WB (Western Blot)
(Western blot analysis of different tissue and cell line lysates using mouse mAb to fibrillarin, MO22169. Strong band at ~35kDa corresponds to the fibrillarin protein seen in all cell line lysates. A much weaker band is seen in tissue lysates since fibrillarin is more heavily expressed in rapidly dividing cells.)
Mouse Fibrillarin Monoclonal Antibody | anti-FBL antibody
Fibrillarin
Gene Names
FBL; FIB; FLRN; Nop1; RNU3IP1
Reactivity
Human, Mouse, Rat
Applications
Immunofluorescence, Immunohistochemistry, Western Blot, Immunocytochemistry
Purity
Affinity Purified
Synonyms
Fibrillarin, Antibody; Fibrillarin; anti-FBL antibody
Host
Mouse
Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Isotype
IgG
Purity/Purification
Affinity Purified
Form/Format
Liquid
Sequence Length
321
Applicable Applications for anti-FBL antibody
IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot), ICC (Immunocytochemistry)
Immunogen
Recombinant full length human sequence expressed in and purified from E Coli.
WB (Western Blot)
(Western blot analysis of different tissue and cell line lysates using mouse mAb to fibrillarin, MO22169. Strong band at ~35kDa corresponds to the fibrillarin protein seen in all cell line lysates. A much weaker band is seen in tissue lysates since fibrillarin is more heavily expressed in rapidly dividing cells.)
WB (Western Blot)
(Western blot analysis of different tissue and cell line lysates using mouse mAb to fibrillarin, MO22169. Strong band at ~35kDa corresponds to the fibrillarin protein seen in all cell line lysates. A much weaker band is seen in tissue lysates since fibrillarin is more heavily expressed in rapidly dividing cells.)
IF (Immunofluorescence)
(Immunofluorescent analysis of (A) C6 rat glioma cells and (B) Human embryonic kidney cells stained with mouse mAb to fibrillarin, MO22169, dilution 1:1,000 in red, in both cases costained with chicken pAb to vimentin, CH22108, dilution 1:10,000 in green. The blue is DAPI staining of nuclear DNA.)
IF (Immunofluorescence)
(Immunofluorescent analysis of (A) C6 rat glioma cells and (B) Human embryonic kidney cells stained with mouse mAb to fibrillarin, MO22169, dilution 1:1,000 in red, in both cases costained with chicken pAb to vimentin, CH22108, dilution 1:10,000 in green. The blue is DAPI staining of nuclear DNA.)
Related Product Information for anti-FBL antibody
Nop1p/Fibrillarin was originally identified as a nucleolar protein of bakers yeast, Saccharomyces cerevisiae (accession P15646). The Nop1p protein is 327 amino acids in size (34.5kDa), is essential for yeast viability, and is localized in the nucleoli. Nop1p is the yeast homologue of a protein apparently found in all eukaryotes and archaea generally called fibrillarin. Fibrillarin/Nop1p is extraordinarily conserved, so that the yeast and human proteins are 67% identical, and the human protein can functionally replace the yeast protein. This antibody is becoming widely used as a convenient marker for nucleoli in a wide variety of species (e.g. 4-6). The HGNC name for this protein is FBL. To raise the fibrillarin antibody, mice were injected with full length recombinant human fibrillarin.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
fibrillarin
NCBI Official Synonym Full Names
fibrillarin
NCBI Official Symbol
FBL
NCBI Official Synonym Symbols
FIB; FLRN; Nop1; RNU3IP1
NCBI Protein Information
rRNA 2'-O-methyltransferase fibrillarin
UniProt Protein Name
rRNA 2'-O-methyltransferase fibrillarin
UniProt Gene Name
FBL
UniProt Synonym Gene Names
FIB1; FLRN
UniProt Entry Name
FBRL_HUMAN
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Product Notes
The FBL fbl (Catalog #AAA76609) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Fibrillarin reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Fibrillarin can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot), ICC (Immunocytochemistry). Researchers should empirically determine the suitability of the FBL fbl for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Fibrillarin, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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