Standard Curve (Sample)
(Representative Standard Curve using mouse monoclonal anti-fibronectin (FM0221) for ELISA capture of human recombinant fibronectin protein with His-tag. Capture was detected by using an anti-His-tag antibody followed by appropriate secondary antibody conjugated to HRP.)
Mouse anti-Human Fibronectin Monoclonal Antibody | anti-FN1 antibody
Fibronectin M022 Antibody
Gene Names
FN1; FN; CIG; FNZ; MSF; ED-B; FINC; GFND; LETS; GFND2
Reactivity
Human
Applications
Immunoprecipitation, Immunocytochemistry, ELISA, Western Blot
Purity
Protein G chromatography
Synonyms
Fibronectin, Antibody; Fibronectin M022 Antibody; Fibronectin; Fibronectin M022; anti-FN1 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Clone Number
M022
Specificity
Clone M022 mouse monoclonal antibody detects a 250kDa* protein on SDS-PAGE immunoblots of native or denatured human A549, MeWo, and LNCaP cells, as well as human plasma fibronectin. The antibody also works for immunoprecipitation, immunohistochemistry, and immunocytochemistry, as well as binds fibronectin in live cells and unfixed extracellular matrix.
Purity/Purification
Protein G chromatography
Form/Format
Mouse monoclonal antibody purified with protein G chromatography is supplied in 100ul phosphate-buffered saline, 50% glycerol, 1mg/ml BSA, and 0.05% sodium azide.
Applicable Applications for anti-FN1 antibody
IP (Immunoprecipitation), ICC (Immunocytochemistry), ELISA, WB (Western Blot)
Immunogen
Clone (M022) was generated from a proprietary antigen related to native human fibronectin from the MeWo melanoma cell line.
Preparation and Storage
Store at -20 degree C. Stable for 1 year.
Standard Curve (Sample)
(Representative Standard Curve using mouse monoclonal anti-fibronectin (FM0221) for ELISA capture of human recombinant fibronectin protein with His-tag. Capture was detected by using an anti-His-tag antibody followed by appropriate secondary antibody conjugated to HRP.)
Standard Curve (Sample)
(Representative Standard Curve using mouse monoclonal anti-fibronectin (FM0221) for ELISA capture of human recombinant fibronectin protein with His-tag. Capture was detected by using an anti-His-tag antibody followed by appropriate secondary antibody conjugated to HRP.)
ICC (Immunocytochemistry)
(Immunocytochemical labeling of fibronectin in paraformaldehyde fixed human MeWo cells. The cells were labeled with mouse monoclonal anti-fibronectin (clone M022). The antibody was detected using goat anti-mouse Ig DyLight 594.)
ICC (Immunocytochemistry)
(Immunocytochemical labeling of fibronectin in paraformaldehyde fixed human MeWo cells. The cells were labeled with mouse monoclonal anti-fibronectin (clone M022). The antibody was detected using goat anti-mouse Ig DyLight 594.)
WB (Western Blot)
(Western blot image of human plasma purified fibronectin (lane 1), human breast tissue (lane 2), human A549 cells (lane 3), and LNCaP cells (lane 4). The blot was probed with mouse monoclonal anti-fibronectin FM0221 at 1:500.)
WB (Western Blot)
(Western blot image of human plasma purified fibronectin (lane 1), human breast tissue (lane 2), human A549 cells (lane 3), and LNCaP cells (lane 4). The blot was probed with mouse monoclonal anti-fibronectin FM0221 at 1:500.)
Related Product Information for anti-FN1 antibody
In the extracellular matrix, fibronectin provides essential connections to cells through interaction with integrins and other receptors that regulate cell adhesion, migration, and differentiation. Fibronectin is secreted as a large dimeric glycoprotein with subunits that range in size from 230kDa to 270kDa. Fibronectin is composed of three different types of modules termed type I, II, and III repeats, as well as two fibrin binding and two heparin binding domains, a collagen interaction region and cell attachment domain. The diverse set of binding domains provides fibronectin with the ability to interact simultaneously with other fibronectin molecules, other ECM components (e.g., collagens and proteoglycans), cell surface receptors, and extracellular enzymes. Plasma fibronectin (soluble dimeric form) is secreted by hepatocytes, while cellular fibronectin (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix. Fibronectin fibrilogenesis has important functions during tissue development, and during pathological progression of tissues and wound healing.
References
Zollinger AJ & Smith ML (2017) Matrix Biol. 60-61:27.
Wang Y & Ni H. (2016) Cell Mol Life Sci. 73(17):3265.
Schwarzbauer J & DeSimone D (2011) CSH Persp Biol. 1;3(7).
Wang Y & Ni H. (2016) Cell Mol Life Sci. 73(17):3265.
Schwarzbauer J & DeSimone D (2011) CSH Persp Biol. 1;3(7).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
262,625 Da
NCBI Official Full Name
fibronectin isoform 3 preproprotein
NCBI Official Synonym Full Names
fibronectin 1
NCBI Official Symbol
FN1
NCBI Official Synonym Symbols
FN; CIG; FNZ; MSF; ED-B; FINC; GFND; LETS; GFND2
NCBI Protein Information
fibronectin; cold-insoluble globulin; migration-stimulating factor
UniProt Protein Name
Fibronectin
UniProt Gene Name
FN1
UniProt Synonym Gene Names
FN; FN; CIG
UniProt Entry Name
FINC_HUMAN
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Product Notes
The FN1 fn1 (Catalog #AAA71638) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Fibronectin M022 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Fibronectin can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), ICC (Immunocytochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the FN1 fn1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Fibronectin, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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