FCM/FACS (Flow Cytometry)
(Flow-cytometry using anti-human FGF21 antibody.HepG2 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human FGF21 antibody monoclonal antibody (Catalog # RHJ67201 ,Green Histogram) at a concentration of 5 ?ug/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # PMB96441) and cells analysed on a NovoCyte Flow Cytometer.)
FGF21 recombinant antibody
Anti-Human FGF21 Antibody (SAA2062)
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, ELISA
Purity
>95% by SDS-PAGEProtein A or G purified from cell culture supernatant.
Synonyms
FGF21, Recombinant Antibody; Anti-Human FGF21 Antibody (SAA2062); FGF21; Fibroblast growth factor 21; FGF-21; FGF21 recombinant antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2a, kappa
Clone Number
SAA2062
Purity/Purification
>95% by SDS-PAGE
Protein A or G purified from cell culture supernatant.
Protein A or G purified from cell culture supernatant.
Form/Format
Liquid; 0.01M PBS, pH7.4.
Concentration
1mg/ml (varies by lot)
Applicable Applications for FGF21 recombinant antibody
FCM/FACS (Flow Cytometry), ELISA
Preparation and Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at +4 degree C short term (1-2 weeks). Store at -20 degree C 12 months. Store at -80 degree C long term.
FCM/FACS (Flow Cytometry)
(Flow-cytometry using anti-human FGF21 antibody.HepG2 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human FGF21 antibody monoclonal antibody (Catalog # RHJ67201 ,Green Histogram) at a concentration of 5 ?ug/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # PMB96441) and cells analysed on a NovoCyte Flow Cytometer.)
FCM/FACS (Flow Cytometry)
(Flow-cytometry using anti-human FGF21 antibody.HepG2 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human FGF21 antibody monoclonal antibody (Catalog # RHJ67201 ,Green Histogram) at a concentration of 5 ?ug/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # PMB96441) and cells analysed on a NovoCyte Flow Cytometer.)
Bioactivity
(Detects Human FGF21 in indirect ELISAs.)
Bioactivity
(Detects Human FGF21 in indirect ELISAs.)
Product Categories/Family for FGF21 recombinant antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
22,300 Da
NCBI Official Full Name
fibroblast growth factor 21
NCBI Official Synonym Full Names
fibroblast growth factor 21
NCBI Official Symbol
FGF21
NCBI Protein Information
fibroblast growth factor 21; FGF-21
UniProt Protein Name
Fibroblast growth factor 21
UniProt Gene Name
FGF21
UniProt Synonym Gene Names
FGF-21
UniProt Entry Name
FGF21_HUMAN
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Product Notes
The FGF21 fgf21 (Catalog #AAA120399) is a Recombinant Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-Human FGF21 Antibody (SAA2062) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's FGF21 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), ELISA. Researchers should empirically determine the suitability of the FGF21 fgf21 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FGF21, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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