol.TheisotypecontrolwasanunknownspecificityantibodyfollowedbystainingwithAlexaFluor488secondaryantibody.)
Related Product Information for FTMT recombinant antibody
The original antibody was generated by immunizing female Balb/c mice with ferritin extracted from human spleen cells.
References
Bespalov et al. Preparation of single-chained antibodies to human ferritin in Escherichia coli. Mol Biol (Mosk). 1993 Mar-Apr;27(2):451-60. N/A8487773
Note on publication:
Describes the generation of scFv antibodies using E. coli expression system.
Note on publication:
Describes the generation of scFv antibodies using E. coli expression system.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
27,538 Da
NCBI Official Full Name
ferritin, mitochondrial
NCBI Official Synonym Full Names
ferritin mitochondrial
NCBI Official Symbol
FTMT
NCBI Official Synonym Symbols
MTF
NCBI Protein Information
ferritin, mitochondrial
UniProt Protein Name
Ferritin, mitochondrial
UniProt Gene Name
FTMT
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Product Notes
The FTMT ftmt (Catalog #AAA72562) is a Recombinant Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Ferritin [F11 (HSF102)], Rabbit IgG, kappa reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Ferritin can be used in a range of immunoassay formats including, but not limited to, ELISA. Researchers should empirically determine the suitability of the FTMT ftmt for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Ferritin, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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