FCM/FACS (Flow Cytometry)
(Overlay Peak curve showing PC-3 cells stained with AAA279341 (red line) at 1:200. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4 degree C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1:200 dilution for 35min at 4 degree C.Control antibody (green line) was mouse IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.)
FGF1 recombinant antibody
FGF1 Recombinant Monoclonal Antibody
Gene Names
FGF1; AFGF; ECGF; FGFA; ECGFA; ECGFB; FGF-1; HBGF1; HBGF-1; GLIO703; ECGF-beta; FGF-alpha
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Immunofluorescence, ELISA
Purity
Affinity chromatography
Synonyms
FGF1, Recombinant Antibody; FGF1 Recombinant Monoclonal Antibody; FGF1; Acidic fibroblast growth factor antibody; aFGF antibody; Beta endothelial cell growth factor antibody; Beta-endothelial cell growth factor antibody; ECGF antibody; ECGF beta antibody; ECGF-beta antibody; ECGFA antibody; ECGFB antibody; Endothelial Cell Growth Factor alpha antibody; Endothelial Cell Growth Factor beta antibody; FGF 1 antibody; FGF alpha antibody; Fgf1 antibody; FGF1_HUMAN antibody; FGFA antibody; Fibroblast Growth Factor 1 Acidic antibody; Fibroblast growth factor 1 antibody; GLIO703 antibody; HBGF 1 antibody; HBGF-1 antibody; HBGF1 antibody; Heparin binding growth factor 1 antibody; Heparin binding growth factor 1 precursor antibody; Heparin-binding growth factor 1 antibody; FGF1 recombinant antibody
Host
HEK293F Cell
Reactivity
Human
Clonality
Monoclonal
Isotype
mIgG2a
Clone Number
23H4
Purity/Purification
Affinity chromatography
Form/Format
Liquid; 0.03% Proclin 300, 50% glycerol, 0.01M PBS, pH 7.4.
Applicable Applications for FGF1 recombinant antibody
FCM/FACS (Flow Cytometry), IF (Immunofluorescence), ELISA
Antigen Species
Human
Immunogen
Recombinant Human FGF1 protein
Preparation and Storage
Upon receipt, store at -20 degree C or -80 degree C. Avoid repeated freeze.
FCM/FACS (Flow Cytometry)
(Overlay Peak curve showing PC-3 cells stained with AAA279341 (red line) at 1:200. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4 degree C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1:200 dilution for 35min at 4 degree C.Control antibody (green line) was mouse IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.)
FCM/FACS (Flow Cytometry)
(Overlay Peak curve showing PC-3 cells stained with AAA279341 (red line) at 1:200. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4 degree C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1:200 dilution for 35min at 4 degree C.Control antibody (green line) was mouse IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.)
IF (Immunofluorescence)
(Immunofluorescence staining of U251 cell with AAA279341 at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).)
IF (Immunofluorescence)
(Immunofluorescence staining of U251 cell with AAA279341 at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).)
Product Categories/Family for FGF1 recombinant antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
17,460 Da
NCBI Official Full Name
acidic fibroblast growth factor
NCBI Official Synonym Full Names
fibroblast growth factor 1 (acidic)
NCBI Official Symbol
FGF1
NCBI Official Synonym Symbols
AFGF; ECGF; FGFA; ECGFA; ECGFB; FGF-1; HBGF1; HBGF-1; GLIO703; ECGF-beta; FGF-alpha
NCBI Protein Information
fibroblast growth factor 1; heparin-binding growth factor 1; beta-endothelial cell growth factor; endothelial cell growth factor, beta; endothelial cell growth factor, alpha
UniProt Protein Name
Fibroblast growth factor 1
UniProt Gene Name
FGF1
UniProt Synonym Gene Names
FGFA; FGF-1; aFGF; ECGF; HBGF-1
UniProt Entry Name
FGF1_HUMAN
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Product Notes
The FGF1 fgf1 (Catalog #AAA279341) is a Recombinant Antibody produced from HEK293F Cell and is intended for research purposes only. The product is available for immediate purchase. The FGF1 Recombinant Monoclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's FGF1 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IF (Immunofluorescence), ELISA. Researchers should empirically determine the suitability of the FGF1 fgf1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FGF1, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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